Chromatographic Immunoassays: Strategies and Recent Developments in The Analysis of Drugs and Biological Agents

Matsuda, Ryan; Rodriguez, Elliott; Suresh, D.; Hage, David S.

doi:10.4155/bio.15.206

Cited by 23 publications

(15 citation statements)

References 111 publications

(338 reference statements)

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“…13 There are several types of methods that fall in this category. 23,24 One of these is the simultaneous injection format, in which a mixture of the target and a fixed amount of a labeled analog of this target are injected together onto a column that contains a small amount of antibodies for the target. Due to competition between the labeled analog and the target for the immobilized antibodies, the amount of labeled analog that is non-retained will increase as the concentration of target in the sample is increased.…”

Section: Affinity Methods In Hplc Using Antibodiesmentioning

confidence: 99%

High performance affinity chromatography and related separation methods for the analysis of biological and pharmaceutical agents

Zhang

Rodriguez

et al. 2018

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The last few decades have witnessed the development of many high-performance separation methods that use biologically related binding agents. The combination of HPLC with these binding agents results in a technique known as high performance affinity chromatography (HPAC). This review will discuss the general principles of HPAC and related techniques, with an emphasis on their use for the analysis of biological compounds and pharmaceutical agents. Various types of binding agents for these methods will be considered, including antibodies, immunoglobulin-binding proteins, aptamers, enzymes, lectins, transport proteins, lipids, and carbohydrates. Formats that will be discussed for these methods range from the direct detection of an analyte to indirect detection based on chromatographic immunoassays, as well as schemes based on analyte extraction or depletion, post-column detection, and multi-column systems. The use of biological agents in HPLC for chiral separations will also be considered, along with the use of HPAC as a tool to screen or study biological interactions. Various examples will be presented to illustrate these approaches and their applications in fields such as biochemistry, clinical chemistry, and pharmaceutical research.

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Section: Affinity Methods In Hplc Using Antibodiesmentioning

confidence: 99%

High performance affinity chromatography and related separation methods for the analysis of biological and pharmaceutical agents

Zhang

Rodriguez

et al. 2018

Analyst

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“…IAC is a special type of bioaffinity chromatography in which the stationary phase is an antibody or antibody‐related reagent [, ]. This method can be employed for the purification or isolation of a given target compound (e.g.…”

Section: Binding Agents and Applications Of Amcmentioning

confidence: 99%

“…This method can be employed for the purification or isolation of a given target compound (e.g. a method sometimes referred to as “immunoextraction”) or as a means for the direct or indirect analysis of this compound . Antibodies have been useful as binding agents in traditional immunoaffinity chromatography and AMC‐based methods because of the wide range of targets for which they can be used, along with the high affinity and selectivity that they often have for these targets [, ].…”

Section: Binding Agents and Applications Of Amcmentioning

confidence: 99%

Affinity monolith chromatography: A review of general principles and applications

et al. 2017

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Affinity monolith chromatography, or AMC, is a liquid chromatographic method in which the support is a monolith and the stationary phase is a biological binding agent or related mimic. AMC has become popular for the isolation of biochemicals, for the measurement of various analytes, and for studying biological interactions. This review will examine the principles and applications of AMC. The materials that have been used to prepare AMC columns will be discussed, which have included various organic polymers, silica, agarose and cryogels. Immobilization schemes that have been used in AMC will also be considered. Various binding agents and applications that have been reported for AMC will then be described. These applications will include the use of AMC for bioaffinity chromatography, immunoaffinity chromatography, dye-ligand affinity chromatography, and immobilized metal-ion affinity chromatography. The use of AMC with chiral stationary phases and as a tool to characterize biological interactions will also be examined.

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“…These techniques rely on the selective and strong binding between an antibody and its target to provide assays that can often be used directly, and with minimal pretreatment steps, with complex samples such as serum or plasma [2,3]. In these methods, a column is used that contains one component of an immunoassay, making it possible to combine the binding selectivity and strength of antibodies with the precision, ease of automation, and speed of modern liquid chromatography [2][3][4][5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Development of a microcolumn one-site immunometric assay for a protein biomarker: Analysis of alpha1-acid glycoprotein

Zhang

Lott

Clarke

et al. 2020

Journal of Chromatography A

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This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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Chromatographic Immunoassays: Strategies and Recent Developments in The Analysis of Drugs and Biological Agents

Cited by 23 publications

References 111 publications

High performance affinity chromatography and related separation methods for the analysis of biological and pharmaceutical agents

High performance affinity chromatography and related separation methods for the analysis of biological and pharmaceutical agents

Affinity monolith chromatography: A review of general principles and applications

Development of a microcolumn one-site immunometric assay for a protein biomarker: Analysis of alpha1-acid glycoprotein

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