Electrochemical Biosensing for the Diagnosis of Viral Infections and Tropical Diseases

Campuzano, Susana; Yáñez‐Sedeño, Paloma; Pingarrón, José M.

doi:10.1002/celc.201600805

Cited by 32 publications

(11 citation statements)

References 136 publications

(180 reference statements)

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“…[1] The flu viruses also mutate to infect human beings by antigenic shift. [6] www.advancedsciencenews.com www.advhealthmat.de Nd 3+ ions that can minimize heat-induced dehybridization or oligo damages while improving upconversion efficiency in aqueous environment, core-shell NaGdF 4 :Yb/Er@NaGdF 4 :Yb/ Nd UCNPs (csUCNPs) were adopted as the energy donor for sandwich detection. The conventional enzyme-linked immunosorbent assay (ELISA) and real-time polymerase chain reactions (qPCR) are well-recognized tools for the detection of flu viruses by either detecting the antigens or oligonucleotides (oligos) of the viruses.…”

Section: Introductionmentioning

confidence: 99%

Upconversion Luminescence Sandwich Assay For Detection of Influenza H7 Subtype

Tsang

Wong

Tsoi

et al. 2019

Adv Healthcare Materials

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Those sensors increase the limits of detection (LOD) by using signal amplifiers but the hybridization times are usually long. [7] On the other hand, colorimetric sensor produce visible color changes upon the presence of the virus genes. The simplicity in design and readout of these sensors had attracted substantial attention. Gold nanoparticles (AuNPs) are one of the promising candidates for such detection strategy because of their high extinction coefficient and aggregation-dependent color change. [8,9] Despite the advantages, AuNPs are surface-sensitive and may induce undesired aggregations due to high ionic strength and concentration of ions, resulting in false-positive signals.Apart from those approaches, optical detection has drawn substantial attention because of their simplicity, sensitiveness, and multiplexity. [10,11] It is the fact that organic dyes are widely used in conventional qPCR assays via downconversion processes. [4,12,13] However, the dyes demonstrate undesirable emissive properties such as poor photostability, broad emission band, and small Stokes shift. [14] Therefore, they were usually employed as quenchers in nanoparticle-based detection systems. [15][16][17] High energy excited quantum dots (QDs) are another representative fluorophores in downconverting assays. [18][19][20] The excitation may induce background fluorescence and broad emission peaks, contributing to severe cross-talking problem. To address these limitations, nearinfrared (NIR) triggered upconversion luminescence (UCL) in lanthanide-doped upconversion nanoparticles (UCNPs) is very promising in biodetection. They not only display large anti-Stokes shift [21,22] but also pose minimal damage to the gene oligos. In addition, the long-lived UCL signal is easily distinguished from the short-lived background signal. As a result, UCNPs were used to detect different oligos and analytes. [23][24][25][26][27][28] The assays involved highly distance-dependent luminescence resonance energy transfer (LRET) between energy donor and acceptor, in which the luminescent intensity changes with the separation between the energy pair. [29] In this work, green emitting UCNPs and AuNPs are selected as the LRET pair owing to their well-matched emission and extinction spectrum, while AuNPs exhibit excellent extinction coefficient and photostability. The size of the nanoparticles played important roles in hybridization [30] because AuNPs demonstrate sizedependent absorption property and it can alter the stability of the hybridized DNA duplex. In addition, the size ratios of the UCNPs and AuNPs affect the formation of UCNPs-AuNPs hybrid structures. Taking advantages of 808 nm excitation of With large anti-Stokes shifts and background-free signals, upconversion luminescent (UCL) screening assays have been a promising method to reduce the transmission of influenza epidemic, which can critically alleviate the disease burden and extra annual deaths. In this work, a luminescent resonance energy transfer sandwich assay is developed, which utilizes core-shell...

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Section: Introductionmentioning

confidence: 99%

Upconversion Luminescence Sandwich Assay For Detection of Influenza H7 Subtype

Tsang

Wong

Tsoi

et al. 2019

Adv Healthcare Materials

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“…There are also noble metal-based materials like silver, gold [89,90], platinum, zinc, cadmium, and others [91]. Moreover, every listed material can undergo multiple methods of surface modification, not only with nanoparticles [91,92,93,94,95,96,97], but hybrids and biological materials, like aptamers [98,99,100], sialic acid [88] or its derivatives [101], composites [84,85,102], nanohybrids [103] and dyes [86].…”

Section: Novel Detection Methods Of Influenza Virusmentioning

confidence: 99%

Detection Methods of Human and Animal Influenza Virus—Current Trends

Dziąbowska¹,

Czaczyk²,

Nidzworski³

2018

Biosensors

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The basic affairs connected to the influenza virus were reviewed in the article, highlighting the newest trends in its diagnostic methods. Awareness of the threat of influenza arises from its ability to spread and cause a pandemic. The undiagnosed and untreated viral infection can have a fatal effect on humans. Thus, the early detection seems pivotal for an accurate treatment, when vaccines and other contemporary prevention methods are not faultless. Public health is being attacked with influenza containing new genes from a genetic assortment between animals and humankind. Unfortunately, the population does not have immunity for mutant genes and is attacked in every viral outbreak season. For these reasons, fast and accurate devices are in high demand. As currently used methods like Rapid Influenza Diagnostic Tests lack specificity, time and cost-savings, new methods are being developed. In the article, various novel detection methods, such as electrical and optical were compared. Different viral elements used as detection targets and analysis parameters, such as sensitivity and specificity, were presented and discussed.

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“…The EC TN detection method offers portability (electrode size miniaturized through nano/microtechnology), modularity (can be integrated with a smartphone or arduino-based device), minimal requirements for sample volume, and short measurement time [28,29,30]. Usually, EC TN detection can be achieved by such methods as voltammetric, potentiometric, conductometric, amperometric, and impedimetric techniques [31,32,33]. The potential and current change by a redox material–mediated reaction between a target TN and a bioprobe can be used to determine the TN concentration accurately [34].…”

Section: Ec-based Tn Detection System With Nanostructurementioning

confidence: 99%

Development of the Troponin Detection System Based on the Nanostructure

et al. 2019

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During the last 30 years, the World Health Organization (WHO) reported a gradual increase in the number of patients with cardiovascular disease (CVD), not only in developed but also in developing countries. In particular, acute myocardial infarction (AMI) is one of the severe CVDs because of the high death rate, damage to the body, and various complications. During these harmful effects, rapid diagnosis of AMI is key for saving patients with CVD in an emergency. The prompt diagnosis and proper treatment of patients with AMI are important to increase the survival rate of these patients. To treat patients with AMI quickly, detection of a CVD biomarker at an ultra-low concentration is essential. Cardiac troponins (cTNs), cardiac myoglobin (cMB), and creatine kinase MB are typical biomarkers for AMI detection. An increase in the levels of those biomarkers in blood implies damage to cardiomyocytes and thus is related to AMI progression. In particular, cTNs are regarded as a gold standard biomarker for AMI diagnosis. The conventional TN detection system for detection of AMI requires long measurement time and is labor-intensive and tedious. Therefore, the demand for sensitive and selective TN detection techniques is increasing at present. To meet this demand, several approaches and methods have been applied to develop a TN detection system based on a nanostructure. In the present review, the authors reviewed recent advances in TN biosensors with a focus on four detection systems: (1) An electrochemical (EC) TN nanobiosensor, (2) field effect transistor (FET)-based TN nanobiosensor, (3) surface plasmon resonance (SPR)-based TN nanobiosensor and (4) surface enhanced Raman spectroscopy (SERS)-based TN nanobiosensor.

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Electrochemical Biosensing for the Diagnosis of Viral Infections and Tropical Diseases

Cited by 32 publications

References 136 publications

Upconversion Luminescence Sandwich Assay For Detection of Influenza H7 Subtype

Upconversion Luminescence Sandwich Assay For Detection of Influenza H7 Subtype

Detection Methods of Human and Animal Influenza Virus—Current Trends

Development of the Troponin Detection System Based on the Nanostructure

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