High mannose-specific lectin (KAA-2) from the red alga Kappaphycus alvarezii potently inhibits influenza virus infection in a strain-independent manner

Abstract

The carbohydrate binding profile of the red algal lectin KAA-2 from Kappaphycus alvarezii was evaluated by a centrifugal ultrafiltration-HPLC method using pyridylaminated oligosaccharides. KAA-2 bound exclusively to high mannose type N-glycans, but not to other glycans such as complex type, hybrid type, or the pentasaccharide core of N-glycans. This lectin exhibited a preference for an exposed α1-3 Man on a D2 arm in a similar manner to Eucheuma serra agglutinin (ESA-2), which shows various biological activities, such as anti-HIV and anti-carcinogenic activity. We tested the anti-influenza virus activity of KAA-2 against various strains including the recent pandemic H1N1-2009 influenza virus. KAA-2 inhibited infection of various influenza strains with EC50s of low nanomolar levels. Immunofluorescence microscopy using an anti-influenza antibody demonstrated that the antiviral activity of KAA-2 was exerted by interference with virus entry into host cells. This mechanism was further confirmed by the evidence of direct binding of KAA-2 to a viral envelope protein, hemagglutinin (HA), using an ELISA assay. These results indicate that this lectin would be useful as a novel antiviral reagent for the prevention of infection.

High Mannose-binding Lectin with Preference for the Cluster of α1–2-Mannose from the Green Alga Boodlea coacta Is a Potent Entry Inhibitor of HIV-1 and Influenza Viruses

“…Immunofluorescence Microscopy-Immunofluorescence staining was performed to visualize and evaluate BCA inhibition of influenza virus infection as described previously (29). Briefly, MDCK cells grown on cover glass were infected with A/Udorn/72 at a multiplicity of infection of ϳ0.001 infectious particles per cell, in the presence or absence 1 M BCA in DMEM containing 10 g/ml trypsin.…”

Penapisan Senyawa Hemagglutinin dari Makroalga Asal Pantai Binuangeun, Banten, Indonesia

“…Kemampuan lektin untuk mengikat karbohidrat secara spesifik pada permukaan sel (glikokonjugat), memungkinkan protein ini dapat dikembangkan untuk berbagai tujuan, termasuk untuk deteksi dan identifikasi kelompok darah dan mikroorganisme, determinasi karbohidrat dalam suatu larutan, juga untuk pemurnian glikoprotein (Van Damme, 2014). Karena kemampuannya untuk mengikat karbohidrat di permukaan sel secara spesifik tersebut, lektin juga mempunyai banyak peran penting dalam interaksi antara host-patogen, komunikasi sel-sel, mengenali dan mengikat karbohidrat yang kemudian menghasilkan efek fungsional untuk menginduksi apoptosis, berperan sebagai antibiotik, antiinflammatory, anti-HIV serta anti agregasi platelet (Liao et al, 2003;Dinh et al, 2011;Sato et al, 2011;Hamid et al, 2013;Takebe et al, 2013). Secara alami, lektin banyak ditemukan pada hampir semua makhluk hidup: tumbuhan, jamur, bakteri, virus, hewan (vertebrata dan avertebrata).…”

Biological Applications of Plants and Algae Lectins: An Overview

“…Concerning the antiviral effects, a lectin named KAA-2 from the red marine algal Kappaphycus alvarezii (specific to high mannose type N-glycans) showed antiviral action against H1N1 virus [117]. The red alga lectin from Griffithsia sp.…”