Ultrasensitive Flexible Olfactory Receptor-Derived Peptide Sensor for Trimethylamine Detection by the Bending Connection Method

Abstract

Trimethylamine (TMA) is a harmful gas that exists ubiquitously in the environment; therefore, the sensitive and specific monitoring of TMA is necessary. In this work, we prepared ultrasensitive flexible sensors for TMA detection based on single-walled carbon nanotubes (SWCNTs) and olfactory receptor-derived peptides (ORPs) on low-cost plastic substrates. A novel bending connection method was developed by intentionally bending the interdigitated electrodes with SWCNTs to form a three-dimensional structure during the ORP-connection process, leading to the exposure of more modification sites. The method showed ∼4.7-fold more effective connection amount of the ORPs to SWCNTs compared to the conventional flat-condition connection method. The flexible ORP-SWCNT sensors could significantly improve the limit of detection for gaseous TMA from the reported lowest limit of 10 parts per quadrillion (ppq) to 0.1 ppq. The flexible ORP sensors also exhibited excellent sensitivity to vaporized TMA standards and TMA generated by different kinds of foods under different bending conditions. The results showed that the bending connection method in this work was effective for ultrasensitive flexible ORP sensors and their associated applications.

Biomimetic Wearable Sensors: Emerging Combination of Intelligence and Electronics

“…[ 145 ] Nevertheless, traditional electronic nose devices demonstrate the disadvantages of large sizes, difficulties in portability, and limited application scenarios. In contrast, emerging wearable biomimetic olfactory sensors can be employed for the recognition of dangerous gases, [ 23 , 146 ] food quality identification [ 23 , 147 ] and medical diagnosis [ 124 , 148 ] and are preferred by people due to the advantages of portability and diverse application scenarios.…”

“…Reproduced with permission. [ 23b ] Copyright 2022, Elsevier. f) Effective connection of ORP increased by 3D bending structure and used for detecting trimethylamine content in food.…”

“…This integration can be realized via sensor arrays, sensor networks, or modular sensor integration, all of which demonstrate advantages such as collecting multiple sensory information for comprehensive processing, enabling data sharing and collaborative work among sensors via wireless communication, and allowing combination and replacement according to user needs, thus enhancing perceptual abilities. [ 7 , 22 ] Currently, with the enrichment of substrate and sample unit material functions, system circuits, and data processing algorithms, WASSs have also developed biomimetic senses that surpass traditional human senses in some aspects, for instance, biomimetic smell that can identify dangerous and toxic gases [ 23 ] and biomimetic vision that transcends the visual field of human eyes, [ 24 ] which is expected to become an evolving human sense. Therefore, WASSs have become the most attractive and representative class of biomimetic wearable sensing systems.…”

Investigating on sensing mechanism of MoS2-FET biosensors in response to proteins

“…Biosensing technology has become a rapidly developing research field and is widely applied in areas such as medical diagnosis, environmental monitoring, and food safety [1,2]. Conventional biosensing techniques include enzyme labeling, radioisotope, immunofluorescence, mass spectrometry, surface plasmon resonance (SPR), and electrochemical methods [3][4][5][6].…”

Sex Pheromone Receptor-Derived Peptide Biosensor for Efficient Monitoring of the Cotton Bollworm Helicoverpa armigera

“…armigera SPRs by SWISS-MODEL, predicted the BS of pheromone compounds by molecular docking, and designed 3 SPR-derived peptides (SPRPs) with potential binding capability. Next, the SPRPs were synthesized and linked to single-walled carbon nanotubes (SWCNTs) by the chemical connection method, and the SPRP sensors were constructed. ,− The responses of these SPRP sensors to different concentrations of pheromone compounds were tested using resistance change rates, and compared with other gas chemicals, the selectivity of the sensors was evaluated. Finally, the performance of the sensors for sensing the sex pheromone released by live H.…”