Automated Cell-Based Quantitation of 8-OHdG Damage

Abstract

Detection of 8-OHdG-base damage has been a big challenge for decades, though different analytical methods are developed. The recent approaches that are used for quantitating either the total amount of base damage or the amount of base damage per cell from different sources of samples are not automated. We have developed a method for automated damage detection from a single cell and applied it to 8-OHdG quantitation.

Mesenchymal stem cells-derived extracellular vesicles protect against oxidative stress-induced xenogeneic biological root injury via adaptive regulation of the PI3K/Akt/NRF2 pathway

“…This cycle results in considerable ROS generation, which directly causes oxidative damage to DNA, proteins, and lipids and pathological changes to the tissue [ 47 , 48 ]. To further detect the level of oxidative stress in cells, we can detect the expression of 8-OHdG, 3-NT, and MDA, which are biomarkers for DNA oxidative damage [ 49 ], protein oxidation [ 50 ], and lipid oxidative damage, respectively [ 51 ]. Here, the significantly increased expression of these biomarkers confirmed that xECM and H 2 O 2 induced oxidative damage to seed cells.…”