High throughput analysis of grape genetic diversity as a tool for germplasm collection management

Laucou, Valérie; Lacombe, Thierry; Dechesne, Flora; Siret, René; Bruno, Jakubec; Dessup, Mireille; Dessup, Thierry; Ortigosa, Patrick; Parra, Pierre; Roux, Catherine; Santoni, Sylvain; Varès, Didier; Péros, Jean‐Pierre; Boursiquot, Jean‐Michel; This, Patrice

doi:10.1007/s00122-010-1527-y

Cited by 194 publications

(194 citation statements)

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“…2011). Consistent with the assumption that any wild‐collected V. californica with an allele other than 241 bp at VVMD7 is potentially a hybrid, all but one of the thirty‐five CAL hybrids were either heterozygous for the V. californica exclusive 241‐bp allele and a V. vinifera allele or “homozygous” for a V. vinifera allele; the assumption being these are heterozygous with the high‐frequency V. californica null allele; the one exception was a hybrid with the common rootstock ‘Saint George’ (Table S2).…”

Section: Resultsmentioning

confidence: 99%

Hybridization of cultivated Vitis vinifera with wild V. californica and V. girdiana in California

Dangl

Mendum

Yang

et al. 2015

Ecology and Evolution

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Hybridization of introduced domesticates and closely related natives is well documented in annual crops. The widespread introduction of the domesticated grapevine, Vitis vinifera, into California where it overlaps with two native congenerics, with which it is interfertile, provides opportunity to investigate hybridization between woody perennials. Although geographically widespread, the introduction over the past two centuries has been limited to a few elite clonal cultivars, providing a unique opportunity to study the effects of hybridization on the native species. The amount of hybridization with V. vinifera and the genetic diversity of wild‐growing Vitis californica and Vitis girdiana were examined using nineteen microsatellite markers. STRUCTURE analysis was used to define hybrid and introgressed individuals and to analyze genetic structure of the native species. FAMOZ software was used to identify which V. vinifera cultivars served as parents of F 1 hybrids. The three species were clearly distinguished by STRUCTURE analysis. Thirty percent of 119 V. californica vines were hybrids. The domesticated parent was identified for 16 F 1 hybrid vines; the original California cultivar, ‘Mission’, was the parent of eight. Backcrosses were also found, showing introgression into subsequent generations. Similar results were obtained for a small sample of V. girdiana. Removing hybrids greatly reduced the genetic variation of the presumed pure species, among which there was essentially no genetic structure. Limited genetic variability indicates the California natives may be threatened by genetic erosion. The discovery of F 1 hybrids of ‘Mission’, a cultivar not grown in the areas for ~100 years, suggests long generation times for wild vines that, often, grow into expansive liana and propagate by layering, all factors that limit recruitment in populations already disjunct by habitat lose. Hermaphroditic flowers and fruit that is more attractive to birds may favor the production of backcross seed and establishment of introgressed individuals.

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Section: Resultsmentioning

confidence: 99%

Hybridization of cultivated Vitis vinifera with wild V. californica and V. girdiana in California

Dangl

Mendum

Yang

et al. 2015

Ecology and Evolution

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“…De modo a caracterizar as castas açorianas do ponto de vista molecular enviaram-se amostras de folhas para a empresa BIOCANT, que utiliza correntemente 14 marcadores de microssatélites (nSSR): VVS2, VVMD5, VVMD7, VrZAG62, VrZAG79, VVMD25, VVMD27, VVMD32, VVMD28, VVIhb01, VVIn16, VVIp60, VVIh54, VVIq52, 9 pertencentes ao grupo de marcadores standard utilizados pelo projeto VIV C-Vitis International Variety Catalogue, e os restantes 5 pertencentes ao grupo proposto por Laucou et al (2011). Laucou et al (2011) concluíram que um grupo de 9 destes marcadores (VVMD5, VVMD27, VVMD7, VVMD25, VVIh54, VVIp60, VVIn16, VVIb01, VVIq529) seriam suficientes para discriminar entre 2835 perfis de microssatélites analisados, que incluem variedades cultivadas em todo o mundo.…”

Section: Introductionunclassified

“…Laucou et al (2011) concluíram que um grupo de 9 destes marcadores (VVMD5, VVMD27, VVMD7, VVMD25, VVIh54, VVIp60, VVIn16, VVIb01, VVIq529) seriam suficientes para discriminar entre 2835 perfis de microssatélites analisados, que incluem variedades cultivadas em todo o mundo.…”

Section: Introductionunclassified

Castas de videira tradicionais dos Açores: notas sobre a sua origem

Mestre

2016

Ciência Téc. Vitiv.

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RESUMOAs vinhas antigas dos Açores são constituídas essencialmente por 3 castas: 'Verdelho', 'Arinto dos Açores' e 'Terrantez do Pico'. Estas castas apresentam grande singularidade no contexto vitícola nacional, não sendo conhecida a sua presença em vinhas antigas do território continental português. Apesar de ao longo do tempo várias hipóteses terem sido colocadas quanto à origem destas castas, esta continua insuficientemente clarificada. No presente trabalho propõe-se que a hibridação de castas nas vinhas açorianas esteja na origem das castas 'Terrantez do Pico' e 'Arinto dos Açores', com o 'Bastardo' como provável progenitor da primeira e o 'Verdelho' como possível progenitor de ambas. Discute-se ainda a origem da casta 'Verdelho' e a sua ligação com diversas castas do Noroeste da Península Ibérica. SUMMARYThe older vineyards of the Azores have 3 main grape varieties: 'Verdelho', 'Arinto dos Açores' and 'Terrantez do Pico'. These varieties are unique in the national context, without their ancient presence being registered in the Portuguese mainland vineyards. Although over time several hypotheses have been placed on the origin of these varieties, it is in fact unknown. In this work it is proposed, on the basis of literature review, that the hybridisation of varieties in the vineyards of the Azores is at the origin of the varieties 'Terrantez do Pico' and 'Arinto dos Açores', with 'Bastardo' as likely progenitor of the first and the 'Verdelho' as a possible parent of both. It is also discussed the origin of the 'Verdelho' grape variety and its link to several varieties of the northwest of the Iberian Peninsula.Palavras-chave: Açores, Verdelho, Arinto dos Açores, Terrantez do Pico, microssatélites.

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“…They have been extensively used in grape for variety identification in collections, pedigree analysis, or genetic mapping (Karatas et al 2007;Vezzulli et al 2008;Laucou et al 2011;Moreno-Sanz et al 2011) and are very useful for distinguishing grape genotypes and determining genetic relationships among Vitis cultivars and species (Riahi et al 2012). Only a limited number of microsatellite data on oriental cultivars, particularly on Chinese cultivars, has been reported (Goto-Yamamoto et al 2009;Guo et al 2012a, b).…”

mentioning

confidence: 99%

“…In this study, a set of 9 microsatellite markers Laucou et al 2011) was used to investigate genetic polymorphism and relationships among the main local Chinese grape cultivars and some newly bred Chinese cultivars. International cultivars such as Pinot Gris and Cabernet Sauvignon were chosen as a base for comparison.…”

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confidence: 99%

Characterization of grape cultivars from China using microsatellite markers

Guo¹,

Zhang²,

Zhang³

2013

CZECH J GENET PLANT

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Guo D.-L., Zhang Q., Zhang G.-H. (2013): Characterization of grape cultivars from China using microsatellite markers. Czech J. Genet. Plant Breed., 49: 164-170.A total of 32 different grape cultivars including representatives of local Chinese cultivars, some important and widely grown Chinese cultivars and international reference cultivars were genotyped at nine microsatellite loci in order to characterize their genetic diversities. The numbers of alleles detected per locus ranged from 9 to 18 with a total of 105 alleles and an average of 11.7 alleles per locus, while the number of microsatellite genotypes varied between 10 and 23, indicating that there are abundant allele diversities in Chinese grape cultivars. The expected heterozygosity varied between 0.740 and 0.915 and the polymorphism information content ranged from 0.716 to 0.908. According to the results of clustering and Principal Coordinates Analysis, three groups were identified among all these cultivars. The clusters of cultivars showed a clear separation of table grape, wine grape of Vitis vinifera and hybrids between European and American species. This study generated a microsatellite profile database for the cultivars from Chinese local and newly bred grapes.

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High throughput analysis of grape genetic diversity as a tool for germplasm collection management

Cited by 194 publications

References 50 publications

Hybridization of cultivated Vitis vinifera with wild V. californica and V. girdiana in California

Hybridization of cultivated Vitis vinifera with wild V. californica and V. girdiana in California

Castas de videira tradicionais dos Açores: notas sobre a sua origem

Characterization of grape cultivars from China using microsatellite markers

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