High-frequency synthetic apomixis in hybrid rice

Vernet, Aurore; Meynard, Donaldo; Lian, Qichao; Mieulet, Delphine; Gibert, Olivier; Bissah, Matilda; Rivallan, Ronan; Autran, Daphné; Leblanc, Olivier; Meunier, Anne Cécile; Frouin, Julien; Taillebois, James E.; Shankle, Kyle; Khanday, Imtiyaz; Mercier, Raphaël; Sundaresan, Venkatesan; Guiderdoni, Emmanuel

doi:10.1038/s41467-022-35679-3

Cited by 48 publications

(32 citation statements)

References 60 publications

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“…By contrast, in Arabidopsis, embryogenesis was not found to be dependent on the male allele expression of AtBBM ; instead AtBBM was expressed exclusively in female maternal tissues before fertilization, and from both parental alleles in zygotes (Chen et al ., 2022). Moreover, whereas Os BBM1 expression in the egg cell induces parthenogenesis at frequencies as high as 30% in rice (Khanday et al ., 2019) or even > 95% (Vernet et al ., 2022), At BBM expression in dicots results in very low frequencies of parthenogenesis (< 1.5%) (Chen et al ., 2022). These contrasting observations indicate important differences between monocot and dicot plants, both in the regulation of BBM gene expression and in the roles of the parental genomes, for the initiation of ZE.…”

Section: Discussionmentioning

confidence: 99%

Somatic embryo initiation by rice BABY BOOM1 involves activation of zygote‐expressed auxin biosynthesis genes

2023

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Summary Plant embryogenesis results from the fusion of male and female gametes but can also be induced in somatic cells. The molecular pathways for embryo initiation are poorly understood, especially in monocots. In rice, the male gamete expressed BABY BOOM1 (OsBBM1) transcription factor functions as an embryogenic trigger in the zygote and can also promote somatic embryogenesis when ectopically expressed in somatic tissues. We used gene editing, transcriptome profiling, and chromatin immunoprecipitation to determine the molecular players involved in embryo initiation downstream of OsBBM1. We identify OsYUCCA (OsYUC) auxin biosynthesis genes as direct targets of OsBBM1. Unexpectedly, these OsYUC targets in zygotes are expressed only from the maternal genome, whereas the paternal genome exclusively provides functional OsBBM1 to initiate embryogenesis. Induction of somatic embryogenesis by exogenous auxin requires OsBBM genes and downstream OsYUC targets. Ectopic OsBBM1 initiates somatic embryogenesis without exogenous auxins but requires functional OsYUC genes. Thus, an OsBBM‐OsYUC module is a key player for both somatic and zygotic embryogenesis in rice. Zygotic embryo initiation involves a partnership of male and female genomes, through which paternal OsBBM1 activates maternal OsYUC genes. In somatic embryogenesis, exogenous auxin triggers OsBBM1 expression, which then activates endogenous auxin biosynthesis OsYUC genes.

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Section: Discussionmentioning

confidence: 99%

Somatic embryo initiation by rice BABY BOOM1 involves activation of zygote‐expressed auxin biosynthesis genes

2023

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“…In rice, simultaneous silencing of three meiosis‐related genes, SPO11‐1 , REC8 , and OSD1 , induced the transition from meiotic to mitotic processes in oocytes (Mieulet et al, 2016). Editing the meiosis‐ or fertilization‐related genes SPO11‐1 ‐ REC8 ‐ OSD1 or REC8 ‐ PAIR1 ‐ OSD1 ‐ MTL achieved apomixis in hybrid rice, as did simultaneously inducing the MiMe mutation via ectopic expression of OsBBM1 or OsBBM4 in the oocyte (Wang et al, 2019; Vernet et al, 2022; Wei et al, 2023). Recently, specific expression of the dandelion PAR in the MiMe system generated apomictic materials with high seed‐setting rates throughout successive generations (Song et al, 2023).…”

Section: The Development Of One‐line Rice Breedingmentioning

confidence: 99%

A historical review of hybrid rice breeding

Zheng,

Wei,

Cheng

et al. 2024

JIPB

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The development of germplasm resources and progress in breeding methods have led to steady increases in rice (Oryza sativa) yield and quality. Three milestones in the recent history of rice breeding have contributed to these increases: dwarf rice breeding, hybrid rice breeding, and super rice breeding. On the occasion of the 50th anniversary of the success of three‐line hybrid rice, we highlight important scientific discoveries in rice breeding that were made by Chinese scientists and summarize the broader history of this field. In addition, we discuss the strategies that could be used in the future to further optimize rice breeding in the hope that China will continue to be a leader in the international rice breeding arena.This article is protected by copyright. All rights reserved.

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“…BABY BOOM (BBM), an AP2 transcription factor family protein, can initiate maternal parthenogenic embryogenesis without fertilization and leads to haploid formation in rice, maize, millet and Arabidopsis [ 15–18 ]. By combining a Meiosis instead of Mitosis ( MiMe ) system with BBM , researchers have shown that hybrid vigor can be efficiently fixed across generations through synthetic apomixis in rice [ 19 , 20 ]. However, ectopic expression of the BBM gene in egg cells is a prerequisite, so BBM- mediated parthenogenic haploids are transgenic and have limited application in plant breeding.…”

Section: Introductionmentioning

confidence: 99%

Creation of a watermelon haploid inducer line via ClDMP3-mediated single fertilization of the central cell

Chen

et al. 2023

Horticulture Research

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Creating doubled haploids is one of the most efficient breeding methods in modern agriculture. Radiated pollen grains could induce haploids in cucurbit crops, which leads to a hypothesis that radiated pollen results in the preferential fertilization of the central cell over the egg cell was the reason of haploid formation. Disruption of DMP gene caused single fertilization of the central cell, which further induced haploids formation in plants. In the present study, a detailed method of creating watermelon haploid inducer line via ClDMP3 mutation was described. cldmp3 mutant induced haploids in different watermelon genotypes at the rates up to 1.12%. The generated haploids have been confirmed by different methods, including fluorescent marker, flow cytometry, molecular marker and immuno-staining assisted verifications. The haploid inducer created by this method will greatly advance the traditional watermelon breeding in future.

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High-frequency synthetic apomixis in hybrid rice

Cited by 48 publications

References 60 publications

Somatic embryo initiation by rice BABY BOOM1 involves activation of zygote‐expressed auxin biosynthesis genes

Somatic embryo initiation by rice BABY BOOM1 involves activation of zygote‐expressed auxin biosynthesis genes

A historical review of hybrid rice breeding

Creation of a watermelon haploid inducer line via ClDMP3-mediated single fertilization of the central cell

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