High-performance liquid chromatographic separation and electrochemical detection of penicillins

Kirchmann, Eric; Welch, Lawrence E.

doi:10.1016/0021-9673(93)83144-h

Cited by 23 publications

(5 citation statements)

References 34 publications

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“…Pulsed amperometric detection with the Ag/AgCl reference electrode was applied for the determination of AMO, AMP, METH, PEN-G, PEN-V, OXA, CLO, NAF and DICLO in drug standards [53].…”

Section: Detection Techniquesmentioning

confidence: 99%

Chromatographic analysis of penicillins in pharmaceutical formulations and biological fluids

Samanidou

Evaggelopoulou

Papadoyannis³

2006

J of Separation Science

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Natural penicillin (benzylpenicillin) is the oldest antibiotic observed by Alexander Fleming in 1928. To broaden its spectrum of activity, natural penicillin was modified, giving rise to a group of antibiotics under the name 'penicillins'. Although an increasing number of bacteria appear to be resistant to them, penicillins are used to treat a variety of bacterial infections including Gram-positive, Gram-negative aerobic and anaerobic bacteria. Consequently, they are widely used in human and veterinary medicine to prevent and treat diseases. This review covers the analytical methodologies, mainly chromatographic, employed to the penicillins determination in pharmaceutical formulations, biological fluids and in production-scale fermentations reported in the literature. Results of published assays are comparatively presented focusing on sample preparation regarding isolation and purification, chromatographic conditions and method validation. Information on chemical structure, spectrum of activity and action mechanism of common penicillins has also been given.

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“…Pulsed amperometric detection with the Ag/AgCl reference electrode was applied for the determination of AMO, AMP, METH, PEN-G, PEN-V, OXA, CLO, NAF and DICLO in drug standards [53].…”

Section: Detection Techniquesmentioning

confidence: 99%

Chromatographic analysis of penicillins in pharmaceutical formulations and biological fluids

Samanidou

Evaggelopoulou

Papadoyannis³

2006

J of Separation Science

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“…Current extraction techniques for antibiotics from milk can be difficult to perform, require the use of toxic solvents, or not be adequately sensitive for the determination. Reports of extractions of cephapirin from milk and β-lactam antibiotics from milk − and tissues , have been published. Many of these protocols rely on low-wavelength UV detection for the liquid chromatographic analysis step and therefore require difficult sample preparation protocols ,,− or postcolumn derivatization , in order to obtain adequate sensitivity to accomplish the analysis.…”

mentioning

confidence: 99%

Assay for Cephapirin and Ampicillin in Raw Milk by High-Performance Liquid Chromatography−Integrated Pulsed Amperometric Detection

Dasenbrock¹,

LaCourse²

1998

Anal. Chem.

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The FDA has issued guidelines governing the use of antibiotics in cattle and routinely tests for the presence of antibiotics in milk. Unfortunately, these compounds are often difficult to detect by direct methods because they often lack a chromophore or fluorophore. Integrated pulsed amperometric detection (IPAD) following reversed-phase liquid chromatography is well-suited for this analysis because it is selective, sensitive, and direct; i.e., derivatization is not required. This work involves the development of a simple, rapid assay for the determination of beta-lactam antibiotic residues in milk using HPLC-IPAD, specifically, ampicillin and cephapirin. Since the analyst studied here are detectable by UV detection, a comparison between IPAD and UV detection will be made. Sample preparation schemes that involve the extraction of antibiotics of interest from the milk matrix and subsequent cleanup are an important aspect of this project. These procedures will be discussed in detail. In addition, analytical figures of merit and IPAD wave form optimization will be addressed.

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“…It is also used in combination with other medications to eliminate H. pylori, a bacteria that causes ulcers. A group of penicillins covering amoxicillin and ampicillin are used for fighting bacterial infections in various domestic animals; 1 this can cause the transfer of penicillins to milk and meat products by and from the animals. Therefore, the development of a simple and sensitive method is of great importance for trace determination of Amoxicillin in low dose pharmaceutical formulation and in therapeutic drug monitoring in routine clinical practice.…”

mentioning

confidence: 99%

Application of Montmorillonite Clay and Mesoporous Carbon as Modifiers to Carbon Paste Electrode for Determination of Amoxicillin Drug

Abdel-Galeil

El‐Desoky

Ghoneim

et al. 2017

J. Electrochem. Soc.

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Two types of mesoporous carbon (MC and MC − ) were prepared. MC was synthesized using the prepared SBA-15 as a hard template and sucrose as the carbon source. While, MC − has been fabricated using polyethylene-polypropylene glycol (F127) as a soft template and resorcinol as a carbon source. The prepared mesoporous carbon and commercial sodium montmorillonite clay (Na-MMT) were characterized using X-ray diffraction (XRD), N 2 adsorption isotherm (BET), scanning electron microscope (SEM) and transmission electron microscope (TEM). Carbon paste electrode modified with MC, MC − as well as Na-MMT were used as sensors for determination of amoxicillin drug using linear sweep and square wave adsorptive anodic stripping voltammetry (LS-and SW-AdASV) methods. The most developed peak current was observed at MC modified carbon paste electrode (MC/CPE) indicating its strongest adsorptive property which can be attributed to its highest surface area and lowest charge transfer resistance. MC/CPE was successfully applied for the determination of amoxicillin drug in its pharmaceutical formulation and in biological fluids. LOD values of 6.0 × 10 −9 and 1.5 × 10 −9 M bulk amoxicillin were obtained using LS-and SW-AdASV, respectively.

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High-performance liquid chromatographic separation and electrochemical detection of penicillins

Cited by 23 publications

References 34 publications

Chromatographic analysis of penicillins in pharmaceutical formulations and biological fluids

Chromatographic analysis of penicillins in pharmaceutical formulations and biological fluids

Assay for Cephapirin and Ampicillin in Raw Milk by High-Performance Liquid Chromatography−Integrated Pulsed Amperometric Detection

Application of Montmorillonite Clay and Mesoporous Carbon as Modifiers to Carbon Paste Electrode for Determination of Amoxicillin Drug

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