Antioxidant activity and cytotoxicity of<i>Cyrtosperma johnstonii</i>extracts on drug sensitive and resistant leukemia and small cell lung carcinoma cells

Okonogi, Siriporn; Khonkarn, Ruttiros; Mankhetkorn, Samlee; Unger, Frank M.; Viernstein, Helmut

doi:10.3109/13880209.2012.729064

Cited by 12 publications

(13 citation statements)

References 25 publications

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“…Rutin present in ethanolic extract of aerial parts of Pupalia lappacea also exerted effects on leukemia K562 cells by functionalizing intrinsic pathway mediated apoptosis [ 25 ]. Extract of Cyrtosperma johnstonii contains rutin as a bioactive ingredient and has potent biological activity against small cell lung carcinoma cells as evidenced by cell cycle arrest and apoptosis [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

Rutin mediated targeting of signaling machinery in cancer cells

et al. 2014

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Progress in our understanding of molecular oncology has started to shed light on dysregulation of spatio-temporally controlled signaling pathways, inactivation of tumor suppressor genes, tumour and normal stem cell quiescence, overexpression of oncogenes, extracellular and stromal microenvironments, epigenetics and autophagy. Sequentially and characteristically it has been shown that cancer cells acquire the ability to escape from apoptotic cell death, proliferate uncontrollably, sustain angiogenesis and tactfully reconstitute intracellular pathways to avoid immune surveillance. We have attempted to provide a recent snapshot of most recent progress with emphasis on how rutin modulates wide ranging intracellular signaling cascades as evidenced by in-vitro and in-vivo research. It is worth describing that 'single-cell proteomics' analysis has further improved our understanding regarding intracellular signaling pathways frequently activated in cancer cells resistant to therapeutics and can provide biomarkers for cancer diagnosis and prognosis. Data obtained from preclinical studies will prove to be helpful for scientists to bridge basic and translational studies.

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Section: Introductionmentioning

confidence: 99%

Rutin mediated targeting of signaling machinery in cancer cells

et al. 2014

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“…Rutin possesses anti-inflammatory (inhibiting the mucosal content of platelet-activating factor, PAF) and vasoactive activity (diminishing capillary permeability) as well as antioxidant activity (scavenging of hydroxyl and superoxide radicals) (12). Also, anti-carcinogen activity of rutin against prostate, breast, lung and colon cancers was demonstrated (13,14).…”

Section: +mentioning

confidence: 99%

Modulatory effects of rutin on the expression of cytochrome P450s and antioxidant enzymes in human hepatoma cells

Karakurt

2016

Acta Pharmaceutica

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Expression of a drug and xenobiotic metabolizing enzymes, cytochrome P450s (CYPs), and antioxidant enzymes can be modulated by various factors. The flavonoid rutin was investigated for its anti-carcinogen and protective effects as well as modulatory action on CYPs and phase II enzymes in human hepatocellular carcinoma cells. Rutin inhibited proliferation of HEPG2 cells in a dose-dependent manner with the IC 50 value of 52.7 µmol L -1 and invasion of HEPG2 cells (21.6 %, p = 0.0018) and colony formation of those invaded cells (57.4 %, p < 0.0001). Rutin treatment also significantly increased early/late-stage apoptosis in HEPG2 cells (28.9 %, p < 0.001). Treatment by rutin significantly inhibited protein expressions of cytochrome P450-dependent CYP3A4 (75.3 %, p < 0.0001), elevated CYP1A1 enzymes (1.7-fold, p = 0.0084) and increased protein expressions of antioxidant and phase II reaction catalyzing enzymes, NQO1 (2.42-fold, p < 0.0001) and GSTP1 (2.03-fold, p < 0.0001). Besides, rutin treatment significantly inhibited mRNA expression of CYP3A4 (73.2 %, p=0.0014). Also, CYP1A1, NQO1 and GSTP1 mRNA expressions were significantly increased 2.77-fold (p = 0.029), 4.85-fold (p = 0.0051) and 9.84-fold (p < 0.0001), respectively.

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“…Three different extracts of S. grandiflora bark were prepared according to the method previously described (Okonogi et al. 2013 ). Briefly, the dried powder of S. grandiflora bark was macerated for 24 h in hexane, then filtered through a filter paper.…”

Section: Methodsmentioning

confidence: 99%

In vitro antibacterial activity and in vivo therapeutic effect of Sesbania grandiflora in bacterial infected silkworms

Anantaworasakul

Hamamoto

Sekimizu

et al. 2017

Pharmaceutical Biology

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Context: Antibiotic resistance is a serious problem worldwide. Searching for new potential agents is, therefore, essential. The bark of Sesbania grandiflora (L.) Pers. (Fabaceae) has been used in folk medicine against various diseases.Objective: To investigate the antibacterial activity of S. grandiflora bark and explore the therapeutic effect of the highest potent fraction.Materials and methods: Bacteria and healthy silkworms were exposed to three fractionated extracts (3.1–400 mg/mL) of S. grandiflora bark from hexane (HXF), chloroform (CFF), and ethyl acetate (EAF). The sets of bacteria were incubated at 37 °C while silkworms were kept at 27 °C for 24 h. To evaluate the therapeutic effect, silkworms infected with bacteria were exposed to the extracts (0.5–60 mg/mL) and incubated at 27 °C for 52 h. Qualitative analysis of the most potent extract was done using HPLC.Results: EAF showed the highest activity with MIC against methicillin resistant Staphylococcus aureus (MRSA) and vancomycin resistant enterococci (VRE) of 1.6 and 0.4 mg/mL, respectively, and against Gram-negative Escherichia coli and Pseudomonas aeruginosa of 6.2 and 3.1 mg/mL, respectively. It is nontoxic to silkworms with LC50 >400 mg/mL and has high therapeutic effect on infected silkworms with EC50 of 1.9 mg/mL. EAF consists of at least five major compounds, one of them is gallic acid. The activity of EAF is higher than the sum of individual activities of separated compounds.Discussion and conclusion: These results suggest that EAF is a promising antibacterial extract, suitable for further investigation in rodents infected with drug resistant bacteria.

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Antioxidant activity and cytotoxicity ofCyrtosperma johnstoniiextracts on drug sensitive and resistant leukemia and small cell lung carcinoma cells

Cited by 12 publications

References 25 publications

Rutin mediated targeting of signaling machinery in cancer cells

Rutin mediated targeting of signaling machinery in cancer cells

Modulatory effects of rutin on the expression of cytochrome P450s and antioxidant enzymes in human hepatoma cells

In vitro antibacterial activity and in vivo therapeutic effect of Sesbania grandiflora in bacterial infected silkworms

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