Histamine quantification in human plasma using high resolution accurate mass LC–MS technology

Laurichesse, Mathieu; Gicquel, Thomas; Moreau, Caroline; Tribut, Olivier; Tarte, Karin; Morel, Isabelle; Bendavid, Claude; Amé-Thomas, Patricia

doi:10.1016/j.clinbiochem.2015.08.012

Cited by 7 publications

(7 citation statements)

References 26 publications

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“…In conclusion, besides a slight underestimation of HA concentrations measured by LC-HRMS, LC-HRMS gave particularly encouraging results, as a good correlation with RIA was found [18].…”

Section: Comparison Of Lc-hrms To Riamentioning

confidence: 70%

“…Finally, some methods using mass spectrometry have been developed to quantify plasma HA [16,17]. In particular, our team developed an in-house plasma HA quantification assay for diagnostic use based on high Resolution Accurate Mass LC-MS (LC-HRMS) technology [18]. The LC-HRMS method is known to be highly sensitive and specific, averts radioactivity use, and avoids the potential detection of the metabolites of the targeted protein.…”

Section: Graphical Abstractmentioning

confidence: 99%

“…According to the manufacturers' data, cross-reactivity with the metabolite methyl histamine is estimated at 0.1% in Demeditec Diagnostics ® EIA, and cross-reactivity to acylated methyl histamine is estimated at 0.027%, and 0.069% in Immunotech ® EIA and RIA, respectively. LC-HRMS is the only assay that can distinguish methyl histamine from HA [16][17][18]. This could explain the trend of LC-HRMS to slightly underestimate plasma HA concentrations in comparison to RIA.…”

Section: Comparison Of the 3 Tested Assays To Riamentioning

confidence: 99%

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Comparison of two enzymatic immunoassays, high resolution mass spectrometry method and radioimmunoassay for the quantification of human plasma histamine

Poli

Laurichesse

Rostan

et al. 2016

Journal of Pharmaceutical and Biomedical Analysis

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Histamine (HA) is one of the main immediate mediators involved in allergic reactions. HA plasma concentration is well correlated with the severity of vascular and respiratory signs of anaphylaxis. Consequently, plasma quantification of HA is useful to comfort the diagnosis of anaphylaxis. Currently, radioimmunoassay (RIA) is the gold standard method to quantify HA due to its high sensitivity, but it is time consuming, implicates specific formations and cautions for technicians, and produces hazardous radioactive wastes. The aim of this study was to compare two enzymatic immunoassays (EIA) and one in-house liquid chromatography high-resolution mass spectrometry method (LC-HRMS) with the gold standard method for HA quantification in plasma samples of patients suspected of anaphylaxis reactions. Ninety-two plasma samples were tested with the 4 methods (RIA, 2 EIA and LC-HRMS) for HA quantification. Fifty-eight samples displayed HA concentrations above the positive cut-off of 10nM evaluated by RIA, including 18 highly positive samples (>100 nM). This study shows that Immunotech(®) EIA and LC-HRMS concentrations were highly correlated with RIA values, in particular for samples with a HA concentration around the positive cut-off. In our hands, plasma concentrations obtained with the Demeditec Diagnostics(®) EIA correlated less with results obtained by RIA, and an underestimation of plasma HA levels led to a lack of sensitivity. In conclusion, this study demonstrates that Immunotech(®) EIA and LC-HRMS method could be used instead of RIA to assess plasma HA in human diagnostic use.

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“…In conclusion, besides a slight underestimation of HA concentrations measured by LC-HRMS, LC-HRMS gave particularly encouraging results, as a good correlation with RIA was found [18].…”

Section: Comparison Of Lc-hrms To Riamentioning

confidence: 70%

Section: Graphical Abstractmentioning

confidence: 99%

Section: Comparison Of the 3 Tested Assays To Riamentioning

confidence: 99%

See 1 more Smart Citation

Comparison of two enzymatic immunoassays, high resolution mass spectrometry method and radioimmunoassay for the quantification of human plasma histamine

Poli

Laurichesse

Rostan

et al. 2016

Journal of Pharmaceutical and Biomedical Analysis

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“…Calibrators were prepared by dilution of the histamine working standard solutions (Toronto Research Chemicals, Toronto, ON) with Tris BSA as a surrogate plasma matrix, as previously described (26) to concentrations ranging from 2.5 to 100 ng/mL. Calibration curves and negative control samples were prepared fresh for each quantitative assay.…”

Section: Histamine Plasma Concentration Determinationmentioning

confidence: 99%

Effect of Meperidine on Equine Blood Histamine, Tryptase, and Immunoglobulin-E Concentrations

et al. 2020

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Objectives: To evaluate changes in immunological parameters following subcutaneous (SC) and intramuscular (IM) administration of meperidine in horses through quantitative analysis of plasma tryptase, histamine, and IgE levels.Methods: Six adult horses were enrolled in a prospective randomized crossover design. Horses were administered one treatment per day, with a seven day washout period: (a) meperidine 1 mg/kg IM, saline 6 mL SC; (b) saline 6 mL IM, meperidine 1 mg/kg SC; (c) saline 6 mL SC, saline 6 mL IM. Blood samples were obtained for plasmatic histamine (baseline, 5, 10, 15, 30, and 60 min) via LC-MS/MS and plasmatic tryptase (baseline, 15, 30, 60, 120, and 240 min) quantification with enzyme-linked immunoabsorbent assays. Serum immunoglobulin E (IgE) concentrations prior to any meperidine treatment and 7–14 days following the first meperidine treatment were evaluated with enzyme-linked immunoabsorbent assays. Histamine and tryptase concentrations were evaluated with a mixed-effect analysis of variance. The levels of IgE at baseline (before the administration of the first dose of meperidine) were compared with the IgE values at 60 min following the second meperidine administration with the Paired t test. Biopsies of localized injection site reactions from subcutaneous meperidine administration were collected from two horses.Results: No statistically significant elevations from baseline in histamine (p = 0.595), tryptase (p = 0.836), or IgE (p = 0.844) were found in any of the horses in this study. There were no differences between treatment groups. Administration of SC meperidine caused a localized vasculitis and thrombosis with regional edema and hemorrhage.Conclusion: No evidence of anaphylactoid or anaphylactic type reactions occurred following IM or SC meperidine administration.

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“…14 LC coupled with tandem mass spectrometry (MS/MS) is also used for HA analysis, where HA- d 4 is used as an internal standard. 15,16 Non-separative methods such as enzyme immunoassaying 17 or radioimmunoassaying (RIA) 18 are also used. Of these methods, RIA is considered to be the gold standard.…”

Section: Introductionmentioning

confidence: 99%

A standard addition method to quantify histamine by reductive amination and hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry

Shen

Lin

Chen

et al. 2019

Eur J Mass Spectrom (Chichester)

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Histamine is an organic nitrogenous compound that acts as a neurotransmitter in the uterus, spinal cord, and brain and is involved in local immune responses. In this study, we developed a fast and simple derivatization method based on reductive amination that can be used to quantify histamine by hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry. Histamine isotope analogs were synthesized via reductive amination. Histamine was modified with H2-formaldehyde to form N-dimethylated histamine to act as a standard or with D2-formaldehyde to form N-dimethylated histamine- d4 to act as an internal standard. Using this method, we achieved a limit of detection of 3.6 ng/mL, a limit of quantification of 7.9 ng/mL, and a linear calibration curve with a coefficient of determination (R2) of 0.9987. Furthermore, the intra-day relative standard deviations ranged from 0.9% to 3.7% and the inter-day relative standard deviations ranged from 2.0% to 17.6%. After derivatization, N-dimethylated histamine showed 382.5% signal enhancement compared to unmodified histamine in mass spectrometry detection. To demonstrate the applicability of this method for biological samples, we utilized standard addition method to quantify histamine in fetal bovine serum and achieved a recovery of 86.7%.

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Histamine quantification in human plasma using high resolution accurate mass LC–MS technology

Cited by 7 publications

References 26 publications

Comparison of two enzymatic immunoassays, high resolution mass spectrometry method and radioimmunoassay for the quantification of human plasma histamine

Comparison of two enzymatic immunoassays, high resolution mass spectrometry method and radioimmunoassay for the quantification of human plasma histamine

Effect of Meperidine on Equine Blood Histamine, Tryptase, and Immunoglobulin-E Concentrations

A standard addition method to quantify histamine by reductive amination and hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry

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