High local genetic diversity of canine parvovirus from Ecuador

Aldaz, Jaime; García-Díaz, Juan Ramón; Calleros, Lucía; Sosa, Katia; Iraola, Gregorio; Marandino, Ana; Hernández, Martı́n; Panzera, Yanina; Pérez, Ruben

doi:10.1016/j.vetmic.2013.06.012

Cited by 38 publications

(38 citation statements)

References 38 publications

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“…Nandi et al [32] reported presence of CPV-2c type from North India, but it was not until 2015 (present study) that CPV-2c type was first isolated in India (Jabalpur, Madhya Pradesh, and Central India), which clearly indicated slow but steady spread of CPV-2c type in Indian dog population. It is a matter of concern as CPV-2c type is often associated with severe infections even in adult dogs regardless of their vaccination status [33] and replaces the other predominant CPV types within few years of its emergence [34][35][36].…”

Section: Discussionmentioning

confidence: 99%

Full-length VP2 gene analysis of canine parvovirus reveals emergence of newer variants in India

Nookala¹,

Mukhopadhyay²,

Sivaprakasam³

et al. 2016

Acta Microbiologica et Immunologica Hungarica

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The canine parvovirus (CPV) infection is a highly contagious and serious enteric disease of dogs with high fatality rate. The present study was taken up to characterize the full-length viral polypeptide 2 (VP2) gene of CPV of Indian origin along with the commercially available vaccines. The faecal samples from parvovirus suspected dogs were collected from various states of India for screening by PCR assay and 66.29% of samples were found positive. Six CPV-2a, three CPV-2b, and one CPV-2c types were identified by sequence analysis. Several unique and existing mutations have been noticed in CPV types analyzed indicating emergence of newer variants of CPV in India. The phylogenetic analysis revealed that all the field CPV types were grouped in different subclades within two main clades, but away from the commercial vaccine strains. CPV-2b and CPV-2c types with unique mutations were found to be establishing in India apart from the prevailing CPV-2a type. Mutations and the positive selection of the mutants were found to be the major mechanism of emergence and evolution of parvovirus. Therefore, the incorporation of local strain in the vaccine formulation may be considered for effective control of CPV infections in India.

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Section: Discussionmentioning

confidence: 99%

Full-length VP2 gene analysis of canine parvovirus reveals emergence of newer variants in India

Nookala¹,

Mukhopadhyay²,

Sivaprakasam³

et al. 2016

Acta Microbiologica et Immunologica Hungarica

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“…Approximately 20 years after the emergence of the CPV-2c, this has been found mainly in South American (Calderón et al, 2011;P erez et al, 2012;Pinto et al, 2012;Aldaz et al, 2013) and European countries Sutton et al, 2013). However, that variant has not been detected in Oceania.…”

Section: The Worldwide Distribution Of Antigenic Cpv-2 Variants Spreamentioning

confidence: 99%

Canine parvovirus: the worldwide occurrence of antigenic variants

Miranda

Thompson

2016

Journal of General Virology

171

198

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“…Epidemiologic survey of fecal samples of dogs from Western European showed that the original CPV-2 was replaced by its variants, being the CPV-2c the most prevalent in Italy and Germany and at high rates in Spain and France (Decaro & Buonavoglia 2012). In Ecuador, CPV--2c was the most prevalent variant detected (54.7%) in rectal swabs of puppies (Aldaz et al 2013). Molecular epidemiology in stool samples of dogs with diarrhea from China showed CPV-2c in 14.74% of the samples (Geng et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Epidemiological, clinical and pathological features of canine parvovirus 2c infection in dogs from southern Brazil

et al. 2018

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Canine parvovirus type 2c (CPV-2c) emerged in Europe in the early 2000’s and rapidly spread out worldwide. Clinical and molecular data have demonstrated its circulation in Brazilian dogs, yet detailed descriptions of cases are still lacking. This article describes the epidemiological, clinical and pathological features of 24 cases of CPV-2c-associated disease in dogs submitted to veterinary clinics and laboratory diagnosis in southern Brazil (2014-2016). Most affected dogs presented signs/lesions suggestive of parvovirus enteritis: diarrhea, vomiting, hyperemia and hemorrhage of the serous membrane of the small intestine, diffuse segmental granulation, atrophy of the villi, necrosis and fusion of crypts, squamous metaplasia and epithelial syncytia. A number of cases presented features divergent from the classical presentations, including a wide variation in the color of feces (reddish and/or yellowish, light-brownish, orange-brown and brownish), involvement of adults (4/24) and vaccinated dogs (12/24), extensive involvement of the small intestine (8/20) and the presence of pulmonary edema (7/24) and convulsions (3/24). Feces and intestinal fragments submitted to PCR for the CPV-2 VP2 gene and to virus isolation in cell culture yielded positive results in 100% and 58.3% (14/24) of the cases, respectively. Nucleotide sequencing revealed a high nucleotide identity in VP2 (99.4 to 100%) and a consistent mutation at amino acid 426 (asparagine to glutamic acid), considered a signature of CPV-2c. These results confirm the involvement of CPV-2c in the described cases and demonstrate the importance of CPV-2c infection among Brazilian dogs, calling attention of veterinarians to correctly diagnose the disease, mainly considering the frequent atypical presentations.

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High local genetic diversity of canine parvovirus from Ecuador

Cited by 38 publications

References 38 publications

Full-length VP2 gene analysis of canine parvovirus reveals emergence of newer variants in India

Full-length VP2 gene analysis of canine parvovirus reveals emergence of newer variants in India

Canine parvovirus: the worldwide occurrence of antigenic variants

Epidemiological, clinical and pathological features of canine parvovirus 2c infection in dogs from southern Brazil

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