An HPLC method for the determination of moxifloxacin in breast milk by fluorimetric detection with precolumn derivatization

Tekkeli, Şerife Evrim Kepekçi; Gazioğlu, Işıl; Kızıltaş, Mustafa Volkan

doi:10.1556/1326.2017.29.1.4

Cited by 3 publications

(1 citation statement)

References 14 publications

(26 reference statements)

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“…The commissioned procedure in pharmaceutical preparations engages high-performance liquid chromatography [30][31], yet most of the practices published insinuate a niggling mobile phase (buffers) and elaborate detecting modes, besides the inconvenience of the machine for expeditious detection. As a substitute, the electrochemical operations are moderately sensitive, low-priced, convenient, compact, swift response, and consistent for the determination of numerous electroactive medication segments in pharmaceutical dosage patterns and biological fluids.…”

Section: Introductionmentioning

confidence: 99%

Zinc Oxide Nanoparticles Supported on Multi‐walled Carbon Nanotube Modified Electrode for Electrochemical Sensing of a Fluoroquinolone Drug

et al. 2020

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A facile, and novel construction strategy invented on electrochemical procedures for the assemblage of zinc oxide nanoparticles (ZnO NPs) with a multiwalled carbon nanotube modified carbon paste electrode (ZnO-MWCNTMCPE) to ascertain the fluoroquinolone drug-Moxifloxacin (MOX). The ZnO-MWCNTMCPE can significantly improve the electron transfer process and lessen the impedance of the surface of the electrode. Besides, the oxidation of MOX has remarkably enhanced on the modified electrode. Under the optimal experimental conditions, the empirical results exhibited that the oxidation peak current was linearly proportional to the concentration of MOX in the range of 1.0 × 10 À 7 to 2.4 × 10 À 6 mol L À 1 with a detection limit of 0.452 × 10 À 7 mol L À 1. To analyze the practicality of the MOX determination technique in real samples, the intended sensor manipulated for the adjudication of MOX in human urine specimens, and the results authenticated the explore of MOX with the highest accuracy. Further, the proposed sensor system exercised for the simultaneous evaluation of paracetamol (ACOP) and MOX, the DPV technique unveiled the well-separated peaks among ACOP (0.188 mV) and MOX (0.814 mV) with a peak separation (ΔE) of 626 mV in real samples. Therefore, the suggested system could sense and discover the MOX in the biological systems.

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Section: Introductionmentioning

confidence: 99%

Zinc Oxide Nanoparticles Supported on Multi‐walled Carbon Nanotube Modified Electrode for Electrochemical Sensing of a Fluoroquinolone Drug

et al. 2020

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Electrochemical Sensors Based on Molecularly Imprinted Polymers for Pharmaceuticals Analysis

Radi¹,

Wahdan

El-Basiony³

2019

CAC

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Background: The electrochemical sensing of drugs in pharmaceutical formulations and biological matrices using molecular-imprinting polymer (MIP) as a recognition element combined with different electrochemical signal transduction has been widely developed. The MIP electrochemical sensors based on nanomaterials such as graphene, carbon nanotubes, nanoparticles, as well as other electrode modifiers incorporated into the MIPs to enhance the performance of the sensor, have been discussed. The recent advances in enantioselective sensing using MIP-based electrochemical sensors have been described. Methods: The molecular imprinting has more than six decades of history. MIPs were introduced in electrochemistry only in the 1990s by Mosbach and coworkers. This review covers recent literature published a few years ago. The future outlook for sensing, miniaturization and development of portable devices for multi-analyte detection of the target analytes was also given. Results: The growing pharmaceutical interest in molecularly imprinted polymers is probably a direct consequence of its major advantages over other analytical techniques, namely, increased selectivity and sensitivity of the method. Due to the complexity of biological samples and the trace levels of drugs in biological samples, molecularly imprinted polymers have been used to improve the response signal, increase the sensitivity, and decrease the detection limit of the sensors. The emergence of nanomaterials opened a new horizon in designing integrated electrochemical systems. The success of obtaining a high-performance electrochemical sensor based on MIPs lies in the kind of material that builds up the detection platform. Conclusion: The novel approaches to produce MIP materials, combined with electrochemical transduction to develop sensors for screening different pharmaceutically active compounds have been overviewed. MIPs may appear indispensable for sensing in harsh conditions, or sensing that requires longterm stability unachievable by biological receptors. The electrochemical sensors provide several benefits including low costs, shortening analysis time, simple design; portability; miniaturization, easy-touse, can be tailored using a simple procedure for particular applications. The performance of sensor can be improved by incorporating some conductive nanomaterials as AuNPs, CNTs, graphene, nanowires and magnetic nanoparticles in the polymeric matrix of MIP-based sensors. The application of new electrochemical sensing scaffolds based on novel multifunctional-MIPs is expected to be widely developed and used in the future.

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Determination of Levofloxacin by HPLC with Fluorescence Detection in Human Breast Milk

2021

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Aim: A new HPLC method with fluorescence detection has been developed and validated for the determination of levofloxacin, one of the fluoroquinolone class antibiotics, in breast milk. Materials & methods: Chromatographic separation was carried out on a reversed phase C18 column with acetonitrile and 10 mM o-phosphoric acid (25:75,v/v) mobile phase composition. Moxifloxacin was used as internal standard and the peaks were detected by fluorescence detection. Results & conclusion: Calibration graph was found linearly within the range of 2.5–500 ng/ml. Limit of detection and limit of quantification were found to be 0.63 and 2.11 ng/ml, respectively. Mean absolute recovery was 96.18%. The developed method has been successfully applied to the determination of levofloxacin in human breast milk taken from two healthy volunteers.

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An HPLC method for the determination of moxifloxacin in breast milk by fluorimetric detection with precolumn derivatization

Cited by 3 publications

References 14 publications

Zinc Oxide Nanoparticles Supported on Multi‐walled Carbon Nanotube Modified Electrode for Electrochemical Sensing of a Fluoroquinolone Drug

Zinc Oxide Nanoparticles Supported on Multi‐walled Carbon Nanotube Modified Electrode for Electrochemical Sensing of a Fluoroquinolone Drug

Electrochemical Sensors Based on Molecularly Imprinted Polymers for Pharmaceuticals Analysis

Determination of Levofloxacin by HPLC with Fluorescence Detection in Human Breast Milk

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