High performance liquid chromatographic analysis and anticancer potential of Oplopanax horridus: Comparison of stem and berry extracts

Abstract

Oplopanax horridus or devil’s club is a herbal medicine distributed in North America. The constituents and pharmacological activities of O. horridus (OPH) are largely unknown. In this study, we assayed OPH stem and berry extracts using high performance liquid chromatography (HPLC). The anticancer potentials of extracts on different human cancer cell lines (SW-480, HCT-116, HT-29, MCF-7 and NSCLC) were determined by MTS method. The effect of stem extract on cancer cell cycle, expression of cyclin A, and apoptosis were assayed using flow cytometry. HPLC data showed that the composition of OPH stem extract is more complicated than the berry extract. The wavelength of maximum absorption of the major constituent in stem and berry is 196.0 nm and 201.9 nm, respectively. Compared to the berry extract, the stem extract showed significant potent antiproliferative effect on all the studied cell lines. The stem extract at 0.1 mg/ml arrested cancer cells in S- and G2/M-phases, and significantly induced expression of cyclin A. After treatment with 0.1 mg/ml of stem extract for 72 h, apoptotic cells were increased to 45.2%, while control was 9.6%. The cell cycle arrest and induction of apoptosis may play a critical role in cancer chemoprevention by Oplopanax horridus stem extract.

HPLC–MS analyses and bioactivities of novel chemicals in Devil’s club (Oplopanax horridus (Sm.) Miq.)

“…2, the whole extract of dried roots at concentrations of 0.1 and 0.2 mg/mL showed obvious anti-proliferative effect on the HT-29 cells, which were inhibited at 29.8% and 56.5%, respectively. The anti-cancer effect of the Devil's club extract against the HT-29 cells was also reported in another study, which indicated the stem of the Devil's club could inhibit 52% cell growth at the extract concentration of 0.25 mg/mL, and 84% at 0.5 mg/ml (Wang, Aung, Mehendale, Shoyama, & Yuan, 2010). Such differences in the anti-cancer activity of the Devil's club extract might result from the difference of chemical profiles between the root and stem, as well as the hydrolysis processing in sample preparation.…”

Antioxidant activity and cytotoxicity ofCyrtosperma johnstoniiextracts on drug sensitive and resistant leukemia and small cell lung carcinoma cells

“…Miq. (Araliaceae) effectively inhibit the proliferation of breast cancer cells via induction of cell cycle arrest (Wang et al, 2010). The extracts of Citrus grandis Osbeck (Rutaceae) and Onosma paniculatum Bur.…”

Chemical and pharmacological studies of Oplopanax horridus, a North American botanical

“…O. elatus is distributed throughout eastern Asia, including northeast China, Korea and the far east of Russia. Another species from this genus, O. japonicus , is distributed throughout Japan [5]. …”

“…The plant is a member of the family Araliaceae, the same family as more familiar plants such as American ginseng and is therefore occasionally referred to as “Alaskan ginseng” or “Pacific ginseng,” although they are in different genera [2-4]. There are three different species within Oplopanax , which include O. horridus, O. japonicus and O. elatus [5]. O. japonicus and O. elatus are distributed throughout Japan and eastern Asia, respectively [5], and there are beyond the scope of this article.…”

“…There are three different species within Oplopanax , which include O. horridus, O. japonicus and O. elatus [5]. O. japonicus and O. elatus are distributed throughout Japan and eastern Asia, respectively [5], and there are beyond the scope of this article.…”