Auditory sensitivity of larval zebrafish (<i>Danio rerio</i>) measured using a behavioral prepulse inhibition assay

Bhandiwad, Ashwin A.; Zeddies, David G.; Raible, David W.; Rubel, Edwin W.; Sisneros, Joseph A.

doi:10.1242/jeb.087635

Cited by 91 publications

(84 citation statements)

References 67 publications

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“…Consistent with prior studies using similar stimulation and recording methods (Starr et al, 2004;Tanimoto et al, 2009;Lu and DeSmidt, 2013), and of behavioral audiograms (Bhandiwad et al, 2013), wildtype larvae were responsive to vibratory stimuli over the range of frequencies tested (175-500 Hz, Fig. 2).…”

Section: Morphant Hair Cell Physiology Phenotypessupporting

confidence: 87%

Manipulation of BK channel expression is sufficient to alter auditory hair cell thresholds in larval zebrafish

Rohmann

Tripp

Genova

et al. 2014

Journal of Experimental Biology

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Non-mammalian vertebrates rely on electrical resonance for frequency tuning in auditory hair cells. A key component of the resonance exhibited by these cells is an outward calcium-activated potassium current that flows through large-conductance calciumactivated potassium (BK) channels. Previous work in midshipman fish (Porichthys notatus) has shown that BK expression correlates with seasonal changes in hearing sensitivity and that pharmacologically blocking these channels replicates the natural decreases in sensitivity during the winter non-reproductive season. To test the hypothesis that reducing BK channel function is sufficient to change auditory thresholds in fish, morpholino oligonucleotides (MOs) were used in larval zebrafish (Danio rerio) to alter expression of slo1a and slo1b, duplicate genes coding for the pore-forming α-subunits of BK channels. Following MO injection, microphonic potentials were recorded from the inner ear of larvae. Quantitative real-time PCR was then used to determine the MO effect on slo1a and slo1b expression in these same fish. Knockdown of either slo1a or slo1b resulted in disrupted gene expression and increased auditory thresholds across the same range of frequencies of natural auditory plasticity observed in midshipman. We conclude that interference with the normal expression of individual slo1 genes is sufficient to increase auditory thresholds in zebrafish larvae and that changes in BK channel expression are a direct mechanism for regulation of peripheral hearing sensitivity among fishes.

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Section: Morphant Hair Cell Physiology Phenotypessupporting

confidence: 87%

Manipulation of BK channel expression is sufficient to alter auditory hair cell thresholds in larval zebrafish

Rohmann

Tripp

Genova

et al. 2014

Journal of Experimental Biology

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“…no abnormal rolls, yaws or pitches), as might be expected if hair cells of the vestibular system had been affected by the aminoglycoside treatments. Other studies have likewise shown no effects of aminoglycoside immersion treatments on the inner ear as measured by the hearing abilities of fish (Bhandiwad et al, 2013).…”

Section: Evidence Against Global Toxic or Non-lateral Line Sensory Efmentioning

confidence: 91%

The lateral line is necessary for blind cavefish rheotaxis in non-uniform flow

Kulpa

Bak-Coleman

Coombs

2015

Journal of Experimental Biology

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When encountering a unidirectional flow, many fish exhibit an unconditioned orienting response known as rheotaxis. This multisensory behavior can reportedly involve visual, vestibular, tactile and lateral line cues. However, the precise circumstances under which different senses contribute are still unclear and there is considerable debate, in particular, about the contributions of the lateral line. In this study, we investigate the rheotactic behavior of blind cavefish under conditions of spatially non-uniform flow (a jet stream), which in theory, should promote reliance on lateral line cues. The behavior of individual lateral line enabled and disabled fish was videorecorded under IR light in a square arena that prevented streamwise biases and that contained a narrow jet stream in the center of the tank. Whereas the stream's peak velocity (8 cm s(-1)) declined very little in the streamwise direction, it declined steeply in the cross-stream direction (∼3-4.5 cm s(-1) cm(-1)). Lateral line enabled fish showed higher levels of orientation to the stream and its source (a 1-cm-wide nozzle) when in the central (jet stream) region of the tank compared with surrounding regions, whereas lateral line disabled fish showed random orientations in all regions of the tank. The results of this study indicate that the spatial characteristics of flow play a role in determining the sensory basis of rheotaxis.

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“…The first is the inner ear, and the second is the lateral line, which contains sensory hair cells similar to the human ear. Hearing tests using acoustic stimuli or vibration tests can be performed starting at 5 dpf (Bhandiwad et al 2013). Larvae display a complex behaviour very similar to adult fish as early as 4 dpf.…”

Section: Development Of the Zebrafish Neurosystemmentioning

confidence: 99%

Comparability of behavioural assays using zebrafish larvae to assess neurotoxicity

Legradi

Abdellaoui

Pomeren

et al. 2014

Environ Sci Pollut Res

102

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Testing of compounds for neurotoxicity has become increasingly important in recent years. It has been shown that neurological disorders like autism may be related to chemical exposures, which may play a crucial role in the progression of these diseases. Special attention has been be given to the substances causing developmental neurotoxicity as the developing nervous system is more vulnerable to impacts by chemicals than the adult nervous system. The zebrafish (Danio rerio) is a well-established model species in developmental biology and an emerging model in behavioural and neurological studies. Zebrafish larvae display numerous behavioural patterns highly similar to rodents and humans. Their physical characteristics make them well suited for automated high-throughput screening. In the last years, the number of behavioural studies conducted with zebrafish larvae has increased notably. The goal of this review is to provide an overview of behavioural assays commonly used to test substances for developmental neurotoxicity. Literature from 1995 to 2014 was reviewed and focussed on assays performed with zebrafish larvae younger than 7 days post fertilization (dpf). The behavioural tests were scrutinized, and parameters describing the different experimental setups were defined. In the next step, we investigated if differences in the experimental parameters alter the outcome of the test. In order to test the comparability of behavioural assays, we analysed several studies using ethanol, valproate and pentylenetetrazole as model substances. Based on our findings, we provide recommendations which could help improve future behavioural studies performed with zebrafish larvae.

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Auditory sensitivity of larval zebrafish (Danio rerio) measured using a behavioral prepulse inhibition assay

Cited by 91 publications

References 67 publications

Manipulation of BK channel expression is sufficient to alter auditory hair cell thresholds in larval zebrafish

Manipulation of BK channel expression is sufficient to alter auditory hair cell thresholds in larval zebrafish

The lateral line is necessary for blind cavefish rheotaxis in non-uniform flow

Comparability of behavioural assays using zebrafish larvae to assess neurotoxicity

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