Chiral chromatographic separation of β-blockers

Agrawal, Y. K.; Patel, Rinkoo

doi:10.1016/j.jchromb.2005.03.003

Cited by 24 publications

(11 citation statements)

References 30 publications

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“…These compounds are generally commercialized in the racemic form, although one of the enantiomers has an activity 100-fold lower than the other. 41 It is possible that b-blockers will be commercialized in the near future as single enantiomers because of advances in chemical synthesis and potential reductions in side-effects. Therefore, it is important to have methods to determine the enantiomeric purity of such drugs.…”

Section: Indirect Chromatographic Separation After Precolumn Derivatimentioning

confidence: 99%

Fast chiral separation of drugs using columns packed with sub‐2 μm particles and ultra‐high pressure

et al. 2009

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The use of columns packed with sub-2 lm particles in liquid chromatography with very high pressure conditions (known as UHPLC) was investigated for the fast enantioseparation of drugs. Two different procedures were evaluated and compared using amphetamine derivatives and b-blockers as model compounds. In one case, cyclodextrins (CD) were directly added to the mobile phase and chiral separations were carried out in less than 5 min. However, this strategy suffered from several drawbacks linked to column lifetime and low chromatographic efficiencies. In the other case, the analysis of enantiomers was carried out after a derivatization procedure using two different reagents, 2,3,4-tri-O-acetyl-a-D-arabinopyranosyl isothiocyanate (AITC) and N-a-(2,4-dinitro-5-fluorophenyl)-L-alaninamide (Marfey's reagent). Separation of several amphetamine derivatives contained within the same sample was achieved in 2-5 min with high efficiency and selectivity. The proposed approach was also successfully applied to the enantiomeric purity determination of (1)

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Section: Indirect Chromatographic Separation After Precolumn Derivatimentioning

confidence: 99%

Fast chiral separation of drugs using columns packed with sub‐2 μm particles and ultra‐high pressure

et al. 2009

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“…HPLC separations using a chiral stationary phase requires special chiral columns, such as chirobiotic V (vancomycin-based) [10], Chirobiotic T (Teicoplanin-based) [11] and m-[(+)-α-methyl benzyl carboxamide] (XAD-4) [12]. However, chiral columns are expensive and usually high amounts of HPLC grade solvents are required for the elution of the desired compound from such columns.…”

Section: Introductionmentioning

confidence: 99%

“…However, chiral columns are expensive and usually high amounts of HPLC grade solvents are required for the elution of the desired compound from such columns. On the other hand, indirect enantioseparation of ATN in the form of diastereomers has been carried out by RP-HPLC using a variety of chiral derivatizing reagents [12][13][14][15][16][17][18][19]. Derivatiziation reactions, required for such a separation, is time consuming task and the control of the reaction condition such as temperature is urgently necessary to avoid racemization reactions.…”

Section: Introductionmentioning

confidence: 99%

Enantioseparation of atenolol using chiral ligand-exchange chromatography on C8 column

Alizadeh

2013

Separation and Purification Technology

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“…The most recent methods for determination of metoprolol included chromatographic [2][3][4][5] , electrochemical [6,7] and spectrophotometric [8][9][10][11][12][13] techniques. Felodipine is a vasodilator [1] .…”

Section: Introductionmentioning

confidence: 99%

Determination of Metoprolol and Felodipine in Binary Mixture Using Chemometric Assisted Spectrophotometric and High-Performance Liquid Chromatographic-UV Mmethods

Salem¹,

Abdallah²

2007

American J. of Applied Sciences

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Four methods were developed for simultaneous determination of metoprolol and felodipine without previous separation. In the first method both drugs were determined using first derivative UV spectrophotometry, with zero crossing measurement at 222 and 235 nm for metoprolol and felodipine, respectively. The second method depends on first derivative of the ratios spectra by measurements of the amplitudes at 250.1 nm for metoprolol and 224.3 nm for felodipine. Calibration graphs are established in the range of 20-150 µg/ml and 10-60 µg/ml for metoprolol and felodipine, respectively. The third method describes the use of multivariate spectrophotometric calibration for the simultaneous determination of the analyzed binary mixture where the resolution is accomplished by using partial least squares (PLS) regression analysis. Although the components show high degree of spectral overlap, they are simultaneously determined with high accuracy and without interference of pharmaceutical dosage form excipients. A comparison with the related multivariate method of classicial least squares (CLS) analysis is done showing less relative results due to severe spectral overlap of the studied drugs. In the fourth method (HPLC), a reversed-phase column and a mobile phase of methanol:water:acetonitrile (70:22:8 v/v/v/) at 0.9 ml/min flow rate is used to separate both drugs and UV detection at 260 nm. Good linearities are obtained in concentration range of 0.15-15 µg/ml for metoprolol and 0.03-5 µg/ml for felodipine. All the proposed methods are extensively validated. They have the advantage of being economic and time saving. All the described methods can be readily utilized for the analysis of pharmaceutical formulations, the fourth method (HPLC) is successfully applied for the analysis of both drugs in human serum samples. The results obtained by adopting the proposed methods are statistically analyzed and compared with those obtained by reported methods.

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Chiral chromatographic separation of β-blockers

Cited by 24 publications

References 30 publications

Fast chiral separation of drugs using columns packed with sub‐2 μm particles and ultra‐high pressure

Fast chiral separation of drugs using columns packed with sub‐2 μm particles and ultra‐high pressure

Enantioseparation of atenolol using chiral ligand-exchange chromatography on C8 column

Determination of Metoprolol and Felodipine in Binary Mixture Using Chemometric Assisted Spectrophotometric and High-Performance Liquid Chromatographic-UV Mmethods

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