High sensitivity immunoassays for small molecule compounds detection – Novel noncompetitive immunoassay designs

Li, Yanshen; Zhang, Guizhen; Mao, Xin; Yang, Shupeng; Ruyck, Karl De; Wu, Yongning

doi:10.1016/j.trac.2018.04.008

Cited by 38 publications

(33 citation statements)

References 93 publications

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“…The quantity of a product, equivalent to the concentration of the antibody-antigen complex, is proportional or inversely proportional (depending on the type of test) to the quantity of the given substance in the sample. There are several types of ELISA test-based on direct reaction (noncompetitive, competitive with catching antibodies, competitive with catching antigen), indirect reaction (normal, with the avidin-biotin system, with the enzyme-antienzyme complex, e.g., PAP), "sandwich"-type test and competitive or non-competitive reaction [39][40][41]. Basic types of ELISA are presented in Fig.…”

Section: Enzyme-linked Immunosorbent Assay (Elisa)mentioning

confidence: 99%

Review: immunoassays in DNA damage and instability detection

Boguszewska

Szewczuk

Urbaniak

et al. 2019

Cell. Mol. Life Sci.

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The review includes information on the current state of knowledge of immunometric methods with emphasis on the possibility of deoxyribonucleic acid (DNA) damage detection. Beginning with basic immunoassay enzyme-linked immunosorbent assay (ELISA), this review describes methods such as tyramide signal amplification (TSA), enhanced polymer one-step staining (EPOS), and time resolved amplified cryptate emission (TRACE) as improvements of ELISA's developed over time to obtain more accurate results. In the second part of the review, surface plasmon resonance (SPR) and quantum dots (QDs) are presented as the newest outlooks in the context of immunoanalysis of biological material and molecular studies. The aim of this review is to briefly present immunoassays with emphasis on DNA damage detection; therefore, the types of methods are listed and described, types of signal indicators, basic definitions such as antigen and antibody are given. Every method is considered with an exemplary application focusing on DNA studies, DNA damage and instability detection.

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Section: Enzyme-linked Immunosorbent Assay (Elisa)mentioning

confidence: 99%

Review: immunoassays in DNA damage and instability detection

Boguszewska

Szewczuk

Urbaniak

et al. 2019

Cell. Mol. Life Sci.

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“…Thus, researchers have sought to develop noncompetitive immunoassays for the determination of small molecules. Despite these potential advantages, the noncompetitive immunoassay system is unsuitable for measuring small molecules with a single antigenic determinant (epitope) called haptens, such as toxins, vitamins, drugs, eicosanoids, and industrial chemicals (Akter, Vehniäinen, Kankaanpää, & Lamminmäki, 2017;Li et al, 2018). Several attempts have been made to overcome this difficulty, and several novel immunoassays that can noncompetitively detect haptens have been developed.…”

Section: Introductionmentioning

confidence: 99%

Development of a noncompetitive idiometric nanobodies phage immumoassay for the determination of fumonisin B₁

Shu

Jiang

et al. 2019

Food and Agricultural Immunology

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Here we demonstrate a noncompetitive idiometric nanobodies phage immunoassay for the determination of FB 1 , utilizing two types of anti-idiotypic nanobody, that is specific for different epitopes within the hypervariable region of the primary mAb. Three alphatype anti-idiotypic nanobodies were obtained from a naive nanobodies phage-display library. A noncompetitive idiometric immunoassay was established with a combination of betatype antiidiotypic nanobody and phage-displayed alphatype anti-idiotypic nanobody. The half-maximal saturation of the signal value (SC 50) was 0.68 ng/mL, with the limit of detection (LOD) was 0.19 ng/mL. When the noncompetitive assay was compared to the competitive ELISA (LOD = 3.41 ng/mL), an over 17-fold improvement in sensitivity was observed. A correlation (R 2) was 0.988 between the data of the noncompetitive assay and LC-MS/MS for the determination of FB 1 in cereal samples. The noncompetitive idiometric immunoassay would have a broad utility for monitoring small molecules in food and environment.

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“…These methods offer highly sensitive and accurate analytical performance; however, they also have disadvantages such as complex operating preprocesses, high cost, and requirement of professional operators. Therefore, simple, cost-effective, and efficient methods for monitoring of PSP toxins have been developed using diverse sensing platforms, e.g., lab-on-a-chip, surface plasmon resonance (SPR), electrochemistry, and fluorescence assay [16,17,18,19,20,21]. However, these PSP-sensing tools still face limitations, e.g., a relatively narrow linear detection range and decreasing signal-based analysis.…”

Section: Introductionmentioning

confidence: 99%

“…In the case of the observation of the signal change that decreases from the control condition, the unstable initial signal degree at the control condition may be a critical problem. Thus, this method is less quantitative unlike an increasing signal-based analysis, where the starting point is a zero signal [21].…”

Section: Introductionmentioning

confidence: 99%

Label-Free Direct Detection of Saxitoxin Based on a Localized Surface Plasmon Resonance Aptasensor

Park

Lee

et al. 2019

Toxins

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Seafood is an emerging health food, and interest in improving the quality of seafood is increasing. Saxitoxin (STX) is a neurotoxin produced by marine dinoflagellates that is accumulated in seafood. It can block the neuronal transmission between nerves and muscle cell membranes, resulting in the disturbance of neuromuscular transmission and subsequent voluntary muscle paralysis. Here, we developed a new aptamer for the detection of STX using graphene oxide–systematic evolution of ligands by exponential enrichment (GO-SELEX). Furthermore, we confirmed sensitivity and selectivity of the developed aptamer specific to STX using a localized surface plasmon resonance (LSPR) sensor. The sensing chip was fabricated by fixing the new STX aptamer immobilized on the gold nanorod (GNR) substrate. The STX LSPR aptasensor showed a broad, linear detection range from 5 to 10,000 μg/L, with a limit of detection (LOD) of 2.46 μg/L (3σ). Moreover, it was suitable for the detection of STX (10, 100, and 2000 μg/L) in spiked mussel samples and showed a good recovery rate (96.13–116.05%). The results demonstrated that the new STX aptamer-modified GNR chip was sufficiently sensitive and selective to detect STX and can be applied to real samples as well. This LSPR aptasensor is a simple, label-free, cost-effective sensing system with a wide detectable range.

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High sensitivity immunoassays for small molecule compounds detection – Novel noncompetitive immunoassay designs

Cited by 38 publications

References 93 publications

Review: immunoassays in DNA damage and instability detection

Review: immunoassays in DNA damage and instability detection

Development of a noncompetitive idiometric nanobodies phage immumoassay for the determination of fumonisin B₁

Label-Free Direct Detection of Saxitoxin Based on a Localized Surface Plasmon Resonance Aptasensor

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High sensitivity immunoassays for small molecule compounds detection – Novel noncompetitive immunoassay designs

Cited by 38 publications

References 93 publications

Review: immunoassays in DNA damage and instability detection

Review: immunoassays in DNA damage and instability detection

Development of a noncompetitive idiometric nanobodies phage immumoassay for the determination of fumonisin B1

Label-Free Direct Detection of Saxitoxin Based on a Localized Surface Plasmon Resonance Aptasensor

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Product

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Development of a noncompetitive idiometric nanobodies phage immumoassay for the determination of fumonisin B₁