LC-NMR Technique in the Analysis of Phytosterols in Natural Extracts

Horník, Štěpán; Sajfrtová, Marie; Karban, Jindřich; Sýkora, Ján; Březinová, Anna; Wimmer, Zdeněk

doi:10.1155/2013/526818

Cited by 13 publications

(7 citation statements)

References 27 publications

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“…Quantification methods are numerous using GC of HPLC coupled to FID of MS (the most used methods). More recently, the interest of use of proton nuclear magnetic resonance (NMR) coupled to partial least square as a fast method to measure squalene and sterols vegetable oils, or liquid chromatography-NMR was reported [14,15].…”

Section: Introductionmentioning

confidence: 99%

Tetraploid Wheats: Valuable Source of Phytosterols and Phytostanols

Merah

Mouloungui

2019

Agronomy

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Phytosterols are known as healthy compounds obtained mainly from oilseed crops. Cereals were also studied for their sterols content. Few insights have been devoted to other tetraploid species than emmer and durum wheats. This work examined phytosterol and phytostanol content in seed of six tetraploid wheat species cultivated during two successive years under rainfed organic conditions in Auch (near Toulouse, France). Sterols (free and esterified sterols) were measured by gas-chromatography-flame ionisation detector. Mean value of sterols + stanols content was 99.5 mg 100 g−1 DW. The main sterol was β-sitosterol. Results showed a year effect on sterol content, whatever the wheat species. This could be explained by the differences in climatic conditions prevailing during plant cycle and grain filling. A large variability for sterols content was found between species and within each species. Emmer wheat revealed the lowest values for all sterols and stanols. Higher values of sterols were obtained in durum wheat. This work is the first report studying T. carthlicum, T. polonicum, T. turgidum, T. timopheevi. These species exhibited intermediate values of sterol contents between emmer and durum wheats. Wheat tetraploid species showed interesting levels of sterols and could serve as a great source of these healthy compounds mainly in Mediterranean region where they are consumed as wholegrain. Variation in climatic conditions could help to manage the level of these secondary metabolites.

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Section: Introductionmentioning

confidence: 99%

Tetraploid Wheats: Valuable Source of Phytosterols and Phytostanols

Merah

Mouloungui

2019

Agronomy

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“…The characteristic peaks of phytosterols like sitostanol, campesterol, avenasterol, cykloeukalenol and β-sitosterol reported by [13]. The spectrum showed one proton Sitosterol by [11] and [12] (Table 1).…”

Section: Resultsmentioning

confidence: 99%

β-SITOSTEROL ISOLATED FROM ETHYL ACETATE LEAF EXTRACT OF Cissus populnea AND THE ANTIMICROBIAL ACTIVITY

Danladi¹,

Ushie²,

Egwaikhide³

2023

JCSN

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β-Sitosterol was isolated from ethyl acetate leaf extract of Cissus populnea and characterized using H-NMR spectroscopy analysis. The antimicrobial activity of the leaf extract was also investigated in this study. Column chromatography was used to fractionate the ethyl extract, and thin layer chromatography was used to monitor the fractions. The result of the study showed that all isolates of Cissus populnea leaf inhibited or exhibited antibacterial and antifungal activities against tested microbes, with the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of Cissus populnea fractions against tested microbes or organisms ranging from 6.25 to 100g/ml. The majority of the minimum inhibitory concentration (MIC) activity levels were lower than the MBC levels. A lower MIC value indicates that fewer plant fragments (isolates) are needed to prevent organism growth.; thus, plants with lower MIC scores are more effective antimicrobial agents.

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“…The results obtained are reasonably consistent with the data in the literature. Horník et al 54 . documented that the predominant sterol in the nettle root extract was β‐sitosterol with a content of 81%, whereas Sajfrtová et al 11 .…”

Section: Resultsmentioning

confidence: 99%

Sterols and pentacyclic triterpenoids from nettle root: content and composition as affected by pressurized liquid extraction

et al. 2022

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BACKGROUND: Nettle is a medicinal plant rich in bioactive molecules. The composition of nettle leaves and stems has been extensively studied, whereas the root has been insufficiently investigated. Therefore, the present study aimed to optimize the parameters of advanced extraction technique, pressurized liquid extraction (PLE), for the lipid fraction of nettle root rich in triterpenoid derivatives and to compare the efficiency of isolation under optimal conditions with conventional Soxhlet extraction (SE). RESULTS:The PLE yields ranged from 0.39-1.63%, whereas the total content of triterpenoid derivatives ranged from 43.50-78.26 mg 100 g −1 , with nine sterols and three pentacyclic triterpenoids identified and quantified within a total range of 42.81-76.57 mg 100 g −1 and 0.69-1.68 mg 100 g −1 dried root, respectively. The most abundant sterol and pentacyclic triterpenoid were ⊎-sitosterol and ⊎-amyrin acetate, with mean values of 50.21 mg 100 g −1 and 0.56 mg 100 g −1 dried root.CONCLUSION: The optimal PLE conditions were 150 °C/5 min/four cycles and showed significantly better performance compared to SE (68 °C, 8 h), establishing an excellent technique for the isolation of the nettle root lipid fraction. Also, triterpenoid derivatives from nettle could be used as functional ingredients for the development of new foods and dietary supplements.

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LC-NMR Technique in the Analysis of Phytosterols in Natural Extracts

Cited by 13 publications

References 27 publications

Tetraploid Wheats: Valuable Source of Phytosterols and Phytostanols

Tetraploid Wheats: Valuable Source of Phytosterols and Phytostanols

β-SITOSTEROL ISOLATED FROM ETHYL ACETATE LEAF EXTRACT OF Cissus populnea AND THE ANTIMICROBIAL ACTIVITY

Sterols and pentacyclic triterpenoids from nettle root: content and composition as affected by pressurized liquid extraction

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