High‐performance liquid chromatographic determination of memantine hydrochloride in rat plasma using sensitive fluorometric derivatization

Abstract

In this study, we investigated a simple, sensitive and reliable liquid chromatography-fluorescence detection method for the determination of memantine hydrochloride in rat plasma which was based on derivatization with 9-fluorenylmethyl chloroformate (FMOC-Cl). For the first time, FMOC-Cl was introduced into derivatization of memantine hydrochloride in rat plasma. The amino groups of memantine hydrochloride and amantadine hydrochloride (internal standard) were trapped with FMOC-Cl to form memantine hydrochloride-FMOC-Cl and amantadine hydrochloride-FMOC-Cl compositions, which can be very compatible for LC-FLD. Precipitation of plasma proteins by acetonitrile was followed by vortex mixing and centrifugation. Chromatographic separation was performed on a C(18) column (DIAMONSIL 150 × 4.6 mm, id 5 μm) with a mobile phase consisting of acetonitrile and water at a flow rate of 1.0 mL/min. The retention times of memantine hydrochloride-FMOC-Cl and amantadine hydrochloride-FMOC-Cl compositions were 23.69 and 40.27 min, respectively. Optimal conditions for the derivatization of memantine hydrochloride were also described. The limit of quantification (LOQ) was 25 ng/mL for memantine hydrochloride in plasma, the linear range was 0.025-5.0 μg/mL in plasma with a correlation coefficient (r) of 0.9999. The relative standard deviations (RSDs) of intra-day and inter-day assays were 4.46-12.19 and 5.23-11.50%, respectively. The validated method was successfully applied to the determination of memantine hydrochloride in rat plasma samples.

Brompheniramine as a novel probe for indirect UV detection and its application for the capillary electrophoresis of adamantane drugs

“…Analytical methods for assays of memantine, amantadine, and rimantadine are available in pharmacopeias (e.g. The United States Pharmacopeia (USP) and British Pharmacopoeias (BP) ) and the literature . Most methods avoid the use of optical detection (e.g.…”

Development of an LC–MS/MS method for simultaneous determination of memantine and donepezil in rat plasma and its application to pharmacokinetic study

“…To date, numerous reports have been published to determine the concentration of MM and DPZ individually in several biomatrices by using instruments such as high performance liquid chromatography (HPLC) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) employing various techniques such as fluorimetric derivatization, solid-phase extraction, automated liquidliquid extraction (LLE) and so on [11][12][13][14][15][16][17][18][19][20]. However, to the best of our knowledge, reports determining their simultaneous estimation have not been published yet.…”

Spectrophotometric Determination of Alzheimer’s Drug, Memantine Hydrochloride in Biological Samples Using Ninhydrin and Ferric Chloride

“…[6] High performance liquid chromatographic methods coupled with fluorescence detection have been reported in the literature for the quantification of MEM in rat plasma, [7,8] human plasma. [9][10][11] Determination of memantine in human plasma by liquid chromatography with mass spectrometry (LC-MS), [12][13][14][15] gas chromatography with mass spectrometry (GC-MS) [16] and micellar electro kinetic chromatography (MEKC) [17], extractive-Spectrophotometric [18] oxidation-reduction reaction-spectrophotometric [19] or Potentiometrically [20,21].…”