Clinical intervention in periodontitis may be beneficial for periodontitis patients' systemic oxidative stress control, and using lipidic agents for the use of anti-inflammatory/pro-resolving processes for blocking the actions of arachidonic acid cascade can enable some late therapeutic strategies in order to lead oxidative stress-induced inflammation.

Non-surgical periodontal therapy of patients with RA with periodontitis may provide beneficial effects on local inflammatory control via decreases in gingival crevicular fluid MMP-8, PGE2 and IL-6 levels.

In this study, serum MDA and 8-OHdG were found to be highest in the HLp group. The increased levels of MDA and 8-OHdG in HLp patients may be a result of a harmful oxidative status in association with hyperlipidemia and periodontitis.

LC-MS/MS is a reliable and sensitive method for evaluating salivary 8-OHdG levels to monitor the treatment response of periodontitis.

Background Non‐invasive methods for periodontitis diagnosis would be a clinically important tool. This cross‐sectional study aimed to investigate the association between oxidative stress, glycation, and inflammation markers and periodontal clinical parameters in periodontitis and periodontally healthy patients with type 2 diabetes and corresponding systemically healthy controls. Methods Sixty‐seven periodontally healthy (DM‐H, n = 32) and periodontitis (DM‐P, n = 35) patients with type 2 diabetes, and 54 systemically healthy periodontitis (H‐P, n = 26) and periodontally healthy (H‐H, n = 28) controls were included. Clinical periodontal parameters, body mass index, fasting glucose, hemoglobin A1c (HbA1c), along with saliva and serum 8‐hydroxy‐2′‐deoxyguanosine (8‐OHdG), malondialdehyde (MDA), 4‐hydroxy‐2‐nonenal (4‐HNE), advanced glycation end products (AGE), AGE receptor (RAGE) and high sensitivity C‐reactive protein (hsCRP) levels were recorded and analyzed. Results Salivary 8‐OHdG levels were significantly higher in periodontitis compared to periodontally healthy patients, regardless of systemic status (P < 0.001). Salivary MDA levels were significantly higher in all disease groups compared to H‐H group (P ≤ 0.004). Serum AGE levels were significantly higher in diabetic groups than systemically healthy groups (P < 0.001) and in H‐P compared to H‐H (P < 0.001). Bleeding on probing (BOP) and clinical attachment level (CAL) strongly correlated with salivary 8‐OHdG and serum hsCRP (P < 0.001). In systemically healthy patients, salivary 8‐OHdG was the most accurate marker to differentiate periodontitis from controls (AUC = 0.84). In diabetics salivary 4‐HNE and RAGE were the most accurate (AUC = 0.85 for both). Conclusion Salivary 8‐OHdG alone or in combination with 4‐HNE, AGE and RAGE for diabetics, and salivary 8‐OHdG alone or in combination with MDA and hsCRP for systemically healthy persons, could potentially serve as non‐invasive screening marker(s) of periodontitis.

The aim of this study was to determine the prevalence of dissociative identity disorder (DID) and other dissociative disorders among adolescent psychiatric outpatients. A total of 116 consecutive outpatients between 11 and 17 years of age who were admitted to the child and adolescent psychiatry clinic of a university hospital for the 1st time were evaluated using the Adolescent Dissociative Experiences Scale, adolescent version of the Child Symptom Inventory-4, Childhood Trauma Questionnaire, and McMaster Family Assessment Device. All patients were invited for an interview with the Structured Clinical Interview for DSM-IV Dissociative Disorders (SCID-D) administered by 2 senior psychiatrists in a blind fashion. There was excellent interrater reliability between the 2 clinicians on SCID-D diagnoses and scores. Among 73 participants, 33 (45.2%) had a dissociative disorder: 12 (16.4%) had DID, and 21 (28.8%) had dissociative disorder not otherwise specified. There was no difference in gender distribution, childhood trauma, or family dysfunction scores between the dissociative and nondissociative groups. Childhood emotional abuse and family dysfunction correlated with self-reported dissociation. Of the dissociative adolescents, 93.9% had an additional psychiatric disorder. Among them, only separation anxiety disorder was significantly more prevalent than in controls. Although originally designed for adults, the SCID-D is promising for diagnosing dissociative disorders in adolescents, its modest congruence with self-rated dissociation and lack of relationship between diagnosis and childhood trauma and family dysfunction suggest that the prevalence rates obtained with this instrument originally designed for adults must be replicated. The introduction of diagnostic criteria for adolescent DID in revised versions of the Diagnostic and Statistical Manual of Mental Disorders, Fifth Edition, would refine the assessment of dissociative disorders in this age group.

Background ADAMTS (a disintegrin‐like and metalloproteinase with thrombospondin) are a family of proteinases that are structurally similar to the family of matrix metalloproteinases with critical roles in damage and repair of the extracellular matrix. Their functions are closely related to inflammation, hypoxia, and vascularization. Our aim was to determine levels of ADAMTS‐1 in gingival crevicular fluid (GCF) in patients with advanced periodontal diseases and identify their association with hypoxia‐inducible factor‐1alpha (HIF‐1α), vascular endothelial growth factor (VEGF‐A), and clinical parameters of periodontitis. Methods The study consisted of three groups: healthy individuals (control; n = 20), generalized chronic periodontitis (CP; n = 21), and generalized aggressive periodontitis (GAgP; n = 20). Clinical parameters were measured. Levels of ADAMTS‐1, VEGF‐A, and HIF‐1α in GCF and serum were quantified by enzyme‐linked immunosorbent assay (ELISA) and reported as total amounts and concentration. Results ADAMTS‐1 total amount in GCF were significantly higher in patients with CP and GAgP compared with healthy individuals (P < 0.05). HIF‐1α total amount in GCF were also higher in periodontitis groups compared with the control group (P < 0.05). GCF total VEGF‐A content was significantly higher in the GAgP group compared with the CP and the controls (respectively; P = 0.023, P = 0.003). There was a significant correlation between ADAMTS‐1, VEGF‐A, and HIF‐1α levels in the GCF and clinical periodontal parameters (probing depth [PD], bleeding on probing [BOP], and clinical attachment loss (CAL); P < 0.05). Conclusion ADAMTS‐1 may play a role in advanced periodontal disease pathogenesis in correlation with tissue hypoxia and vascularization.

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