Polysaccharide-based chromatographic adsorbents for virus purification and viral clearance

Junter, Guy-Alain; Lebrun, Laurent

doi:10.1016/j.jpha.2020.01.002

Cited by 31 publications

(22 citation statements)

References 238 publications

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“…Chromatography is a key technology in purification protocols for the main biotherapeutic products offering scalability, purity, high recovery rates, and reproducibility. [ 73 ] The classic resin media were originally designed for protein purification; thus, the large hydrodynamic radius of the LV raised some technical constraints. The use of beads often restricts access to the binding sites, resulting in small dynamic binding capacities.…”

Section: Scalable Methods For LV Downstream Processingmentioning

confidence: 99%

“…[22,24,70,72] In the size exclusion chromatography (SEC), viral vectors are excluded from the internal pores of the packing material while other biomolecules migrate slowly as they are more likely retained in the pores. [4,73] A maximum MWCO of 750 kDa (equivalent to 50 nm) is commonly used to prevent titer loss. [22] This method enables the efficient removal of small-sized particles with well-reported recoveries, from 50% to 80%.…”

Section: Polishing Formulation and Sterilizationmentioning

confidence: 99%

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Advances in Lentivirus Purification

Moreira

Cavaco

Faria

et al. 2020

Biotechnology Journal

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Lentiviral vectors (LVs) have been increasingly used as a tool for gene and cell therapies since they can stably integrate the genome in dividing and nondividing cells. LV production and purification processes have evolved substantially over the last decades. However, the increasing demands for higher quantities with more restrictive purity requirements are stimulating the development of novel materials and strategies to supply the market with LV in a cost-effective manner. A detailed review of each downstream process unit operation is performed, limitations, strengths, and potential outcomes being covered. Currently, the majority of large-scale LV manufacturing processes are still based on adherent cell culture, although it is known that the industry is migrating fast to suspension cultures. Regarding the purification strategy, it consists of batch chromatography and membrane technology. Nevertheless, new solutions are being created to improve the current production schemes and expand its clinical use.

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Section: Scalable Methods For LV Downstream Processingmentioning

confidence: 99%

Section: Polishing Formulation and Sterilizationmentioning

confidence: 99%

Advances in Lentivirus Purification

Moreira

Cavaco

Faria

et al. 2020

Biotechnology Journal

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show abstract

“…Currently, membrane technology has been combined with catalytic electrospun [ 79 ] and electrocoagulation [ 80 ] for virus capture, control and remediation from water at lab scale experiments. There are also approaches that focus in the adsorption of the virus through cell culture [ 81 ] and polysaccharide-based resins [ 82 ]. However, protocols during drinking water treatment focused on disinfecting from SARS-CoV-2 are missing [ 83 ].…”

Section: Perspectives For the Wastewater Treatment Post-covid-19mentioning

confidence: 99%

Approaches applied to detect SARS-CoV-2 in wastewater and perspectives post-COVID-19

Cervantes-Avilés

Moreno‐Andrade

Carrillo‐Reyes

2021

Journal of Water Process Engineering

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“…Antithrombin III, blood coagulation factors like FIX, FVIII‐von Willebrand complexes, lipoproteins, DNA binding proteins, and various types of viruses have been purified using both resins. [ 17–19 ] Antithrombin III, fibrinonectin, and vitamin K dependent clotting factors/inhibitors have similar plassma‐protein binding capacity and selectivity for heparin type resins and polysaccharide‐based resins with immobilized sulfate groups. Thus, it appears that the specific binding of vitamin‐K dependent proteins is not dependent on the carbohydrate backbone of the polyelectrolyte chain, rather on something else that is yet to be identified.…”

Section: Introductionmentioning

confidence: 99%

“…As a working hypothesis, we therefore assumed that the salt tolerance and higher affinity for plasma proteins are interrelated and dependent on the presence of this sulfate group. [ 17–22 ]…”

Section: Introductionmentioning

confidence: 99%

Salt‐tolerant cation exchanger‐containing sulfate groups as a viable alternative for mixed‐mode type and heparin‐based affinity resins

Begić

Pečenković

Gajdošik

et al. 2021

Biotechnology Journal

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Ion‐exchange chromatography is still one of the most popular protein separation techniques. Before chromatographic separation, the high salt concentration in various samples necessitates additional steps. Therefore, low salt tolerance of ion‐exchange resins is a drawback that needs to be addressed. Herein, the differences in salt tolerance and hydrophobicity of strong cation‐exchange TOYOPEARL resins of sulfonium and sulfate‐types were investigated. Despite only a minor structural difference, differences in selectivity and salt tolerance between the sulfate and sulfonic groups were detected. In silico calculations were also carried out for model substances representing the sulfonium and sulfate groups, wherein significant differences in hydrophobicity was observed. These experiments confirmed the hypothesis that the salt tolerance, higher affinity, and selectivity for certain vitamin K dependent clotting factors are interrelated and dependent on the presence of the sulfate group. Separation of clotting factor IX from the prothrombin complex concentrate further to confirmed the affinity for these proteins. The results show that the use of only a resin with the sulfate ligand and not with the sulfonic acid ligand allows for a facile and rapid separation of clotting factor IX and other vitamin K dependent clotting factors.

show abstract

Polysaccharide-based chromatographic adsorbents for virus purification and viral clearance

Cited by 31 publications

References 238 publications

Advances in Lentivirus Purification

Advances in Lentivirus Purification

Approaches applied to detect SARS-CoV-2 in wastewater and perspectives post-COVID-19

Salt‐tolerant cation exchanger‐containing sulfate groups as a viable alternative for mixed‐mode type and heparin‐based affinity resins

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