High‐sensitivity spectrofluorimetric determination of tiopronin based on inhibition of hemoglobin

Abstract

A highly sensitive and simple spectrofluorimetric method for the determination of tiopronin based on its inhibitory effect on the hemoglobin-catalyzed reaction of H(2)O(2) and L-tyrosine was developed. The concentration of tiopronin is linear with decreased fluorescence (ΔF) of the system under the optimal experimental conditions. The calibration graph is linear in the range 1.23 × 10(-8) to 3.06 × 10(-5) mol L(-1) with a detection limit of 6.13 × 10(-9) mol L(-1). The relative standard deviation was 4.38% for 11 determinations of 6.13 × 10(-6) mol L(-1). This method can be used for the determination of tiopronin in pharmaceuticals with satisfactory results.

Determination of tiopronin based on the enhancement of Ru(bpy) 3 2+ co-reactant electrochemiluminescence

“…Compared with the previous methods for the determination of tiopronin [3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18], the method in this paper is simple, rapid, with a wide linear range and low detection limit. The proposed method was applied to the determination of tiopronin in pharmaceutical preparations，and the results were satisfactory with recoveries of 91.7±1.7%, 98.3±1.0% and 100.8±0.5%, respectively, for three different concentration levels (0.61 µmol L -1 , 6.1 µmol L -1 and 12.2 µmol L -1 ).…”

“…Spectrophotometric and chemiluminescent methods are simple but relatively insensitive [3,16,17]. The fluorometric methods are more sensitive, but complex chemical reactions are required in most cases [4][5][6][7][8]. Modern instrumental analytical techniques such as CE [9], HPLC [10][11][12], LC [13][14][15] methods are powerful separation methods that could be coupled with different detectors, but they involve relatively expensive instruments, complicate operations and are time-consuming.…”

“…Therefore, a simple, rapid, sensitive and reliable method for tiopronin determination is of great importance in order to address the potential prevention of adverse effects. Up to now, several methods have been used for tiopronin measurements, such as spectrophotometry [3], fluorescence [4][5][6][7][8], CE-UV [9], HPLC-UV [10][11][12], LC-ESI-MS [13][14][15], chemiluminescence [16,17] and Amperometric flow injection analysis [18], etc. Spectrophotometric and chemiluminescent methods are simple but relatively insensitive [3,16,17].…”

Determination of selenium via the fluorescence quenching effect of selenium on hemoglobin‐catalyzed peroxidative reaction

“…In a recent paper, Hb was used as a mimetic enzyme for HRP because they have similar spatial structure and Hb is cheaper and more stable than HRP . The reaction of tyrosine with H 2 O 2 to form the product S, a strong fluorescence substance, using Hb as a catalyst has been reported .…”

A simple and sensitive turn‐on fluorescence probe for detection of cetyltrimethylammonium bromide in aqueous samples

“…To some extent, there exist some limitations related to these methods, including relatively time‐consuming and tedious procedures that require expensive equipment, unsatisfactory selectivity and sensitivity. Therefore, fluorescence methodologies have achieved extensive interest because of their advantages in terms of simplicity, sensitivity, selectivity, measurements, and real time and on‐line detection . However, most fluorescent chem‐/biosensors are based on fluorescence quenching as the detection mechanism .…”