High performance aptamer affinity chromatography for single-step selective extraction and screening of basic protein lysozyme

Han, Bin; Zhao, Chao; Yin, Jie; Wang, Hailin

doi:10.1016/j.jchromb.2012.07.003

Cited by 62 publications

(37 citation statements)

References 52 publications

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“…Due to their high affinity and specificity, aptamers have gained an increasing utility in analytical, biosensing, diagnostic and therapeutic applications [6][7][8]. In analytical applications aptamers are used as affinity ligand or labelled probe to isolate, purify or analyse the aptamer-protein complexes [9][10][11][12][13][14]. Recently, we have reported an aptamer that targets Concanavalin A with an affinity of ∼172 nmole L −1 [15].…”

Section: Introductionmentioning

confidence: 99%

Development of an aptamer-affinity chromatography for efficient single step purification of Concanavalin A from Canavalia ensiformis

Ahirwar

Nahar

2015

Journal of Chromatography B

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Section: Introductionmentioning

confidence: 99%

Development of an aptamer-affinity chromatography for efficient single step purification of Concanavalin A from Canavalia ensiformis

Ahirwar

Nahar

2015

Journal of Chromatography B

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“…It must be emphasized that the most utilized monolith composition in bioseparation science is the E40/G60 composition. This is to ensure that there is a sufficient amount of epoxy functional groups for ligand functionalization without trading‐off the amount of cross‐linking, mechanical properties, and permeability …”

Section: Resultsmentioning

confidence: 99%

Thermogravimetric characterization of ex situ polymethacrylate (EDMA‐co‐GMA) monoliths

Acquah

Moy

Anwar³

et al. 2017

Can J Chem Eng

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The thermo-molecular mechanisms associated with free radical synthesis of polymethacrylate monoliths offer an effective pathway to tune their pore characteristics. In this work, thermogravimetric analyses were used for ex situ characterization of polymethacrylate monoliths synthesised from ethylene glycol dimethacrylate (EDMA) and glycidyl methacrylate (GMA). Apparent activation energies of the polymeric monoliths were determined by using Ozawa-Flynn-Wall (OFW) and Kissinger-Akahira-Sunose (KAS) isoconversional methods. The number of degradation stages for the polymers were observed to be as follows: homopolymeric GMA and EDMA (2 degradation stages each) and polymeric EDMA-co-GMA monoliths (3 degradation stages). The relationship between apparent activation energy and extent of conversion showed that the degradation of monoliths is based on a complex multi-step reaction rather than a single reaction model. Kinetic parameters showed that an increase in the composition of EDMA and GMA above 20 % significantly enhances the thermal stability of polymeric EDMA-co-GMA monoliths under elevated non-isothermal conditions. E40/G60 and E60/G40 monoliths were identified as the most thermally stable monoliths from the kinetic analyses.

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“…This SELEX modification allows fast generation of aptamers that recognize cell-type specific intracellular proteins. Target proteins can be further purified in native form by means of affinity chromatography on selected aptamers [70]. This approach can be useful for the analysis of purified enzymes, since fusion with affinity tags (GST, His, etc.)…”

Section: Limitations In Aptamer Application and Possible Sol Utionsmentioning

confidence: 99%

Aptamers: Problems, Solutions and Prospects

2013

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Aptamers are short single-stranded oligonucleotides that are capable of binding various molecules with high affinity and specificity. When the technology of aptamer selection was developed almost a quarter of a century ago, a suggestion was immediately put forward that it might be a revolutionary start into solving many problems associated with diagnostics and the therapy of diseases. However, multiple attempts to use aptamers in practice, although sometimes successful, have been generally much less efficient than had been expected initially. This review is mostly devoted not to the successful use of aptamers but to the problems impeding the widespread application of aptamers in diagnostics and therapy, as well as to approaches that could considerably expand the range of aptamer application.

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High performance aptamer affinity chromatography for single-step selective extraction and screening of basic protein lysozyme

Cited by 62 publications

References 52 publications

Development of an aptamer-affinity chromatography for efficient single step purification of Concanavalin A from Canavalia ensiformis

Development of an aptamer-affinity chromatography for efficient single step purification of Concanavalin A from Canavalia ensiformis

Thermogravimetric characterization of ex situ polymethacrylate (EDMA‐co‐GMA) monoliths

Aptamers: Problems, Solutions and Prospects

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