High Prevalence of &lt;i&gt;Campylobacter ureolyticus&lt;/i&gt; in Stool Specimens of Children with Diarrhea in Japan

Hatanaka, Noritoshi; Shimizu, Akinori; Somroop, Srinuan; Li, Yiming; Asakura, Masahiro; Nagita, Akira; Awasthi, Sharda Prasad; Hinenoya, Atsushi; Yamasaki, Shinji

doi:10.7883/yoken.jjid.2016.428

Cited by 15 publications

(12 citation statements)

References 10 publications

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“…Diarrheal disease remains an important medical conundrum and causes significant morbidity and mortality in developing countries ( Allos, 2001 ; Guerrant et al, 2001 ; Samosornsuk et al, 2015 ; Nohra et al, 2016 ; Hatanaka et al, 2017 ; Ramonaite et al, 2017 ; Wozniak-Biel et al, 2017 ). Campylobacter , Salmonella , Shigella , Diarrheagenic E. coli (DEC), and Vibrio parahaemolyticus are the common pathogens for diarrhea.…”

Section: Introductionmentioning

confidence: 99%

Prevalence and Molecular Characterization of Campylobacter spp. Isolated from Patients with Diarrhea in Shunyi, Beijing

Liu

Zhang

et al. 2018

Front. Microbiol.

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Bacterial pathogens have been confirmed as the major cause of acute diarrhea among outpatients in China. In this study, 370 stool samples from the patients aged from 15 to 87 years old with diarrhea were collected over 12 months (from May 2016 to April 2017) in two hospitals in Shunyi, Beijing. Bacterial isolation was performed for the common enteric pathogens: Campylobacter, Salmonella, Shigella, Diarrheagenic Escherichia coli, and Vibrio parahaemolyticus for 370 samples. The filtration method was used for the Campylobacter isolation in this study. The prevalence and molecular characterization of the Campylobacter were investigated. The isolation ratio for Campylobacter, Salmonella, Shigella, Diarrheagenic E. coli, and V. parahaemolyticus was 7.0% (26/370), 6.2% (23/370), 0.3% (1/370), 7.3% (27/370), and 10.3% (38/370), respectively. Based on the isolation result, Campylobacter positive cases presented in almost every month of the whole year and the isolation ratio was the highest among the tested pathogens during October to March. There was no significant difference between genders of Campylobacter positive cases. More Campylobacter positive cases presented dehydration compared with those who were positive for Salmonella. Twenty-six Campylobacter isolates were obtained in this study and 24 of these were Campylobacter jejuni. The antibiotic susceptibility tests indicated that 83.3% (20/24) of the isolates exhibited resistance to three or more types of antibiotic. Twenty STs were identified for the 26 Campylobacter isolates and four novel STs were identified in this study. No clonal cluster was found among these isolates. This is the first study for Campylobacter isolated using the filtration method in China which indicated the Campylobacter infection might be seriously under-ascertained in the diarrheal patients in China.

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Section: Introductionmentioning

confidence: 99%

Prevalence and Molecular Characterization of Campylobacter spp. Isolated from Patients with Diarrhea in Shunyi, Beijing

Liu

Zhang

et al. 2018

Front. Microbiol.

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“…Our results showed that Prevotellaceae-NK3B31-group exhibited the greatest change in relative abundance before and after PRRSV infection. Campylobacter , an active intestinal pathogen, is considered a potential pathogen of diarrhea [ 57 ]. In the study, the relative abundance of gut Campylobacter in susceptible pigs was three folds as high as that in resistant pigs at 7 dpi, and the degree of diarrhea was significantly higher in susceptible pigs than in resistant pigs, suggesting that Campylobacter might be involved in the pathogenesis of PRRSV diarrhea.…”

Section: Discussionmentioning

confidence: 99%

Change of Gut Microbiota in PRRSV-Resistant Pigs and PRRSV-Susceptible Pigs from Tongcheng Pigs and Large White Pigs Crossed Population upon PRRSV Infection

Wang

Guan

et al. 2022

Animals

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Porcine Reproductive and Respiratory Syndrome (PRRS) is one of the serious infectious diseases that threatens the swine industry. Increasing evidence shows that gut microbiota plays an important role in regulating host immune responses to PRRS virus (PRRSV). The aim of this study was to investigate gut microbiota difference between PRRSV-resistant pigs and PRRSV-suspectable pigs derived from a Tongcheng pigs and Large White pigs crossed population. PRRSV infection induces an increase in the abundance and diversity of gut microbiota. Correlation analysis showed that 36 genera were correlated with viral loads or weight gain after PRRSV infection. Prevotellaceae-NK3B31-group, Christensenellaceae-R7-group, and Parabacteroides were highly correlated with both viral load and weight gain. Notably, the diversity and abundance of beneficial bacteria such as Prevotellaceae-NK3B31-group was high in resistant pigs, and the diversity and abundance of pathogenic bacteria such as Campylobacter and Desulfovibrio were high in susceptible pigs. Gut microbiota were significantly associated with immune function and growth performance, suggesting that these genera might be related to viremia, clinical symptoms, and disease resistance. Altogether, this study revealed the correlation of gut microbiota with PRRSV infection and gut microbiota interventions may provide an effective prevention against PRRSV infection.

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“…Most clinical laboratories do not regularly test for other Campylobacter spp. such as C. concisus and C. ureolyticus due to both the fastidious nature of the organisms, the requirement for an enriched H 2 environment in order for growth and both species being unable to grow on the selective media that is commonly used for C. jejuni and C. coli [ 16 , 20 , 33 ]. It is highly probable that due to these reasons, gastroenteritis caused by C. concisus and C. ureolyticus is highly underestimated.…”

Section: Discussionmentioning

confidence: 99%

“…In a 2 year study conducted in Denmark, Nielsen et al showed that C. concisus was prevalent in 26.1% of patients with gastroenteritis as compared to C. jejuni/coli detected in 31.9% of infected patients [ 15 ]. In the first report of C. ureolyticus in children with gastroenteritis in Japan, Hatanaka et al showed that C. ureolyticus was prevalent in 51.9% of the infected children as compared to C. jejuni/coli infecting 15.5% of the children [ 20 ]. In a study from Southern Ireland, C. ureolyticus was prevalent in 41% of diarrheal patients as compared to C. jejuni at 50.7% and C. coli at 5.7% [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

“…C. ureolyticus , like C. concisus , has been detected more frequently in diarrheal cases. A recent study from Hatanaka et al showed that C. ureolyticus was detected in 51.9% of diarrheal stool samples from children with gastroenteritis [ 20 ]. Similar studies conducted in Ireland and Chile have also implicated C. ureolyticus as an emerging gastrointestinal pathogen [ 21 – 23 ].…”

Section: Introductionmentioning

confidence: 99%

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Incidence of Campylobacter concisus and C. ureolyticus in traveler’s diarrhea cases and asymptomatic controls in Nepal and Thailand

et al. 2017

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Background Campylobacter concisus and C. ureolyticus have emerged in recent years as being associated with acute and prolonged gastroenteritis and implicated in the development of inflammatory bowel diseases. However, there are limited data on the prevalence of these microorganisms in Southeast Asia. In this study, 214 pathogen-negative stool samples after laboratory examination for common enteric pathogens to include C. jejuni and C. coli by culture from two case–control traveler’s diarrhea (TD) studies conducted in Thailand (cases = 26; controls = 30) and Nepal (cases = 83; controls = 75) respectively were assayed by PCR for the detection of Campylobacter 16S rRNA and two specific heat shock protein genes specific for C. concisus (cpn60) and C. ureolyticus (Hsp60) respectively.Results Campylobacter 16S rRNA was detected in 28.5% (61/214) of the pathogen-negative TD stool samples (CIWEC Travel Medicine Clinic, Kathmandu, Nepal: cases = 36, control = 14; Bamrungrad International Hospital, Bangkok, Thailand: cases = 9, controls = 2). C. consisus was identified significantly more often in TD cases in Nepal (28.9%; 24/83) as compared to controls (4%; 3/75) (OR = 9.76; 95% CI 2.80–34.02; P = 0.0003) while C. consisus was detected in only two cases (2/26; 7.7%) and none of the controls stool samples from Thailand. C. ureolyticus was detected in four cases (4.8%; 4/83) and four controls (5.3%; 4/75) and in one case (3.8%; 1/26) and one control (3.1%; 1/30) from Nepal and Thailand respectively. C. jejuni and C. coli were isolated in 18.3 and 3.4% of the cases and in 4.0 and 1.4% of the controls in stool samples from both Thailand and Nepal respectively while C. concisus nor C. ureolyticus were not tested for in these samples.ConclusionThese findings suggest that C. concisus potentially is a pathogen associated with TD in Nepal. To our knowledge, this is the first report of C. concisus and C. ureolyticus detected from traveler’s diarrhea cases from travelers to Nepal and Thailand.

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High Prevalence of <i>Campylobacter ureolyticus</i> in Stool Specimens of Children with Diarrhea in Japan

Cited by 15 publications

References 10 publications

Prevalence and Molecular Characterization of Campylobacter spp. Isolated from Patients with Diarrhea in Shunyi, Beijing

Prevalence and Molecular Characterization of Campylobacter spp. Isolated from Patients with Diarrhea in Shunyi, Beijing

Change of Gut Microbiota in PRRSV-Resistant Pigs and PRRSV-Susceptible Pigs from Tongcheng Pigs and Large White Pigs Crossed Population upon PRRSV Infection

Incidence of Campylobacter concisus and C. ureolyticus in traveler’s diarrhea cases and asymptomatic controls in Nepal and Thailand

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