High sensitivity detection of salivary 8‐hydroxy deoxyguanosine levels in patients with chronic periodontitis

Abstract

LC-MS/MS is a reliable and sensitive method for evaluating salivary 8-OHdG levels to monitor the treatment response of periodontitis.

Prognostic Value of 8‐Hydroxy‐2′‐Deoxyguanosine and Human Neutrophil Elastase/α1‐Proteinase Inhibitor Complex as Salivary Biomarkers of Oxidative Stress in Chronic Periodontitis

“…Nevertheless, there is no general consensus concerning the correlation among concentrations of 8‐OHdG or HNE/α1‐PI and clinical measurements of disease in terms of PD, CAL, and extent and severity of periodontal breakdown. Although some researchers failed to report significant correlations among concentrations of 8‐OHdG and clinical measurements, 12 , 30 the present results agree with previous reports that demonstrated significant positive correlations for 8‐OHdG 14,16,17,27 and HNE/α1‐PI complex 28 regarding periodontal clinical status. Because concentrations of oxidative stress–related biomarkers may be modified by a variety of mechanisms, a partial explanation for the disagreement could be attributed to the differences in sampling methods and protocols, processing methodology, sensitivity/specificity of the immunoassays, and sample sizes, as well as intra‐individual differences among study populations.…”

“…The majority of tissue breakdown observed in CP is considered to be the result of an altered inflammatory/immune response to microbial plaque that involves not only the massive release of PMNs, free radicals, ROS, and proteinases, but also the reduction of antioxidant enzyme levels and defects in the DNA restoration mechanism that could increase the oxidative damage in periodontal tissues 6,18,19,34,40,41 . Consistent with the data presented herein, all the available studies except for the study of Dede et al 34 collectively indicate that salivary levels of 8‐OHdG are increased in those with periodontitis compared with periodontally healthy individuals 11,12,14‐17,30 . Moreover, although nothing is known about the salivary concentration of HNE/α1‐PI in relation to CP to establish a base of comparison, the levels of HNE/α1‐PI found in saliva of both healthy and periodontally diseased individuals appear to correspond with those found in GCF by others 36 using analog detection techniques.…”

“…ROS generation secondary to PMN infiltration, a well‐known host defense mechanism against bacterial invasion, 6,13,30,31 has been thoroughly implicated in the breakdown of periodontal tissues 13 . Characteristic markers including immunoglobulins, 32 , 33 8‐OHdG, 8,12‐17,27,30,34,35 enzymes, 15,18,28,29,35‐37 and bacterial byproducts 11 , 38 , 39 have been described in diseased periodontal tissues, saliva, gingival crevicular fluid (GCF), and serum samples. Although the available information has demonstrated positive associations between the levels of 8‐OHdG and HNE/α1‐PI regarding CP, it is difficult to establish firm conclusions, because the number of studies concerning differences about these two biomarkers in saliva is too limited.…”

“…One of the byproducts of oxidative damage of DNA in the nucleus is 8‐hydroxy‐2′‐deoxyguanosine (8‐OHdG), which arises from specific enzymatic cleavage after 8‐hydroxylation of guanine 10 . Several studies have indicated that increased 8‐OHdG levels in body fluids, including saliva, constitute a reliable biomarker of oxidative stress 11–17 …”

Levels of oxidative stress biomarkers and bone resorption regulators in apical periodontitis lesions infected by Epstein–Barr virus

“… , Kurgan et al . ), which might be expected, as these two clinical entities have several common mechanisms of pathogenesis.…”