High quality draft genome sequence of Janthinobacterium psychrotolerans sp. nov., isolated from a frozen freshwater pond

Abstract

Strain S3-2T, isolated from sediment of a frozen freshwater pond, shares 99% 16S rRNA gene sequence identity with strains of the genus Janthinobacterium. Strain S3-2T is a facultative anaerobe that lacks the ability to produce violacein but shows antibiotic resistance, psychrotolerance, incomplete denitrification, and fermentation. The draft genome of strain S3-2T has a size of ~5.8 Mbp and contains 5,297 genes, including 115 RNA genes. Based on the phenotypic properties of the strain, the low in silico DNA-DNA hybridization (DDH) values with related genomes (<35%), and the low whole genome-based average nucleotide identity (ANI) (<86%) with other strains within the genus Janthinobacterium, we propose that strain S3-2T is the type strain (= DSM 102223 = LMG 29653) of a new species within this genus. We propose the name Janthinobacterium psychrotolerans sp. nov. to emphasize the capability of the strain to grow at low temperatures.Electronic supplementary materialThe online version of this article (doi:10.1186/s40793-017-0230-x) contains supplementary material, which is available to authorized users.

Strategies for high-altitude adaptation revealed from high-quality draft genome of non-violacein producing Janthinobacterium lividum ERGS5:01

“…Both the values qualify above the cut-off value for species boundary [ 33 , 34 ], and hence the results were consistent with the MLSA clustering of strain ERGS5:01 with PAMC 25724. In a recent study, strain PAMC 25724 has been reported as the strain of the species J. lividum [ 35 ] and is a validly published species with the availability of culture at Polar and Alpine Microbial Collection with accession number 25724. In another study, strains HH100, HH102, HH103, HH104, HH106, HH107, and 5059B had shown to be clustered together in proximity to J. lividum DSM 1522 [ 6 ].…”

“…Comparative genomic study was performed to reveal the genomic diversity across the genus Janthinobacterium . Genome data included J. agaricidamnosum DSM9628 (HG322949, [ 36 ]); J. psychotolerans S3–2 (LOCQ00000000, [ 35 ]) and J. lividum with multiple strains of Marseille (CP000269; [ 37 ]); Ant5–2 (LNCE00000000, [ 38 ]); PAMC 25724 (AHHB00000000, [ 2 ]; CG3 (APFF00000000, [ 39 ]); HH01 (NZ_AMWD00000000, [ 40 ]); RIT308 (JFYR00000000, [ 41 ]); MTR (JRRH00000000, [ 42 ]);RA13 (JQNP01000001, [ 43 ]); KBS0711 (LBCO00000000, [ 44 ]); CG23_2 (CYSS00000000, [ 45 ]); DSM1522 (LRHW00000000), MP5059B (LRHX00000000), HH100 (LRHY00000000), HH102 (LRHZ00000000), HH103 (LRIA00000000), HH104 (LRIB00000000), HH106 (LRIC00000000), and HH107 (LRID00000000) [ 4 ]. Few other genome sequence were retrieved from Gold database or IMG [ 22 , 23 ]: J. lividum strain NFR18 (FPKH00000000); Janthinobacterium sp.…”

Complete Genome Sequence of a Psychrotolerant Denitrifying Bacterium, Janthinobacterium svalbardensis PAMC 27463

“…Unexpectedly, the vioABCDE operon, which contains the genes responsible for the synthesis of the violacein pigment, was not found in the genome, but some species are not considered to have this gene set ( 9 ). The genome shows several enzymes involved in denitrification processes.…”

Variation of Bacterial and Archaeal Community Structures in a Full-Scale Constructed Wetlands for Wastewater Treatment

“…Pseudomonas was responsible for chronic infection [ 47 ] and was the most common bacteria in the soil [ 48 ], and this may explain why the relative abundances of Pseudomonas in dry soil sample were higher than those in water and wet soil samples. Janthinobacterium may cause a soft rot disease of Agaricus bisporus [ 49 ] and were isolated from both water [ 50 ] and soil [ 51 ]; however, there were no such genus in the soil samples of this study.…”