High-performance liquid chromatographic separation of rhubarb constituents

Ohshima, Yukio; Ohno, Yohei; Kajiyama, Kiichiro; Takahashi, Kunio

doi:10.1016/s0021-9673(00)91680-7

Cited by 24 publications

(8 citation statements)

References 2 publications

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“…1,2) Some naturally-occurring anthraquinones have already been examined by thin layer chromatography, 3) HPLC methods, [4][5][6][7][8] and capillary electrophoresis (CE), [9][10][11][12][13][14] however, only a single component or limited components have been determined in the majority of them. A few simultaneous analyses of different types of components have been conducted.…”

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“…A few simultaneous analyses of different types of components have been conducted. 6,8,14) Studies related to simultaneous determination by CE are especially rare. Rhei Rhizoma contains a wide variety of components, including monomeric anthraquinones, their glycosides, and dimeric anthrone glycosides, and thus simultaneous determination of these different groups of components showing large differences in polarity is desirable for the quality control of the herb.…”

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Simultaneous Determination of Anthraquinones, Their 8-.BETA.-D-Glucosides, and Sennosides of Rhei Rhizoma by Capillary Electrophoresis

Koyama

Morita

Fujiyoshi

et al. 2005

CHEMICAL & PHARMACEUTICAL BULLETIN

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In a continuation of our work on quinones that occur widely in the plant kingdom and that may have biological activities, we have developed a simple cyclodextrin modified capillary zone electrophoresis (CD-CZE) to separate and determine emodin, chrysophanol, aloe-emodin, rhein, emodin-8-b-D-glucoside, chrysophanol-8-b-D-glucoside, rhein-8-b-Dglucoside, sennoside A, and sennoside B of Rhei Rhizoma. Rhei Rhizoma is an old-and well-known herbal medicine known for its purgative effect due to anthracene derivatives, especially their glycosides. The analysis of these compounds and anthraquinones in pharmaceutical preparations or in complex matrices such as crude plant extracts is, therefore, of special interest.1,2) Some naturally-occurring anthraquinones have already been examined by thin layer chromatography, 3) HPLC methods, [4][5][6][7][8] and capillary electrophoresis (CE), [9][10][11][12][13][14] however, only a single component or limited components have been determined in the majority of them. A few simultaneous analyses of different types of components have been conducted. 6,8,14) Studies related to simultaneous determination by CE are especially rare. Rhei Rhizoma contains a wide variety of components, including monomeric anthraquinones, their glycosides, and dimeric anthrone glycosides, and thus simultaneous determination of these different groups of components showing large differences in polarity is desirable for the quality control of the herb. For this purpose, we have developed a simple and rapid CD-CZE method (except for rhein) using 0.005 M a-CD in 0.03 M borate buffer (pH 10.0) containing 20% acetonitrile. ExperimentalReagents and Materials Sodium tetraborate, sodium dodecyl sulphate (SDS), sodium hydroxide, hydroxypropyl-b-CD (HP-b-CD), g-CD, and HPg-CD were purchased from Wako Pure Chemicals (Osaka, Japan), a-CD, b-CD, and sennoside A (8) from Nacalai Tesque (Kyoto, Japan), sennoside B (9) from Funakoshi (Tokyo, Japan), and boric acid from Fluka (Buchs, Switzerland). Acetonitrile, methanol, and water were of HPLC grade. Emodin (1) and chrysophanol (2) were isolated from the root bark of Cassia siamea.15) Emodin-8-b-D-glucoside (5) was isolated from the root of Polygonum cuspidatum SIEB. et ZUCC. 16,17) and chrysophanol-8-b-D-glucoside (6) from Cascara Sagrada (the root of Rhamnus purshiana DC.).18,19) Aloeemodin (3), rhein (4), and rhein-8-b-D-glucoside (7) were isolated from Rhei Rhizoma 20) (Fig. 1). Rhei Rhizoma (Rheum palmatum L., Rheum tanguticum MAXIM.) was purchased from The Iguchi Pharmacy (Kobe, Japan).Procedure for CE The CE analyses were carried out using a Beckman MDQ and P/ACE System 5000 apparatus (Fullerton, CA, U.S.A.) equipped with a UV detector set at 254 nm, a diode array detector, and a Beckman untreated fused-silica capillary (570 mmϫ75 mm i.d.; 500 mm effective length). The analytical conditions were as follows: sampling time, 5 s (hydrodynamic mode; 0.5 p.s.i.); applied constant voltage, 20 kV; column temperature, 20°C. The CD-CZE electrolyte was a buffer solution prepared b...

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Simultaneous Determination of Anthraquinones, Their 8-.BETA.-D-Glucosides, and Sennosides of Rhei Rhizoma by Capillary Electrophoresis

Koyama

Morita

Fujiyoshi

et al. 2005

CHEMICAL & PHARMACEUTICAL BULLETIN

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“…10 High-performance liquid chromatography (HPLC) for determination of five free anthraquinones is frequently used for quality control of Radix et Rhizoma Rhei according to the Chinese Pharmacopoeia and related literatures. 1,9,[11][12][13][14][15][16][17][18][19] Moreover, thin-layer chromatography (TLC), 20,21 capillary electrophoresis (CE), 22,23 ultra-performance liquid chromatography (UPLC), 6,24 UPLC-mass spectrometry (MS), 3 and high speed counter current chromatography (HSCCC) 25 also have been reported for determination of the main bioactive components in Radix et Rhizoma Rhei. However, all the analytical methods above for determining and quantifying Radix et Rhizoma Rhei suffer from the intrinsic limitations of chromatography-based methods, namely, the need of standard compounds for analyte identification or quantification and the relatively long analysis times.…”

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Simultaneous, Simple and Rapid Determination of Five Bioactive Free Anthraquinones in Radix et Rhizoma Rhei by Quantitative¹H NMR

Dong¹,

Cai²,

Fang³

et al. 2016

Journal of the Brazilian Chemical Society

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Radix et Rhizoma Rhei has been recognized for centuries in traditional medicine for its multiple pharmacological actions. The free anthraquinones including physcion, chrysophanol, emodin, rhein, and aloe-emodin are the main bioactive components in Radix et Rhizoma Rhei. In the present study, a fast quantitative 1 H nuclear magnetic resonance (q-HNMR) method for the determination and quantitation of five free anthraquinones in Radix et Rhizoma Rhei was developed. Validation of the quantitative method was performed in terms of specificity, accuracy, precision, and stability. The results showed that the solvent acetone-d 6 enabled satisfactory separation of the signals to be integrated. Five anthraquinones in Radix et Rhizoma Rhei could be quantified accurately using featured signals from 1 H NMR. This work implied that q-HNMR represents a feasible alternative to high-performance liquid chromatography (HPLC)-based methods for quantitation of anthraquinones in Radix et Rhizoma Rhei and is suitable for the quality control of Radix et Rhizoma Rhei.

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“…The pharmaceutically bioactive components in Rhubarb are hydroxyanthraquinone derivatives: emodin, chrysophanol, physcion, rhein, aloe-emodin and their glycosides. TLC [2] and HPLC [3,4] have been commonly used for the separation and determination of active components in Rhubarb.…”

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confidence: 99%

Determination of Active Components in Rhubarb by Cyclodextrin-modified Capillary Zone Electrophoresis

Shang

Yuan

2001

Sensors

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A cyclodextrin-modified capillary zone electrophoresis was developed for the determination of four bioactive components in Rhubarb. Effects of pH, β-CD concentration and organic modifier content on the migration and separation of the components were studied. The four components were separated in the running buffer of 50 mmol/L NaOH-H 3 BO 3 (pH 10.7) containing 10 mmol/L β-CD and 12.5% v/v ethanol. The analytical performance of the method was tested with respect to linearity response, precision and recovery. The determination of two commercial Rhubarb samples under the optimized conditions showed a satisfactory result.

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High-performance liquid chromatographic separation of rhubarb constituents

Cited by 24 publications

References 2 publications

Simultaneous Determination of Anthraquinones, Their 8-.BETA.-D-Glucosides, and Sennosides of Rhei Rhizoma by Capillary Electrophoresis

Simultaneous Determination of Anthraquinones, Their 8-.BETA.-D-Glucosides, and Sennosides of Rhei Rhizoma by Capillary Electrophoresis

Simultaneous, Simple and Rapid Determination of Five Bioactive Free Anthraquinones in Radix et Rhizoma Rhei by Quantitative¹H NMR

Determination of Active Components in Rhubarb by Cyclodextrin-modified Capillary Zone Electrophoresis

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High-performance liquid chromatographic separation of rhubarb constituents

Cited by 24 publications

References 2 publications

Simultaneous Determination of Anthraquinones, Their 8-.BETA.-D-Glucosides, and Sennosides of Rhei Rhizoma by Capillary Electrophoresis

Simultaneous Determination of Anthraquinones, Their 8-.BETA.-D-Glucosides, and Sennosides of Rhei Rhizoma by Capillary Electrophoresis

Simultaneous, Simple and Rapid Determination of Five Bioactive Free Anthraquinones in Radix et Rhizoma Rhei by Quantitative1H NMR

Determination of Active Components in Rhubarb by Cyclodextrin-modified Capillary Zone Electrophoresis

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Simultaneous, Simple and Rapid Determination of Five Bioactive Free Anthraquinones in Radix et Rhizoma Rhei by Quantitative¹H NMR