Development of an aptamer-affinity chromatography for efficient single step purification of Concanavalin A from Canavalia ensiformis

The synthesis of (Z)-4-oxo-4-(arylamino)but-2-enoic acids derivatives and determination of their inhibition properties against human carbonic anhydrase I and II isoenzymes

“…Due to its ability to enrich selective targets, affinity chromatography has remained a mainstay technique in separation chemistry [69][70][71][72] . In this study, both hCA I and II isoenzymes were purified by Sepharose-4B-L-tyrosine-sulfanilamide affinity chromatography [73][74][75][76] .…”

Chromatographic Immunoassays: Strategies and Recent Developments in The Analysis of Drugs and Biological Agents

“…Aptamers have been used as 'antibody mimics' or alternatives to antibodies in some chromatographic binding assays [17][18][19][20]. These binding agents consist of single-stranded DNA or RNA sequences that are often 10 to 100 nucleotides in length and that have the ability to form highly ordered 3D structures [19].…”

“…Aptamers are prepared through a selection and amplification process known as SELEX (i.e., the 'systematic evolution of ligands by exponential enrichment'), which can be used to generate specific binding agents with relatively high affinities for a particular target [17,18]. These agents have been used to bind targets that have included drugs, peptides, proteins and cells [17][18][19][20]. It is possible to easily modify the structure of an aptamer to include a label or to immobilize the aptamer to a support [18,19].…”

“…It is possible to easily modify the structure of an aptamer to include a label or to immobilize the aptamer to a support [18,19]. In addition to being used in biosensors [17,18], aptamers have been employed in chromatography for both the purification of proteins [20] and in various forms of chromatographic binding assays [19].…”

“…One possible area of future research is in the types of binding agents that can be used in chromatographic immunoassays and related methods. This is expected to include the more extensive use of aptamers [17][18][19][20] and MIPs [21][22][23][24][25]46,47] as alternatives to antibodies. It is also anticipated that there will be more applications in chromatographic immunoassays that make use of binding agents such as bispecific antibodies, single-chain antibody fragments and 'nanobodies' [6,16,80,107].…”