Investigation of Helicobacter pylori in Laryngeal Papillomatosis

Abstract

Background:Helicobacter pylori (HP) is a motile, gram negative, catalase and oxidase positive bacteria that produce strong urease. H. pylori has been recognized as an etiologic agent of gastritis, gastric ulcer, gastric adenocarcinoma and gastric MALT lamphoma. H. pylori infections have been correlated with many throat and larynx disease. More recently it is introduced as group 1 carcinogen. The existence of H. pylori within bouccal cavity and dental plaque has been reported. The nasal cavity can be colonized easily with H. pylori. Objectives: The aim of this Study is to report the result of an investigation of H. pylori genome within the larynx popilomatosis biopsy samples. Materials and Methods: 41 biopsy samples were provided from papillomatosis cases during therapeutic surgery. The biopsy samples were preserved at -80°C until use. The DNA extractions were achieved by the phenole-chloroform method. The cagA of H. pylori were amplified by specific primer sets. Results: H. pylori DNA were detected from 3 out of 41 (0.7%) papilloma samples. Conclusions: This result is in agree with another reports about correlation between laryngeal papilomatosis and H. pylori infections.