High Performance Liquid Chromatography (Hplc) for the Separation of Chiral Drugs

Structure Control of Polysaccharide Derivatives for Efficient Separation of Enantiomers by Chromatography

“…Specifically, direct chiral separation using chiral stationary phases (CSPs) for high-performance liquid chromatography (HPLC) has significantly evolved during the past few decades and is recognized as the most popular and reliable tool for both the analysis of enantiomer compositions and the preparation of pure enantiomers. − Chiral separations can be achieved using chiral mobile phases for the HPLC. , One of the advantages of this method is that the association constant between enantiomers and chiral selectors can be estimated by changing the concentration of chiral selectors in mobile phases. , However, this approach is not mainstream due to the disadvantages of the inconvenience of preparative purpose. Besides HPLC, supercritical fluid chromatography (SFC) has received increasing attention and has played a dominant role in the progress of chromatographic chiral separation due to its high resolution, low organic solvent consumption, short analysis time, and high productivity. − In addition, capillary electrophoresis, capillary electrochromatography, and microchip techniques have also been developed for small-scale separations and used for the microanalysis of chiral compounds. − Furthermore, the recent incremental growth of simulated moving bed (SMB) chromatography has opened up the possibility for industrial-scale chromatographic separations of chiral compounds, which is one of the final goals of the pharmaceutical industry. − …”

High throughput multiplexed method for evaluation of enantioselective performance of chiral selectors by HPLC‐ESI‐MS and dynamic titration: Cinchona alkaloid carbamates discriminating N‐blocked amino acids

“…A sophisticated and widely used technique for this task is high performance liquid chromatography (HPLC). [12][13][14] Analytical HPLC of enantiomeric mixtures can be performed in two different formats: (i) with achiral stationary phase and chiral detector or (ii) with chiral stationary phase (CSP) and achiral detection. A combination of both approaches (i.e., chromatography on CSP with chiral detector) is also possible.…”

Chiral chromatographic separation of β-blockers

“…Nowadays, various CSPs are available and the liquid chromatographic separation of enantiomers on CSPs is known to be the most accurate and convenient means in determining the enantiomeric composition of optically active compounds [26]. Agrawal et al have reviewed broadly various CSPs with respect to their general structure type [27]. Amberlite XAD-4, a nonionic polymeric resin composed of polystyrene chains cross-linked with divinylbenzene has become very popular due to their macroporous structure, excellent size, high rigidity, good hydraulic properties and low cost.…”