High sensitive and selective electrochemical biosensor: Label-free detection of human norovirus using affinity peptide as molecular binder

Hwang, Hye Jin; Ryu, Myung Yi; Park, Chan Young; Ahn, Jun Ki; Park, Hyun Gyu; Choi, Changsun; Ha, Sang‐Do; Park, Tae Jung; Park, Jong Pil

doi:10.1016/j.bios.2016.08.031

Cited by 135 publications

(62 citation statements)

References 38 publications

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“…A highly sensitive impedimetric biosensor for detecting human norovirus by assembling a thiolated affinity peptide on a gold electrode was also developed [52]. This impedimetric genosensing approach showed a linear quantitative response to a wide range of norovirus concentrations (10–10 7 copies mL −1 ) and LODs of 99.8 nM for recombinant noroviral capsid proteins (rP2) and 7.8 copies mL −1 for real norovirus from diarrheal patients.…”

Section: Electrochemical Genosensing Of Circulating Biomarkersmentioning

confidence: 99%

Electrochemical Genosensing of Circulating Biomarkers

Campuzano

Yáñez‐Sedeño

Pingarrón

2017

Sensors

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Management and prognosis of diseases requires the measurement in non- or minimally invasively collected samples of specific circulating biomarkers, consisting of any measurable or observable factors in patients that indicate normal or disease-related biological processes or responses to therapy. Therefore, on-site, fast and accurate determination of these low abundance circulating biomarkers in scarcely treated body fluids is of great interest for health monitoring and biological applications. In this field, electrochemical DNA sensors (or genosensors) have demonstrated to be interesting alternatives to more complex conventional strategies. Currently, electrochemical genosensors are considered very promising analytical tools for this purpose due to their fast response, low cost, high sensitivity, compatibility with microfabrication technology and simple operation mode which makes them compatible with point-of-care (POC) testing. In this review, the relevance and current challenges of the determination of circulating biomarkers related to relevant diseases (cancer, bacterial and viral infections and neurodegenerative diseases) are briefly discussed. An overview of the electrochemical nucleic acid–based strategies developed in the last five years for this purpose is given to show to both familiar and non-expert readers the great potential of these methodologies for circulating biomarker determination. After highlighting the main features of the reported electrochemical genosensing strategies through the critical discussion of selected examples, a conclusions section points out the still existing challenges and future directions in this field.

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Section: Electrochemical Genosensing Of Circulating Biomarkersmentioning

confidence: 99%

Electrochemical Genosensing of Circulating Biomarkers

Campuzano

Yáñez‐Sedeño

Pingarrón

2017

Sensors

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“…Norovirus exposure occurs mainly from contaminated water or polluted food, and infection can rapidly spread from person to person through fecal‐oral routes. Infection with norovirus often occurs with relatively low amounts of virus, as low as 1×10 2 copies mL −1 …”

Section: Viral Infectionsmentioning

confidence: 99%

“…Hwang et al . developed highly sensitive EIS biosensor for detecting human norovirus by employing a thiolated affinity peptide (selected by phase display technique) on a gold sensor (Figure ).…”

Section: Viral Infectionsmentioning

confidence: 99%

Electrochemical Biosensing for the Diagnosis of Viral Infections and Tropical Diseases

2017

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Rapid and reliable diagnosis of viral infections and tropical diseases and timely initiation of appropriate treatment are essential for their successful clinical management. This Review summarizes some basic concepts regarding the main viral infection and tropical diseases to which society is facing today and the conventional methods available for their detection. The tremendous potential offered by electrochemical affinity biosensors to address this important challenge, meeting the required demands for viral infections and tropical diseases diagnosis, is clearly stated by discussing challenges, opportunities, implementation, and application of selected examples focused on the determination of specific biomarkers of different molecular (genetic, regulatory, and functional) levels. The highlighted approaches demonstrate the use of a plethora of specific receptors and assay formats and a great diversity of attractive electrochemical approaches in this rapidly advancing and highly interesting field.

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“…The limitations of these methods are large material consumption, demanding sample pretreatments and expensive equipments. While, the electrochemical methods have been developed as one of the efficient technique due to their simplicity, portability and low cost method [10]. In the recent years, the miniaturization, fast response time, cheap and easy process of analytical systems made an interesting research section for their development [11].…”

Section: Introductionmentioning

confidence: 99%

Rapid Determination of Phenolic Compounds in Water Samples: Development of a Paper‐based Nanobiosensor Modified with Functionalized Silica Nanoparticles and Potato Tissue

et al. 2019

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In this study, we present a fast, simple, low‐cost and disposable method for determination of phenolic content in water samples, using a paper based polyphenol oxidase biosensor. The propylamine functionalized silica nanoparticles was dropped onto a paper sheet. After drying at room temperature, the potato tissue extract including polyphenol oxidase was immobilized on the paper via physical and chemical adsorption. The modified paper was placed on the top of the graphite screen printed electrode. To construct of an electrochemical nanobiosensor, the electrochemical behavior of the modified electrode in different steps was investigated by cyclic voltammetry and electrochemical impedance spectroscopy methods. After being optimized the effective parameters, the changes in the biosensor electrochemical response vs. to the different concentrations of the substrate (phenol solution) were monitored by differential pulse voltammetry and amperometry methods. The linear relationships for phenol detection were obtained in the concentration ranges of 0.01–160 μM and 0.1–300 μM with a detection limit of 0.007 μM and 0.042 μM with DPV and amperometry methods, respectively. This method was successfully used in the voltammetric determination of the phenol content in the real samples, like the river water and the wastewater of wood factory.

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High sensitive and selective electrochemical biosensor: Label-free detection of human norovirus using affinity peptide as molecular binder

Cited by 135 publications

References 38 publications

Electrochemical Genosensing of Circulating Biomarkers

Electrochemical Genosensing of Circulating Biomarkers

Electrochemical Biosensing for the Diagnosis of Viral Infections and Tropical Diseases

Rapid Determination of Phenolic Compounds in Water Samples: Development of a Paper‐based Nanobiosensor Modified with Functionalized Silica Nanoparticles and Potato Tissue

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