High temperature liquid chromatography hyphenated with ESI-MS and ICP-MS detection for the structural characterization and quantification of halogen containing drug metabolites

Vlieger, Jon S. B. de; Giezen, Mark J.N.; Falck, David; Tump, Cornelis; Heuveln, Fred van; Giera, Martin; Kool, Jeroen; Lingeman, H.; Wieling, Jaap; Honing, Maarten; Irth, H.; Niessen, W.M.A.

doi:10.1016/j.aca.2011.04.053

Cited by 25 publications

(16 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In order to overcome these effects, several approaches have been developed, ranging from the use of cooled spray chambers to counter gradient systems [26][27][28][29][30][31]; another interesting approach that prevents plasma instabilities and was reported recently by De Vlieger et al is based on high-temperature liquid chromatography (HTLC) with aqueous solvents [32] and temperature gradients during separation; under high-temperature conditions, the aqueous phase changes its properties and "quasi" RP-HPLC conditions are generated almost without the need for organic solvent addition. Next to approaches for adapting organic solvent load isobaric interferences should also be considered, especially when using low-resolution ICP-MS instruments.…”

Section: Hplc/icp-msmentioning

confidence: 99%

Hyphenated techniques as tools for speciation analysis of metal-based pharmaceuticals: developments and applications

Meermann

Sperling

2012

Anal Bioanal Chem

View full text Add to dashboard Cite

Method development and applications of hyphenated techniques as tools for speciation analysis of metal-based pharmaceuticals are summarized within this review. Advantages and limitations of the separation modes-high-performance liquid chromatography (HPLC), capillary electrophoresis (CE), and gas chromatography (GC)-as well as the detection modes-inductively coupled plasma-mass spectrometry (ICP-MS) and electrospray ionization-mass spectrometry (ESI-MS)-are discussed. ICP-MS detection is found to be advantageous for the quantification of drugs containing metals and other heteroatoms. The species-independent sensitivity and multielement capabilities of ICP-MS allow it to be used for quantification even when species-specific standards are not available, as well as to determine the stoichiometry in metallodrug-biomolecule interactions. Molecular information that is totally destroyed when ICP is applied as ionization source and is therefore not obtainable via ICP-MS detection can be accessed by the complementary technique of ESI-MS. Speciation analysis combining both elemental and molecular information is therefore a powerful tool for the analysis of metal-based pharmaceuticals and their metabolites in body fluids and other relevant matrices.

show abstract

Section: Hplc/icp-msmentioning

confidence: 99%

Hyphenated techniques as tools for speciation analysis of metal-based pharmaceuticals: developments and applications

Meermann

Sperling

2012

Anal Bioanal Chem

View full text Add to dashboard Cite

show abstract

“…Therefore, when it is necessary to identify or quantify a halogen or halogenated compound, a detection technique that provides sufficient selectivity, sensitivity, and suitable LODs must be coupled with a separation technique [18]. The chromatographic approach used for determination of halogens is mainly related to speciation analysis of organic halogens compounds, such as pesticides and metabolites [174], which is, however, beyond the scope of this review.…”

Section: Coupled Techniquesmentioning

confidence: 99%

Analytical methods for the determination of halogens in bioanalytical sciences: a review

et al. 2013

View full text Add to dashboard Cite

Fluorine, chlorine, bromine, and iodine have been studied in biological samples and other related matrices owing to the need to understand the biochemical effects in living organisms. In this review, the works published in last 20 years are covered, and the main topics related to sample preparation methods and analytical techniques commonly used for fluorine, chlorine, bromine, and iodine determination in biological samples, food, drugs, and plants used as food or with medical applications are discussed. The commonest sample preparation methods, as extraction and decomposition using combustion and pyrohydrolysis, are reviewed, as well as spectrometric and electroanalytical techniques, spectrophotometry, total reflection X-ray fluorescence, neutron activation analysis, and separation systems using chromatography and electrophoresis. On this aspect, the main analytical challenges and drawbacks are highlighted. A discussion related to the availability of certified reference materials for evaluation of accuracy is also included, as well as a discussion of the official methods used as references for the determination of halogens in the samples covered in this review.

show abstract

“…LC-ICP/MS technology could represent an advantage in drug metabolism studies due to its ability to perform mass balance studies without radiolabeling compounds with 14 C and 3 H and may be considered as an alternative to expensive techniques such as accelerated mass spectrometry. Thus, the combination of LC and ICP/MS has been recently introduced as a promising technique for the analysis of drug compounds and their metabolites containing an element detectable by ICP/MS such as sulfur [6][7][8][9], phosphorus [10,11], chlorine [6], iodine [7,12], and bromine [3,[13][14][15][16][17][18]. Among them, sulfur is one of the most interesting elements in terms of potential application of LC-ICP/MS to drug metabolism studies.…”

Section: Introductionmentioning

confidence: 99%

Determination of S-containing drug metabolites from in vitro and in vivo metabolism studies by using LC-ICP/MS

et al. 2012

View full text Add to dashboard Cite

Recently, liquid chromatography coupled to inductively coupled plasma mass spectrometry (LC-ICP/MS) has been introduced to deal with some applications in the field of pharmaceutical, biomedical, and clinical analysis. In the case of drug research, the number of drugs and their metabolites containing detectable elements is quite limited. In this paper, LC-ICP/MS has been demonstrated to be suitable for the determination of S-containing drugs and their metabolites. In order to minimize the interference of polyatomic oxygen (m/z 32), the indirect detection of S, by means of the SO(+) ion (m/z 48), was optimized. For quantification purposes, it has been encountered that the percentage of organic solvent in the mobile phase strongly affects the sensitivity. Here, corrective strategies based on calibration curves established at different solvent concentrations (solvent-zone quantification) and post-column gradient compensation have been proposed to circumvent sensitivity variations. Results obtained have shown that suitable calibration models have been built for any compound regardless of the solvent percentage at which it is eluted from the chromatographic column. To prove the applicability of this methodology, the metabolism of ethacrynic acid and tiotropium bromide has been studied in vitro and in vivo. In the first case, ethacrynic acid does not contain S in its structure, however, the major route of metabolism for this compound consists of the formation of glutathione adduct and its further degradation. In the second case, tiotropium bromide contains two S atoms in its structure.

show abstract

High temperature liquid chromatography hyphenated with ESI-MS and ICP-MS detection for the structural characterization and quantification of halogen containing drug metabolites

Cited by 25 publications

References 26 publications

Hyphenated techniques as tools for speciation analysis of metal-based pharmaceuticals: developments and applications

Hyphenated techniques as tools for speciation analysis of metal-based pharmaceuticals: developments and applications

Analytical methods for the determination of halogens in bioanalytical sciences: a review

Determination of S-containing drug metabolites from in vitro and in vivo metabolism studies by using LC-ICP/MS

Contact Info

Product

Resources

About