Affinity monolith chromatography: A review of general principles and applications

Abstract

Affinity monolith chromatography, or AMC, is a liquid chromatographic method in which the support is a monolith and the stationary phase is a biological binding agent or related mimic. AMC has become popular for the isolation of biochemicals, for the measurement of various analytes, and for studying biological interactions. This review will examine the principles and applications of AMC. The materials that have been used to prepare AMC columns will be discussed, which have included various organic polymers, silica, agarose and cryogels. Immobilization schemes that have been used in AMC will also be considered. Various binding agents and applications that have been reported for AMC will then be described. These applications will include the use of AMC for bioaffinity chromatography, immunoaffinity chromatography, dye-ligand affinity chromatography, and immobilized metal-ion affinity chromatography. The use of AMC with chiral stationary phases and as a tool to characterize biological interactions will also be examined.

Cryostructuring of Polymeric Systems. 55. Retrospective View on the More than 40 Years of Studies Performed in the A.N.Nesmeyanov Institute of Organoelement Compounds with Respect of the Cryostructuring Processes in Polymeric Systems

“…However, a more extensive development had a biotechnological direction related to the application of various wide-pore cryogels and cryostructurates in the bioseparation processes, i.e., upon the specific detection, isolation, and purification of the diverse biological molecules and bioparticles (protein bodies, plasmids, viruses, cell organelles, and whole cells) [ 5 , 12 , 13 , 90 , 93 , 94 , 95 , 98 , 112 , 113 , 114 , 115 , 116 , 117 , 118 , 119 , 120 , 121 , 122 , 123 , 124 , 125 , 126 , 127 , 128 , 129 , 130 , 131 , 132 , 133 , 134 , 135 , 136 , 137 , 138 , 139 , 140 , 141 ]. Among the numerous experimental studies, there are two frequently-quoted pioneering publications [ 339 , 340 ] that served as a real basis for further development of new materials and processes in the bioseparation field.…”

Polysaccharide-based chromatographic adsorbents for virus purification and viral clearance

“…For example, quaternary amine-functionalized polymethacrylate monoliths (CIM (Convective Interaction Media)® QA, Bia Separations, Villach, Austria) have been extensively applied to virus [154][155][156][157][158] and VLP [159,160] purification in recent years. Some monoliths based on PS, in particular AG, AG-chitosan composites or AG/chitosan-hybrids, have been implemented in AFC columns for purification of biological targets [161] with no reference to viral contaminants. In the work by Fernandes et al [162] -an exception, AdV-5 was successfully bound to and eluted from a chitosan-PVA monolith functionalized with a Q ligand.…”

Immobilization and Purification of Enzymes With the Novel Affinity Tag ChBD-AB From Chitinolyticbacter meiyuanensis SYBC-H1

“…Nowadays, a variety of chromatography methods are widely applied in various occasions where protein purification or immobilization are required. Thus, by fusing the coding sequence of the interest protein with different tag sequences, which have high affinity to a ligand, these protein can be efficiently purified for identification and characterization (Li et al, 2017).…”