High prevalence of Helicobacter pylori in saliva demonstrated by a novel PCR assay.

Abstract

Aims-To investigate the prevalence of Helicobacterpylori in the saliva ofpatients infected with this bacterium. Methods-A novel polymerase chain reaction (PCR) assay was developed to detect Hpylori in saliva and gastric biopsy specimens from patients undergoing endoscopy. Results-Our PCR assay amplified a 417 base pair fragment of DNA from all 21DNAs derived from H pylon clinical isolates but did not amplify DNA from 23 non-H pylon strains. Sixty three frozen gastric biopsy and 56 saliva specimens were tested. H pylon specific DNA was detected by PCR in all 39 culture positive biopsy specimens and was also identified from another seven biopsy specimens which were negative by culture but positive by histology. H pylon specific DNA was identified by PCR in saliva specimens from 30 (75%) of 40 patients with H pylori infection demonstrated by culture or histological examination, or both, and in three patients without H pylon infection in the stomach. Conclusion-The results indicate that the oral cavity harbours H pylon and may be the source of infection and transmission. (J Clin Pathol 1995;48:662-666)

Association of cagA+ Helicobacter pylori with Adenotonsillar Hypertrophy

“…Despite some published reports on epidemiology of the bacterium (Li et al 1995;MravakStipetic et al 1998;di Banaventura et al 2001;Cirak et al 2003;Morinaka et al 2003), mode of transmission and role of H. pylori detected from tonsil and adenoid tissues on its epidemiology have not been completely understood. There are contradictory data on the presence of H. pylori in these tissues.…”

“…This pathogen causes one of the most common chronic bacterial infection in humans and it is accepted to have a role in development of chronic gastritis and gastric ulcer (Forman et al 1991;Suerbaum and Michetti 2002). In addition to gastric specimens, detection of H. pylori from different regions such as oral region suggests that the bacterium may lead to a broad spectrum of clinical manifestations (Li et al 1995;Cammarota et al 1996;Mravak-Stipetic et al 1998;Jun et al 2005). The presence of H. pylori in oral samples may be a cause of the high incidence of H. pylori infection in the world (Unver et al 2001;Ozdek et al 2003;Morinaka et al 2003).…”

vacA genotypes in oral cavity and Helicobacter pylori seropositivity among adults without dyspepsia

“…The transmission mechanism is not well established but infected saliva or food contamination seems to be most probable (2). The H. pylori DNA has been detected in saliva and dental plaque from patients with gastroduodenal pathology (3)(4)(5)(6)(7). This fact strengthens the hypothesis that the oral cavity is a reservoir for H. pylori, it could be the source for gastric infection and re-infection post therapy as well as person-to-person transmission (3).…”

“…This fact strengthens the hypothesis that the oral cavity is a reservoir for H. pylori, it could be the source for gastric infection and re-infection post therapy as well as person-to-person transmission (3). Nevertheless, the role H. pylori plays in the oral cavity is controversial since the detection rate of the bacterium in the mouth is 0% to 100% (3)(4)(5)(6)(7)(8). Gastroesophagic reflux, deficient oral hygiene and frequent vomiting are conditions that may facilitate oral colonization and also the environment and life-style could be decisive for H. pylori infection (4).…”

Investigation of Helicobacter pylori in Laryngeal Papillomatosis

“…CagA protein is present in 60-70% of all strains of H. pylori that is associated with increasing the risk of cancer, developing of atrophic gastritis, forming the ulcer and deferring in recovery, inducting of expression of oncogenes and mutation in p53 gene, atherosclerosis, ischemic heart disease, coronary heart disease, producing of PGE2 and increasing the production of reactive forms of oxygen (5,(16)(17)(18)(19)(20)(21). In several studies, the presence of H. pylori has been confirmed in oral cavity, dental plaque ,saliva and its colonization in the nose and along with a number of diseases of the throat and larynx (21)(22)(23)(24). H. pylori causes increased proliferation and decreased apoptosis in the tissues of the larynx.…”