High-performance liquid chromatographic determination of amantadine in urine after micelle-mediated pre-column derivatization with 1-fluoro-2,4-dinitrobenzene

Horst, F.A.L. van der; Teeuwsen, J.; Holthuis, J.J.M.; Brinkman, U.A.Th.

doi:10.1016/0731-7085(90)80124-8

Cited by 36 publications

(14 citation statements)

References 14 publications

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“…Most HPLC methods involved dansyl chloride [4], o-phthalaldehyde (OPA) [10,11], 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) [12] and 1-fluoro-2,4-dinitrobenzene (DNFB) [21] as derivatization reagents to react with tricyclic amines. In this study, dansyl chloride, 4-fluoro-7-nitro-2,1,3-benzoxadiazole and 1-fluoro-2,4-dinitrobenzene were tested to react with memantine hydrochloride and we found that FMOC-Cl derivates of memantine hydrochloride had advantages of higher sensitivity, less interference, and simpler reaction conditions than others.…”

Section: Discussionmentioning

confidence: 99%

High‐performance liquid chromatographic determination of memantine hydrochloride in rat plasma using sensitive fluorometric derivatization

Xie

Zhou

Tong

et al. 2011

J of Separation Science

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In this study, we investigated a simple, sensitive and reliable liquid chromatography-fluorescence detection method for the determination of memantine hydrochloride in rat plasma which was based on derivatization with 9-fluorenylmethyl chloroformate (FMOC-Cl). For the first time, FMOC-Cl was introduced into derivatization of memantine hydrochloride in rat plasma. The amino groups of memantine hydrochloride and amantadine hydrochloride (internal standard) were trapped with FMOC-Cl to form memantine hydrochloride-FMOC-Cl and amantadine hydrochloride-FMOC-Cl compositions, which can be very compatible for LC-FLD. Precipitation of plasma proteins by acetonitrile was followed by vortex mixing and centrifugation. Chromatographic separation was performed on a C(18) column (DIAMONSIL 150 × 4.6 mm, id 5 μm) with a mobile phase consisting of acetonitrile and water at a flow rate of 1.0 mL/min. The retention times of memantine hydrochloride-FMOC-Cl and amantadine hydrochloride-FMOC-Cl compositions were 23.69 and 40.27 min, respectively. Optimal conditions for the derivatization of memantine hydrochloride were also described. The limit of quantification (LOQ) was 25 ng/mL for memantine hydrochloride in plasma, the linear range was 0.025-5.0 μg/mL in plasma with a correlation coefficient (r) of 0.9999. The relative standard deviations (RSDs) of intra-day and inter-day assays were 4.46-12.19 and 5.23-11.50%, respectively. The validated method was successfully applied to the determination of memantine hydrochloride in rat plasma samples.

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Section: Discussionmentioning

confidence: 99%

High‐performance liquid chromatographic determination of memantine hydrochloride in rat plasma using sensitive fluorometric derivatization

Xie

Zhou

Tong

et al. 2011

J of Separation Science

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“…Therefore, the use of a derivatization reagent which is reactive toward amino group and the more popularized system would seem suitable for determination of 1-ADA concentration in biological fluids. Van Der Horst et al have shown the determination of 1-ADA in urine using high-performance liquid chromatography (HPLC) by derivatization with 1-fluoro-2,4-dinitrobenzene (DNFB) as an UV-labeled agent [14]. But, the utility of DNFB resulted in producing an amount of precipitation during the derivatization, and interference peaks were detected on chromatograms.…”

Section: Introductionmentioning

confidence: 98%

Liquid chromatographic determination of 1-adamantanamine and 2-adamantanamine in human plasma after pre-column derivatization with o-phthalaldehyde and 1-thio-β-d-glucose

Higashi

Fujii

2004

Journal of Chromatography B

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“…Therefore, derivation of AMA is commonly adopted before it is analyzed by HPLC, gas chromatography, capillary electrophoresis, potentiometry, and fluorometry. Several derivatizing reagents have been intensively studied for HPLC analysis of AMA, including o-phthalaldehyde and 1-thio-β-D-glucose, 11 (2-naphthoxy) acetyl chloride, 12 anthraquinone-2-sulfonyl chloride, 13 dansyl chloride, 14 1-fluoro-2,4-dinitrobenzene, 15 and 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F). 16 Among those, NBD-F selectively reacts with the primary and secondary amino groups and has been successfully used for the analyses of many compounds.…”

Section: Introductionmentioning

confidence: 99%

Determination of Amantadine Residue in Honey by Solid‐phase Extraction and High‐performance Liquid Chromatography with Pre‐column Derivatization and Fluorometric Detection

et al. 2011

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Amantadine (AMA) is an anti-viral drug used in apiculture to protect honeybee against the sacbrood virus (Morator aetatulae). This study described a reliable high-performance liquid chromatographic (HPLC) method for analyzing AMA in honey using a solid-phase extraction (SPE) cartridge (Plexa PCX) for purification, 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) as a pre-column derivatization agent, and fluorometric detection (λ ex ＝470 nm, λ em ＝530 nm). The chromatographic separation was performed on an XDB C18 column (150×4.6 mm i.d.) using 0.1% trifluoroacetic acid/acetonitrile (35∶65, V/V) as the mobile phase at a flow rate of 1.0 mL•min -1 with a run time of 20 min. Under these optimal conditions, a linear relationship was observed in the range of 0.025-1.0 µg• mL -1 with a good correlation coefficient (0.998) and low limit of detection (0.0080 µg•g -1 ), the recoveries were all above 90%, and the intra-day and inter-day precision (RSD) ranged from 3.4%-5.1%.

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High-performance liquid chromatographic determination of amantadine in urine after micelle-mediated pre-column derivatization with 1-fluoro-2,4-dinitrobenzene

Cited by 36 publications

References 14 publications

High‐performance liquid chromatographic determination of memantine hydrochloride in rat plasma using sensitive fluorometric derivatization

High‐performance liquid chromatographic determination of memantine hydrochloride in rat plasma using sensitive fluorometric derivatization

Liquid chromatographic determination of 1-adamantanamine and 2-adamantanamine in human plasma after pre-column derivatization with o-phthalaldehyde and 1-thio-β-d-glucose

Determination of Amantadine Residue in Honey by Solid‐phase Extraction and High‐performance Liquid Chromatography with Pre‐column Derivatization and Fluorometric Detection

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