High genetic diversity of the VP2 gene of a canine parvovirus strain detected in a domestic cat

Abstract

This study reports the detection of co-infection by multiple CPV variants and the high genetic complexity of a CPV-2 strain detected in a domestic cat. The CPV variants selected by cloning the VP2 gene were sequenced, and genetic diversity and selection pressure were investigated. Comparison of the nucleotide sequences has evidenced 10 different viral populations, and, in the same animal, more CPV variants coexist. Our analysis excludes the possibility that the recombination events took place during infection and that negative selection acted on the VP2 gene. These findings confirm that CPV-2 shows high genetic heterogeneity resembling the quasispecies found in RNA viruses.

Comparative molecular analysis of strains of the Aleutian Disease Virus isolated from farmed and wild mink

“…The high differentiation in the case of parvoviruses is also confirmed by research on CPV-2, in the case of which numerous genetic variants may attack a single individual [19]. In the presented study, marked differences were observed between the wild and farmed groups.…”

Genetic analysis of feline panleukopenia viruses from cats with gastroenteritis

“…Residue 297 is located in the antigenic region close to epitope B and substitutions at this position may be responsible for changes in antigenicity of CPV-2 variants (Truyen, 2006). Analogously, a change at position 440 (TAA) has been described for Italian, Indian, Korean and American CPV-2 isolates (Battilani et al, 2002;Chinchkar et al, 2006;Kang et al, 2008;Kapil et al, 2007) and for a clone from a mixed CPV-2 population detected in a domestic cat from Italy (Battilani et al, 2006b). As for the FPLV strains, that change is a consequence of a first-base mutation (A1318G).…”

Canine parvovirus: the worldwide occurrence of antigenic variants

“…CPV-2a and -2b viruses have been also isolated in feline cells in vitro and in domestic cats in vivo from Japan, USA, Taiwan and Vietnam (Truyen et al, 1995(Truyen et al, , 1996aMochizuki et al, 1996;Ikeda et al, 2000). Diseased cats have also been detected with CPV-2c (Miranda et al, 2014) or co-infections by multiple CPV variants (Battilani et al, 2006) or mixed infections with FPV and CVP-2-like (Url et al, 2003;Battilani et al, 2011). Some studies have also reported the presence of CPV-2 variants in faecal and bone marrow samples of healthy cats (Clegg et al, 2012;Haynes & Holloway, 2012).…”