2022
DOI: 10.1016/j.bios.2021.113673
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High-performance olfactory receptor-derived peptide sensor for trimethylamine detection based on Steglich esterification reaction and native chemical ligation connection

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Cited by 24 publications
(25 citation statements)
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“…The ORP sensor fabricated by the flat-condition connection can detect TMA gas at 10 parts per quadrillion (ppq) (Figure 5a), which was similar to the reported ORP sensor for TMA detection. 24 The reason for the increased resistance when the ORP sensor recognizes the TMA gas was as follows. Three amino acids (CNQLSNLSFSDLC) in the ORP were binding sites that recognize TMA.…”
Section: ■ Results and Discussionmentioning
confidence: 99%
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“…The ORP sensor fabricated by the flat-condition connection can detect TMA gas at 10 parts per quadrillion (ppq) (Figure 5a), which was similar to the reported ORP sensor for TMA detection. 24 The reason for the increased resistance when the ORP sensor recognizes the TMA gas was as follows. Three amino acids (CNQLSNLSFSDLC) in the ORP were binding sites that recognize TMA.…”
Section: ■ Results and Discussionmentioning
confidence: 99%
“…The ORP can specifically recognize gaseous TMA and can be chemically connected to the SWCNTs. 24 The ORPs were kept in a refrigerator with a stable temperature of −20 °C. The ORPs were dissolved to 0.01 mg/L with distilled water to link the SWCNTs.…”
Section: ■ Experimental Sectionmentioning
confidence: 99%
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“…The molecular docking model showed that the three-dimensional structure of BdorOBP2 has a central hydrophobic pocket, exposing a variety of chemical groups connected with pheromone chemicals through hydrophobic, polar, electrostatic, and π-stacking interactions to form stable conformational changes. Olfactory receptor-derived peptides (ORPs) were chemically immobilized onto single-walled carbon nanotubes (SWCNTs) on interdigitated electrodes based on Steglich esterification reaction (SER) and native chemical ligation (NCL), which achieved high responsiveness and stability to TMA with a detection of limit down to 0.01 ppt [ 117 ]. This novel ORP sensor has potential applications for environmental safety, food quality control, and healthcare.…”
Section: Various Biomimetic Olfactory and Gustatory Biosensorsmentioning
confidence: 99%