High-performance liquid chromatography with on-line coupled UV, mass spectrometric and biochemical detection for identification of acetylcholinesterase inhibitors from natural products

Ingkaninan, Kornkanok; Best, C.M. de; Heijden, Robert van der; Hofte, A.J.P.; Karabatak, Bahadir; Irth, H.; Tjaden, U.R.; Greef, J. van der; Verpoorte, Robert

doi:10.1016/s0021-9673(99)01292-3

Cited by 194 publications

(127 citation statements)

References 8 publications

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“…The analysis rate of the segmented flow method compares favorably with previously reported flow injection AchE assays [26,27,29]. The speed of flow injection methods was limited by the need to inject individual samples or additional separation steps when assay buffer was not directly compatible with ESI-MS.…”

Section: Segmented Flow Esi-ms Analysis For Rapid Screeningmentioning

confidence: 63%

“…It may not always be possible to design assays that allow direct injection into an ESI source. Current flow injection systems can utilize in-line solid-phase extraction at high flow rates so their throughput is minimally impacted by this necessity [26]. Therefore, for the segmented flow approach to be as versatile as the flow injection approach for HTS, it is necessary to develop systems that allow compatible solid-phase extraction or similar sample preparation steps that are high-throughput.…”

Section: Overall Throughputmentioning

confidence: 99%

“…It has been demonstrated that AchE assays can be performed using flow-injection ESI-MS [26] and HPLC-MS [27] to directly detect substrate and/or product of the reaction. Throughput of 0.2 Hz with 1 to 5 L of sample consumption was possible when using an automated sampling and injection system [27].…”

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confidence: 99%

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Rapid and label-free screening of enzyme inhibitors using segmented flow electrospray ionization mass spectrometry

Pei

Kennedy

2010

J. Am. Soc. Mass Spectrom.

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Electrospray ionization mass spectrometry (ESI-MS) is an attractive analytical tool for high-throughput screening because of its rapid scan time and ability to detect compounds without need for labels. Impediments to the use of ESI-MS for screening have been the relatively large sample consumed and slow sample introduction rates associated with commonly used flow injection analysis. We have previously shown that by segmenting nanoliter plugs of sample with air, an array of discrete samples can be delivered to a platinum-coated emitter tip for ESI-MS analysis with throughput as high as 0.8 Hz and carry-over between samples less than 0.1%. This method was applied to screening for inhibitors of acetylcholinesterase as a demonstration of the potential of segmented flow ESI-MS for such applications. Each enzyme assay consumed 10 nL of sample. At 1 L/min infusion rate, 102 samples were analyzed, corresponding to a 0.65 Hz sample analysis rate. Linear quantification of choline was achieved from 200 M to 10 mM using this method and Z= values were over 0.8 for the assay. Detailed pharmacologic dose-response curves of selected inhibitors were also measured in high-throughput fashion to validate the method. (J Am Soc

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Section: Segmented Flow Esi-ms Analysis For Rapid Screeningmentioning

confidence: 63%

Section: Overall Throughputmentioning

confidence: 99%

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Rapid and label-free screening of enzyme inhibitors using segmented flow electrospray ionization mass spectrometry

Pei

Kennedy

2010

J. Am. Soc. Mass Spectrom.

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“…Such biochemical assays are of more interest to the pharmaceutical industry as many drugs target enzymes as part of their mode of action (van Elswijk and Irth 2003;Ingkaninan et al 2000;Schenk et al 2003;Schobel et al 2001). Within our group in cooperation with the Department of Toxicology of Wageningen University, we have developed an on-line HPLC system for detection of Cytochrome P450 (CYP450) 1A2 inhibitors.…”

Section: High-resolution Screeningmentioning

confidence: 99%

Recent developments in the rapid analysis of plants and tracking their bioactive constituents

et al. 2009

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“…The assay for measuring acetylcholinesterase activity was based from the assay described by Ellman et al (1961) and Ingkaninan et al (2000) with modifications. Briefly, 125 μL of 3 mM DTNB, 50 μL of sodium phosphate buffer (pH 8.0), 25 μL of the sample dissolved in DMSO and 25 μL of 0.5 U/mL acetylcholinesterase were added in a 96-well microplate and incubated for 15 min at 25°C.…”

Section: Acetylcholinesterase Enzyme Inhibitory Activitymentioning

confidence: 99%

Anti-oxidant and anti-acetylcholinesterase activities of Zygophyllum album

Kchaou

Salah

Mhiri

et al. 2015

Bangladesh J Pharmacol

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The present study was carried out to explore the anti-acetylcholinesterase activity and anti-oxidant effect as well as content of phenolic compounds of various extracts from Zygophyllum album. It was observed that dichloromethane and ethanol extracts were endowed with a powerful anti-acetylcholinesterase activity with IC50 values of 40 and 58 µg/mL, respectively. These two extracts exhibited the highest DPPH radical scavenging activity (IC50=0.2 mg/mL), the highest total anti-oxidant capacity (185.2 and 222.4 mg vitamin E/mg extract, respectively), the highest reducing power effect, and the highest β-carotene bleaching capacity. The findings showed that the extents of anti-acetylcholinesterase activity of Z. album extracts as well as their anti-oxidant capacity are in accordance with their phenolic contents. Hence, Z. album leaves would be useful against Alzheimer's disease. VIDEO CLIP<a href="https://www.youtube.com/v/M03zi4Mzh6k">Determination of flavonoids content:</a> 2 min 50 sec

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High-performance liquid chromatography with on-line coupled UV, mass spectrometric and biochemical detection for identification of acetylcholinesterase inhibitors from natural products

Cited by 194 publications

References 8 publications

Rapid and label-free screening of enzyme inhibitors using segmented flow electrospray ionization mass spectrometry

Rapid and label-free screening of enzyme inhibitors using segmented flow electrospray ionization mass spectrometry

Recent developments in the rapid analysis of plants and tracking their bioactive constituents

Anti-oxidant and anti-acetylcholinesterase activities of <i>Zygophyllum album</i>

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