High performance liquid chromatography assay with ultraviolet detection for moxifloxacin: Validation and application to a pharmacokinetic study in Chinese volunteers

Xu, Yu; Liu, Dong; Liu, Xin Yu; Li, Yu Zhen; Lu, Jian

doi:10.1016/j.jchromb.2010.10.024

Cited by 40 publications

(38 citation statements)

References 22 publications

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“…Even though fluorogenic derivatization procedures require heat and longer time, in this study, no heating procedure or long reaction time is needed. Moreover, the RSD, RME, and recovery values indicate that the proposed method is more precise and accurate than some previously reported methods (4)(5)(6)(7)(8)(9). Most of the reported methods also depend on RP-HPLC techniques (3)(4)(5)(6)(7)(8), but differently, the presented method has the shortest retention time.…”

Section: Validation Of the Methodsmentioning

confidence: 62%

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An HPLC method for the determination of moxifloxacin in breast milk by fluorimetric detection with precolumn derivatization

Tekkeli¹,

Gazioğlu²,

Kızıltaş³

2017

Acta Chromatographica

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Summary.A new, sensitive, and selective high-performance liquid chromatography (HPLC) method with fluorimetric detection was developed for the determination of moxifloxacin (MOX) in human breast milk. MOX was precolumn derivatized with fluorescamine; the fluorescent derivative was separated on an RP C18 column using a mobile phase composed of acetonitrile-10 mM orthophosphoric acid by isocratic elution with flow rate of 0.5 mL min −1 . The method was based on the measurement of the derivative using fluorescence detection at 481 nm with excitation at 351 nm. The calibration curve was linear over the range of 1-40 µg mL −1 . Limit of detection (LOD) and limit of quantitation (LOQ) were found to be 0.3 and 1 µg mL −1 , respectively. Intra-day and interday repeatabilities were less than 3.15%.

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Section: Validation Of the Methodsmentioning

confidence: 62%

“…In literature, it is revealed that MOX has been determined in biological fluids: high-performance liquid chromatography (HPLC) [3][4][5][6][7][8][9][10][11], voltammetry [12], and capillary electrophoresis with laser-induced fluorescence [13]. None of them is for breast milk analysis.…”

Section: Introductionmentioning

confidence: 99%

An HPLC method for the determination of moxifloxacin in breast milk by fluorimetric detection with precolumn derivatization

Tekkeli¹,

Gazioğlu²,

Kızıltaş³

2017

Acta Chromatographica

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“…The bactericidal action of Moxi results from trapping of enzymes on DNA and lethal release of doublestranded breaks, thereby inhibiting of cell replication [3]. Various analytical methods have been cited in literature for Moxi determinations including spectrophotometry, spectrofluorimetry, atomic absorption spectrometry, conductometry, voltammetry [4][5][6][7][8][9][10], high performance liquid chromatography-ultraviolet (HPLC-UV), HPLC-fluorescence(HPLC-Fl), capillary electrophoresis(CE), and HPLC-mass spectrometry (HPLC-MS) [4][5][6][7][8][9][10][11][12][13][14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Potentiometric Determination of Moxifloxacin by ZnO Nanorodes Modified ion Selective Electrode

Idress¹,

Elbashir²,

Nur³

2017

Pharm Anal Acta

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Potentiometric determination of moxifloxacin (Moxi) by ion selective electrode based on ZnO nanorods incorporation with HPβ-CD as sensing ionophore and (KTFPB) potassium tetrakis-(3,5(triflouromethyl) phenyl borate ion as anionic site (additive) in polyvinyl chloride (PVC) membrane, without inner reference solution was developed. The sensor shows nearly nernstian response over a concentration range ( 5 × 10 -8 M to 1 × 10 -2 M) with a slope of 21.9 ± 0.16 mv decade -1 of concentration with a limit of detection (LOD) 0.127 μM. The electrode exhibits a fast dynamic response of 2 s for a period of 6 months without significant change in its characteristics with excellent stability and sensitivity toward inorganic species. The method is accurate and precise as indicated by the mean recoveries 99.5% with RSD less than 2%. The proposed method was successfully applied for the determination of Moxi in pure form and its pharmaceutical formulations.

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“…However, most of these methods involve time-consuming sample preparation steps or not usual techniques, e.g., protein precipitation followed by an extra wash step with methylene chloride [6] or prior sample concentration [7], liquid-liquid extraction with 3 mL of ethyl acetate [8] or 5 mL of dichloromethane per sample [9], expensive solid-phase extraction device [5,10,11], and column switching [12]. Use of special additives in mobile phase composition, such as buffers associated to ion pair reagents [11,13], can eventually adsorb to the stationary phase and, thus, reduce column efficiency and lifetime.…”

Section: Introductionmentioning

confidence: 99%

Fast and Sensitive RP-HPLC—Fluorescence Method for the Quantitative Analysis of Moxifloxacin in Rat Plasma and Its Application to a Preclinical Pharmacokinetic Study

Hurtado

Kaiser

Tasso

et al. 2016

Acta Chromatographica

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Summary.A fast, simple, and sensitive reversed-phase high-performance liquid chromatography (RP-HPLC) method has been developed and fully validated for the determination of moxifloxacin (MXF) in rat plasma. MXF and gatifloxacin (internal standard, I.S.) were extracted from plasma by single-step protein precipitation with acidified acetonitrile. Chromatographic separation was accomplished in less than 8 min on an Atlantis ® T3 column with 0.4% aqueous triethylamine-methanol-acetonitrile (60:35:5, v/v/v) solution as mobile phase. Detection was achieved by fluorescence (λ excitation = 295 nm, λ emission = 500 nm), and the calibration curves were found to be linear over the plasma concentration range of 10-2,500 ng mL −1 with a mean correlation coefficient (r) of 0.9946 (n = 6). The intra-and inter-assay imprecision (% CV) was less than 2.4 and 3.3%, respectively, and the accuracy was >90%. The mean extraction recoveries for MXF and I.S. from plasma were 77 and 82%, respectively. The method was also validated for specificity, sensitivity, and stability; all the results were within the acceptable range. The proposed method was then successfully applied to the quantitative analysis of MXF in rat plasma samples, being a valuable and high-throughput assay to support ongoing pharmacokinetic studies on this promising anti-infective agent.

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High performance liquid chromatography assay with ultraviolet detection for moxifloxacin: Validation and application to a pharmacokinetic study in Chinese volunteers

Cited by 40 publications

References 22 publications

An HPLC method for the determination of moxifloxacin in breast milk by fluorimetric detection with precolumn derivatization

An HPLC method for the determination of moxifloxacin in breast milk by fluorimetric detection with precolumn derivatization

Potentiometric Determination of Moxifloxacin by ZnO Nanorodes Modified ion Selective Electrode

Fast and Sensitive RP-HPLC—Fluorescence Method for the Quantitative Analysis of Moxifloxacin in Rat Plasma and Its Application to a Preclinical Pharmacokinetic Study

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