High sero-prevalence of caseous lymphadenitis identified in slaughterhouse samples as a consequence of deficiencies in sheep farm management in the state of Minas Gerais, Brazil

Guimarães, Alexandre Silva; Carmo, Filipe Borges do; Heinemann, Marcos Bryan; Portela, Ricardo Wagner; Meyer, Roberto; Lage, Andrey Pereira; Seyffert, Núbia; Miyoshi, Anderson; Azevedo, Vasco; Gouveia, Aurora Maria Guimarães

doi:10.1186/1746-6148-7-68

Cited by 36 publications

(35 citation statements)

References 10 publications

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“…This is may predispose for wound-infection of tick-bite injury as well as mangeinduced injuries resulting from repeated rubbing against hard objects. Similarly, many authors had reported the same findings (Radwan et al, 1989;Guimarães et al, 2011;Zidan et al, 2013).…”

Section: Discussionsupporting

confidence: 58%

Caseous Lymphadenitis in Sudanese and Somalian Camels Imported for Meat Consumption in Egypt

Borham¹,

Oreiby²,

El-Gedawy³

et al. 2017

AJVS

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Key words:CLA camel clinical postmortem microbiological histopathology This study was conducted on Sudanese and Somalian imported camels at AlWarraq abattoir, Giza (Egypt), during the period from September 2014 to November 2015. A total of 850 adult one-humped camels (Camelus dromedarius) were subjected to clinical and postmortem examinations. Caseous lymphadenitis lesions were detected in 88 (10.35%) animals. Superficial form (9.76%) was significantly more prevalent than the visceral form (0.58%). Incised lesions showed cheesy greenish-whitish pus, four lesions were congested without abscess formation. Coryneform bacteria were isolated as a pure culture in 11 (30.55%) cases. Targeting Corynebacterium pseudotuberculosis serotype 1, being a common cause of caseous lymphadenitis, two isolates were identified by pld gene-specific polymerase chain reaction. Other coryneforms were tested by the API Coryne system; three Cellulomonas spp./Microbacterium spp. and one Brevibacterium spp. were identified. The APIunidentified bacteria were subjected to the Vitek 2 system which identified two isolates as Corynebacterium jeikeium and Corynebacterium urealyticum. The Vitek 2 systemunidentified isolates were classified as unidentified coryneforms. Histopathological findings revealed suppuration, necrosis, hyperplastic lymphoid follicles and sinus histocytosis of the affected lymph nodes. In contrast to small ruminants, few studies are available on caseous lymphadenitis in camel and rare of them involve the visceral form. In conclusion, caseous lymphadenitis is prevalent in Sudanese and Somali imported camels in Egypt. In camels, the disease has variable natures and visceral lesions are uncommon. Coryneform bacteria other than Corynebacterium pseudotu1berculosis are associated with the disease in camels which necessitate multiple identification steps.

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Section: Discussionsupporting

confidence: 58%

Caseous Lymphadenitis in Sudanese and Somalian Camels Imported for Meat Consumption in Egypt

Borham¹,

Oreiby²,

El-Gedawy³

et al. 2017

AJVS

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“…Different studies have also reported similar pathological lesions in infected animals (Pratt et al, 2005;Quino de Sa et al, 2013;Chikhaoui and Khoudja, 2013). C. pseudotuberculosis also causes liver abscess, skin and lymph nodes lesions in human, sheep, alpaca and buffaloes (Paton et al, 2003;Guimarães et al, 2011;Moussa et al, 2016). Previously abscess formation in 80-87% of internal and external lymph of sheep, alpacas and prescapular and scapular lymph nodes of infected buffaloes due to C. pseudotuberculosishas been reported (Anderson et al, 2004;Moussa et al, 2016).…”

Section: Resultsmentioning

confidence: 75%

Clinico-Pathological and Bacteriological Studies on Caseous Lymphadenitis in Small Ruminants

Hussain¹

2017

PAKJAS

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Corynebacterium pseudotuberculosis is an intracellular bacterium, proliferates inside the macrophages, causes caseous lymphadenitis in small ruminants and is also responsible for human lymphadenitis. In current study, clinical signs and necropsy lesions suggestive of caseous lymphadenitis were observed in Chinkara deer (n=36), spotted deer (n=04) and Mouflen sheep (n=04) in a period of three years. Different pus samples were obtained from various superficial and internal lymph nodes, lungs, liver and spleen at the time of postmortem examination. All the collected samples were processed for microbiological investigations. C. pseudotuberculosis bacterium was cultured and identified in a total of 33 (75%) animals having clinical and necropsy lesions. The presence of C. pseudotuberculosis infection was significantly higher in older animals as compared to young animals. Morphologically, colonies were brown-yellowish in color, non-hemolytic on 5% sheep blood agar and biochemical characteristics were similar to C. pseudotuberculosis except urea test. Necropsy showed presence of different tubercles containing caseous material in visceral organs such as liver, spleen, lungs and intestinal mesenteric lymph nodes. Microscopically, tissue sections obtained from lungs, spleen, liver and intestinal mesenteric lymph nodes infected from C. pseudotuberculosis were plugged with abscess. The findings of our study indicate that wild animals are a reservoir for zoonotic Corynebacterium.

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“…Despite the economic [1, 2] and zoonotic [3] relevance of CL, a satisfactory vaccine model has not been developed [4, 5]. …”

Section: Introductionmentioning

confidence: 99%

Humoral and cellular immune responses in mice against secreted and somatic antigens from a Corynebacterium pseudotuberculosis attenuated strain: Immune response against a C. pseudotuberculosis strain

et al. 2016

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BackgroundCorynebacterium pseudotuberculosis is the etiologic agent of caseous lymphadenitis (CL), a chronic disease that affects goats and sheep. CL is characterized by the formation of granulomas in lymph nodes and other organs, such as the lungs and liver. Current knowledge of CL pathogenesis indicates that the induction of humoral and cellular immune responses are fundamental to disease control. The aim of this study was to evaluate the humoral and cellular immune responses in BALB/c mice inoculated with a C. pseudotuberculosis strain isolated in the state of Bahia, Brazil.ResultsThe lymphocyte proliferation and in vitro production of IFN-γ, IL-4, IL-10, IL-12 and nitric oxide by spleen cells stimulated with secreted and somatic antigens from the studied strain were evaluated. IgG subclasses were also analyzed. Results showed a significant increase of Th1-profile cytokines after 60 days post-inoculation, as well as an important humoral response, represented by high levels of IgG2a and IgG1 against C. pseudotuberculosis.ConclusionThe T1 strain of C. pseudotuberculosis was shown to induce humoral and cellular immune responses in BALB/c mice, but, even at a dosage of 1x107 CFU, no signs of the disease were observed.

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High sero-prevalence of caseous lymphadenitis identified in slaughterhouse samples as a consequence of deficiencies in sheep farm management in the state of Minas Gerais, Brazil

Cited by 36 publications

References 10 publications

Caseous Lymphadenitis in Sudanese and Somalian Camels Imported for Meat Consumption in Egypt

Caseous Lymphadenitis in Sudanese and Somalian Camels Imported for Meat Consumption in Egypt

Clinico-Pathological and Bacteriological Studies on Caseous Lymphadenitis in Small Ruminants

Humoral and cellular immune responses in mice against secreted and somatic antigens from a Corynebacterium pseudotuberculosis attenuated strain: Immune response against a C. pseudotuberculosis strain

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