High-performance liquid chromatography with nuclear magnetic resonance detection—A method for quantification of α- and γ-linolenic acids in their mixtures with free fatty acids

Sýkora, Ján; Bernášek, Prokop; Zarevúcká, Marie; Kurfürst, Milan; Sovová, Helena; Schraml, Jan

doi:10.1016/j.chroma.2006.11.041

Cited by 17 publications

(12 citation statements)

References 4 publications

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“…On the contrary, those fatty acids were distinguished, at least in the form of free acids, with the use of the LC-NMR method. These results were in agreement with those obtained by Sýkora et al in the analysis of naturally occuring mixtures of FFAs by LC-NMR [333]. When co-elution threatens, HPLC can be accomplished by using structure sensitive detection assuming that the co-eluting compounds differ in the number of double bonds, their positions and the length of the chain.…”

Section: Food Lipidssupporting

confidence: 93%

Recent trends in the advanced analysis of bioactive fatty acids

Ruiz-Rodrı́guez

Reglero

Ibáñez

2010

Journal of Pharmaceutical and Biomedical Analysis

120

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The consumption of dietary fats have been long associated to chronic diseases such as obesity, diabetes, cancer, arthritis, asthma, and cardiovascular disease; although some controversy still exists in the role of dietary fats in human health, certain fats have demonstrated their positive effect in the modulation of abnormal fatty acid and eicosanoid metabolism, both of them associated to chronic diseases. Among the different fats, some fatty acids can be used as functional ingredients such as alpha-linolenic acid (ALA), arachidonic acid (AA), eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), gamma-linolenic acid (GLA), stearidonic acid (STA) and conjugated linoleic acid (CLA), among others. The present review is focused on recent developments in FAs analysis, covering sample preparation methods such as extraction, fractionation and derivatization as well as new advances in chromatographic methods such as GC and HPLC. Special attention is paid to trans fatty acids due its increasing interest for the food industry.

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Section: Food Lipidssupporting

confidence: 93%

Recent trends in the advanced analysis of bioactive fatty acids

Ruiz-Rodrı́guez

Reglero

Ibáñez

2010

Journal of Pharmaceutical and Biomedical Analysis

120

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“…The separation started at 10% and ended at 90% of CDCl 3 in 100 minutes. Other chromatographic parameters were adjusted to the requirements of the quantitative LC-NMR analysis [26], for example, flow rate 0.5 mL/min (for details see the Supplementary Material available online at http://dx.doi.org/10.1155/2013/526818). …”

Section: Resultsmentioning

confidence: 99%

“…For the purpose of simultaneous analysis of free and conjugated sterols in natural extracts, we have extended previously published LC-NMR method originally developed for the analysis of free fatty acids in natural oils [26]. We have also developed fast composition screening method using SEC-NMR technique.…”

Section: Introductionmentioning

confidence: 99%

LC-NMR Technique in the Analysis of Phytosterols in Natural Extracts

Horník

Sajfrtová

Karban

et al. 2013

Journal of Analytical Methods in Chemistry

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The ability of LC-NMR to detect simultaneously free and conjugated phytosterols in natural extracts was tested. The advantages and disadvantages of a gradient HPLC-NMR method were compared to the fast composition screening using SEC-NMR method. Fractions of free and conjugated phytosterols were isolated and analyzed by isocratic HPLC-NMR methods. The results of qualitative and quantitative analyses were in a good agreement with the literature data.

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“…Furthermore, hyphenating NMR to HPLC is a very strong means to characterize the complex mixtures [117,118]. Sýkora et al developed LC-NMR method for quantification of ␣-and ␥-linolenic acids in mixtures, which could not be realized only with HPLC alone [119].…”

Section: Nmrmentioning

confidence: 99%