High-performance olfactory receptor-derived peptide sensor for trimethylamine detection based on Steglich esterification reaction and native chemical ligation connection

Wang, Zhi; Ma, Weichao; Wei, Jinyu; Lan, Kuibo; Yan, Shanchun; Chen, Ruibing; Qin, Guoxuan

doi:10.1016/j.bios.2021.113673

Cited by 24 publications

(25 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The ORP sensor fabricated by the flat-condition connection can detect TMA gas at 10 parts per quadrillion (ppq) (Figure 5a), which was similar to the reported ORP sensor for TMA detection. 24 The reason for the increased resistance when the ORP sensor recognizes the TMA gas was as follows. Three amino acids (CNQLSNLSFSDLC) in the ORP were binding sites that recognize TMA.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…The ORP can specifically recognize gaseous TMA and can be chemically connected to the SWCNTs. 24 The ORPs were kept in a refrigerator with a stable temperature of −20 °C. The ORPs were dissolved to 0.01 mg/L with distilled water to link the SWCNTs.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

“…18 Recently, by changing the physical connection to a chemical connection, the LOD of the fabricated ORP sensors for TMA gas was lowered to 0.01 ppt (the lowest LOD reported until now). 24 However, a TMA sensor with even better loading efficiency of ORPs to achieve a lower detection limit and higher performance is still highly desirable. Up to now, the reported research studies mainly focused on the strategy of achieving effective physical absorption or using a chemical reaction for covalent modification to improve the efficiency of the connection between the ORPs and the carrier materials.…”

mentioning

confidence: 99%

“…However, a TMA sensor with even better loading efficiency of ORPs to achieve a lower detection limit and higher performance is still highly desirable. Up to now, the reported research studies mainly focused on the strategy of achieving effective physical absorption or using a chemical reaction for covalent modification to improve the efficiency of the connection between the ORPs and the carrier materials. , Here we propose that the loading efficiency of ORPs could also be significantly enhanced by increasing the number of connection sites on the carrier materials during the ORP-connection process in order to improve the performance of not only TMA detection but also other gas sensors. In addition, with the increasing needs of sensors that can be functional at deformation conditions for wearable and portable applications, high-performance flexible gas sensors are necessary.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Ultrasensitive Flexible Olfactory Receptor-Derived Peptide Sensor for Trimethylamine Detection by the Bending Connection Method

Wang

Wei

et al. 2022

ACS Sens.

Self Cite

View full text Add to dashboard Cite

Trimethylamine (TMA) is a harmful gas that exists ubiquitously in the environment; therefore, the sensitive and specific monitoring of TMA is necessary. In this work, we prepared ultrasensitive flexible sensors for TMA detection based on single-walled carbon nanotubes (SWCNTs) and olfactory receptor-derived peptides (ORPs) on low-cost plastic substrates. A novel bending connection method was developed by intentionally bending the interdigitated electrodes with SWCNTs to form a three-dimensional structure during the ORP-connection process, leading to the exposure of more modification sites. The method showed ∼4.7-fold more effective connection amount of the ORPs to SWCNTs compared to the conventional flat-condition connection method. The flexible ORP-SWCNT sensors could significantly improve the limit of detection for gaseous TMA from the reported lowest limit of 10 parts per quadrillion (ppq) to 0.1 ppq. The flexible ORP sensors also exhibited excellent sensitivity to vaporized TMA standards and TMA generated by different kinds of foods under different bending conditions. The results showed that the bending connection method in this work was effective for ultrasensitive flexible ORP sensors and their associated applications.

show abstract

Section: ■ Results and Discussionmentioning

confidence: 99%

Section: ■ Experimental Sectionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Ultrasensitive Flexible Olfactory Receptor-Derived Peptide Sensor for Trimethylamine Detection by the Bending Connection Method

Wang

Wei

et al. 2022

ACS Sens.

Self Cite

View full text Add to dashboard Cite

show abstract

“…The molecular docking model showed that the three-dimensional structure of BdorOBP2 has a central hydrophobic pocket, exposing a variety of chemical groups connected with pheromone chemicals through hydrophobic, polar, electrostatic, and π-stacking interactions to form stable conformational changes. Olfactory receptor-derived peptides (ORPs) were chemically immobilized onto single-walled carbon nanotubes (SWCNTs) on interdigitated electrodes based on Steglich esterification reaction (SER) and native chemical ligation (NCL), which achieved high responsiveness and stability to TMA with a detection of limit down to 0.01 ppt [ 117 ]. This novel ORP sensor has potential applications for environmental safety, food quality control, and healthcare.…”

Section: Various Biomimetic Olfactory and Gustatory Biosensorsmentioning

confidence: 99%

Progress in the Development of Detection Strategies Based on Olfactory and Gustatory Biomimetic Biosensors

Chen

Tian

et al. 2022

Biosensors

View full text Add to dashboard Cite

The biomimetic olfactory and gustatory biosensing devices have broad applications in many fields, such as industry, security, and biomedicine. The development of these biosensors was inspired by the organization of biological olfactory and gustatory systems. In this review, we summarized the most recent advances in the development of detection strategies for chemical sensing based on olfactory and gustatory biomimetic biosensors. First, sensing mechanisms and principles of olfaction and gustation are briefly introduced. Then, different biomimetic sensing detection strategies are outlined based on different sensing devices functionalized with various molecular and cellular components originating from natural olfactory and gustatory systems. Thereafter, various biomimetic olfactory and gustatory biosensors are introduced in detail by classifying and summarizing the detection strategies based on different sensing devices. Finally, the future directions and challenges of biomimetic biosensing development are proposed and discussed.

show abstract

Artificial Olfactory Biohybrid System: An Evolving Sense of Smell

Qin

Wang

et al. 2022

Advanced Science

View full text Add to dashboard Cite

The olfactory system can detect and recognize tens of thousands of volatile organic compounds (VOCs) at low concentrations in complex environments. Bioelectronic nose (B-EN), which mimics olfactory systems, is becoming an emerging sensing technology for identifying VOCs with sensitivity and specificity. B-ENs integrate electronic sensors with bioreceptors and pattern recognition technologies to enable medical diagnosis, public security, environmental monitoring, and food safety. However, there is currently no commercially available B-EN on the market. Apart from the high selectivity and sensitivity necessary for volatile organic compound analysis, commercial B-ENs must overcome issues impacting sensor operation and other problems associated with odor localization. The emergence of nanotechnology has provided a novel research concept for addressing these problems. In this work, the structure and operational mechanisms of biomimetic olfactory systems are discussed, with an emphasis on the development and immobilization of materials. Various biosensor applications and current developments are reviewed. Challenges and opportunities for fulfilling the potential of artificial olfactory biohybrid systems in fundamental and practical research are investigated in greater depth.

show abstract

High-performance olfactory receptor-derived peptide sensor for trimethylamine detection based on Steglich esterification reaction and native chemical ligation connection

Cited by 24 publications

References 48 publications

Ultrasensitive Flexible Olfactory Receptor-Derived Peptide Sensor for Trimethylamine Detection by the Bending Connection Method

Ultrasensitive Flexible Olfactory Receptor-Derived Peptide Sensor for Trimethylamine Detection by the Bending Connection Method

Progress in the Development of Detection Strategies Based on Olfactory and Gustatory Biomimetic Biosensors

Artificial Olfactory Biohybrid System: An Evolving Sense of Smell

Contact Info

Product

Resources

About