High-performance liquid chromatographic determination of histamine in biological samples: The cerebrospinal fluid challenge – A review

Tables, and 27 References A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPTABSTRACT Background: Histamine (HA) is a small amine playing an important role in anaphylactic reactions. In order to identify and quantify HA in plasma matrix, different methods have been developed but present several disadvantages. Here, we developed an alternative method using liquid chromatography coupled with an ultra-high resolution and accurate mass instrument, Q Exactive TM (Thermofisher) (LCHRMS). Methods:The method includes a protein precipitation of plasma samples spiked with HA-d4as internal standard (IS). LC separation was performed on a C18 Accucore column (100*2.1 mm, 2.6 μm) using a mobile phase containing nonafluoropentanoic acid (3 nM) and acetonitrile with 0.1% (v/v) formic acid on gradient mode. Separation of analytes was obtained within 10 min. Analysis was performed from full scan mode and targeted MS2 mode using a 5 ppm mass window. Ion transition monitored for targeted MS2 mode were 112.0869>95.0607m/z for HA and 116.1120>99.0855m/z for HAd4. Calibration curves were obtained by adding standard calibration dilution at 1 to 180 nM in TrisBSA.Results: Elution of HA and IS occurred at 4.1 min. The method was validated over a range of concentrations from 1 nM to 100 nM. The intra-and inter-run precisions were <15% for quality controls. Human plasma samples from 30 patients were analysed by LCHRMS, and the results were highly correlated with those obtained using the gold standard Radioimmunoassay (RIA) method. Conclusion:Overall, we demonstrate here that LCHRMS is a sensitive method for histamine quantification in biological human plasmas, suitable for routinely use in medical laboratories. In addition, LCHRMS is less time-consuming than RIA, avoid the use of radioactivity, and could then be considered as an alternative quantitative method.

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“…However, most of HA quantification methods used for medical applications needs a derivatization step [7] …”

Section: Accepted M Manuscriptmentioning

confidence: 99%

“…HA release could be measured in plasma, but also in other matrix such as urine [6], cerebrospinal fluid [7], or even cell culture supernatant [8]. Due to HA low concentrations in these different fluids even after an important release, sensitive quantification methods are needed.…”

Section: Introductionmentioning

confidence: 99%

Histamine quantification in human plasma using high resolution accurate mass LC–MS technology

Laurichesse

Gicquel

Moreau

et al. 2016

Clinical Biochemistry

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“…Based on the assessment of poisoning cases, the guidance levels suggested for histamine content in seafood are: <50 ppm for safe consumption; 200-1000 ppm as probably toxic; and >1000 ppm as toxic and unsafe for human consumption [8,9]. Several analytical methods have been developed for the analysis and determination of histamine levels such as ultrasensitive flow injection electrochemical [10], lignin modified glassy carbon electrode [11], thin layer chromatography (TLC) [12], high performance liquid chromatography (HPLC) [13], 1 H NMR [14], electrochemiluminescence [15], liquid chromatography tandem mass spectrometry [16], polymerase chain reaction/denaturing gradient gel electrophoresis (PCR-DGGE) [3] and spectrophotometry [17]. Complex reaction between histamine, Co(II) and oxalate has been reported [18] with the complex structure shown (Figure 1), but it has not given a significant change of wavelength and solution color.…”

Section: Introductionmentioning

confidence: 99%

Rapid Spectrophotometric Method for Histamine Determination in Fish Using Alizarin Red S and Metal

2019

MJAS

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An analytical method for the determination of histamine using metals and alizarin red S (ARS) reagents by UV-Visible spectrophotometry was developed. Cu(II), Co(II) and ARS were used to form colored complex with histamine. The developed method was used to detect qualitatively and quantitatively the presence of histamine. Absorbance of Cu(II) and Co(II) complex were measured at a maximum wavelength of 505.5 and 567 nm, respectively. Optimization of analytical parameters such as concentration of metals, concentration of ARS, sample solution pH, and optimum time for complex formation were performed. Experimental results showed that the optimum concentration of Cu(II) was 50 ppm, 75 ppm ARS, sample solution pH 6, and 15 minutes optimum time, while the optimum concentration of Co(II) was 125 ppm, ARS was 75 and 50 ppm, sample solution pH 5 and 10 minutes optimum time. Method validation indicated that the coefficient of variation, limit of detection (LOD) (S/N =3), and limit of quantitation (LOQ = 10S/N) of Cu(II) were 0.65%, 8.94 ppm, and 29.82 ppm respectively with a sensitivity of 0.0054 ppm and linearity of 0.9959. Accuracy of histamine determination through Cu-ARS-histamine complex at a concentration of 50, 100, and 150 ppm were 105.7% (RSD = 0.777%, n = 3), 105.06% (RSD = 0.606%, n = 3), and 94.12% (RSD = 0.767%, n = 3), respectively. Meanwhile, the CV, LOD, and LOQ of Co(II) were 0.28%; 2.58 ppm; and 8.6 ppm respectively with a sensitivity of 0.0006 ppm and linearity of 0.9965. Accuracy of histamine determination through Co-ARS-histamine complex at a concentration of 50, 75, and 125 ppm were 98.33%, 95.83%, 93.88%, respectively. The proposed method was successfully applied to the determination of histamine in a fish sample with quantitative recovery; for Cu(II) complex (99.23%) and Co(II) complex (102.62%).

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“…3,[7][8][9][10][11][12][13] However, differ somatic cell, differ the value of histamine. As we all known, histamine almost existed in mast cells and basophils, the content of that in other somatic cell was low relatively.…”

Section: Introductionmentioning

confidence: 99%

Development of Highly Sensitive Analysis Method for Histamine and Metabolites in Pregnant Women’s Fingernail by UPLC-ESI-MS

Uchibe

Shi

et al. 2018

ANAL. SCI.

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In this study, a highly sensitive analysis method for the rapid detection of histamine (HA), imidazole-4-acetic acid (IAA) and 1-methylhistamine (MHA) in pregnant women fingernails was developed by the ultra-performance liquid chromatography ( coefficient were found to be above 0.9998, the measured detection limit for derivatized histamine and metabolites ranged from 0.06 fmol to 0.15 fmol, and the relative standard derivations of intra-day and inter-day assay were 6.3%. Furthermore, the mean recoveries (%) of the standards added to human fingernails were in the range of 90.2-100.5%. The validatedmethod was successfully applied to analyze human fingernail samples from 3 pregnant women and 3 healthy women. The report about the detection of histamine and metabolites in the fingernails in pregnant women fingernails was the first published.

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High-performance liquid chromatographic determination of histamine in biological samples: The cerebrospinal fluid challenge – A review

Cited by 21 publications

References 42 publications

Histamine quantification in human plasma using high resolution accurate mass LC–MS technology

Histamine quantification in human plasma using high resolution accurate mass LC–MS technology

Rapid Spectrophotometric Method for Histamine Determination in Fish Using Alizarin Red S and Metal

Development of Highly Sensitive Analysis Method for Histamine and Metabolites in Pregnant Women’s Fingernail by UPLC-ESI-MS

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