High frequency shoot formation of yellow passion fruit (Passiflora edulis F. flavicarpa) via thin Cell Layer (TCL) Technology

Nhựt, Dương Tấn; Khiet, Bui Le Thanh; Thi, Nguyen; Thuy, Dang Thi Thu; Duy, Nguyễn Xuân; Hải, Nguyễn Thị Thanh; Huyen, Phan Xuan

doi:10.1007/978-1-4020-6352-7_38

Cited by 19 publications

(17 citation statements)

References 19 publications

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“…Considering the organogenesis-based regeneration system currently prevailing in passion fruit (Vieira and Carneiro 2004;Vieira et al 2005;Nhut et al 2007;Zerbini et al 2008), the present work may be a useful foundation for extending regeneration via somatic embryogenesis to other Passiflora species. Furthermore, this technique provides an alternative choice for explants and regeneration that may result in more efficient propagation rates and more reproducible and efficient transformation protocols for the genus Passiflora.…”

Section: Resultsmentioning

confidence: 95%

“…Since then, an increasing number of reports on tissue culture-based techniques applied to the genus have been published for a number of Passiflora species (Vieira and Carneiro 2004;Passos and Bernacci 2005;Nhut et al 2007;Zerbini et al 2008). Despite the fact that several protocols for in vitro propagation and regeneration of Passiflora spp.…”

Section: Introductionmentioning

confidence: 98%

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A novel regeneration system for a wild passion fruit species (Passiflora cincinnata Mast.) based on somatic embryogenesis from mature zygotic embryos

Silva

Pinto

Guerra

et al. 2009

Plant Cell Tiss Organ Cult

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The objective of the present work was to induce somatic embryogenesis from zygotic embryos of Passiflora cincinnata Masters. Zygotic embryos formed calli on media with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and 4.5 lM benzyladenine (BA) after 30 days of in vitro culture. A concentration of 18.1 lM 2,4-D resulted in the largest number of somatic embryos. Embryogenic calli were yellowish and friable, forming whitish proembryogenic masses. Morphologically, embryogenic cells were small and had large nuclei and dense cytoplasm, whereas non-embryogenic cells were elongated, with small nuclei and less dense cytoplasm. Calli cultured under white light on basal Murashige and Skoog's medium with activated charcoal produced embryos in all developmental stages. There were differences among the treatments, with some leading to the production of calli with embryos and some only to callus formation. Some abnormalities were associated with somatic embryos, including fused axes, fused cotyledons and polycotyledonary embryos. Production of secondary somatic embryos occurred in the first cycle of primary embryo development. Secondary embryos differentiated from the surface of the protodermal layer of primary embryos with intense cell proliferation, successive mitotic divisions in the initial phase of embryoid development, and a vascular system formed with no connection to the parental tissue. This secondary embryogenic system of P. cincinnata is characterized by intense proliferation and maintenance of embryogenic competence after successive subcultures. This reproducible protocol opens new prospects for massive propagation and is an alternative to the current organogenesis-based transformation protocol.

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Section: Resultsmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 98%

A novel regeneration system for a wild passion fruit species (Passiflora cincinnata Mast.) based on somatic embryogenesis from mature zygotic embryos

Silva

Pinto

Guerra

et al. 2009

Plant Cell Tiss Organ Cult

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“…Exogenous PGRs can stimulate cell division, cell growth, and cell differentiation in the medium. So far, few studies have been reported in the field of direct regeneration and effects of different concentrations of PGRs on micropropagation of P. caerulea (Busilacchi et al, 2008;Ozarowski et al, 2013;Prithrivaj et al, 2015); there are also several reports on other species of this genus, such as P. foetida (Anand et al, 2012), Passiflora setacea (Vieira et al, 2014), P. edulis ) Nhut et al, 2007) and Passiflora cincinnata (Da Silva et al, 2011). Shoot proliferation protocol via cotyledonary nodes and shoot tip segments as explants presented in this study, has important advantages such as large-scale production in a short period of time.…”

Section: Effects Of Pgrs On Shoot Regenerationmentioning

confidence: 99%

In vitro shoot proliferation of Passiflora caerulea L. via cotyledonary node and shoot tip explants

Jafari¹,

Daneshvar²,

Lotfi³

2017

bta

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Passiflora caerulea L. is a herbaceous climber that belongs to the Passifloraceae family. One of the most important techniques used in plant biotechnology is tissue culture, which allows for the mass production of pathogenfree plants. Cotyledonary nodes have a great potential for shoot proliferation; however, to the best of our knowledge there are no reports regarding plant regeneration from cotyledonary nodes of P. caerulea. Therefore, this study aimed to evaluate the potential of two different types of explants (shoot tips and cotyledonary nodes) to obtain shoot multiplication of P. caerulea. Various concentrations of 6-benzylaminopurine (BAP) (0.5, 1, and 1.5 mg/l), 6-furfurylaminopurine (kinetin, KIN) (1 and 2 mg/l), and thidiazuron (TDZ) (0.25, 0.5, and 1 mg/l) in combination with indole butyric acid (IBA) were used in a completely randomized design, in three replications. The results showed that the highest percentage of regeneration frequency (90%) and a maximum number of shoots (8.86) in cotyledonary node explants were obtained on MS medium supplemented with 1.5 mg/l BAP along with 0.15 mg/l IBA. Furthermore, in the shoot tip explants, the percentage of regeneration rate (96.66%) and the highest number of shoots (9.86) were obtained in the above-mentioned medium. In rooting experiments, the maximum rooting percentage (90%) was obtained on MS medium containing 1 mg/l IBA. In vitro-raised plantlets were placed in pots and were stored in soil under room temperature for 20 to 30 days before planting, and it showed more than 90% survival rate. Based on our results, the protocol described in this study has a high potential to be used in the micropropagation of this valuable plant.

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“…Passiflora edulis Sims f. flavicarpa Degener, yellow passion fruit, is distinguished among other Passiflora species because it represents 90% of the commercial plantations of the genus. This species has been the subject of most studies involving in vitro culture of the genus, and has been extensively reviewed elsewhere (Vieira and Carneiro 2004;Nhut et al 2007;Zerbini et al 2008). P. cincinnata Masters is widely distributed throughout tropical America (Cunha and Barbosa 2002), and in some regions, it is considered as an invasive species due to its vigorous growth.…”

Section: Introductionmentioning

confidence: 98%

Ethylene and polyamine production patterns during in vitro shoot organogenesis of two passion fruit species as affected by polyamines and their inhibitor

Dias

Santa–Catarina

Ribeiro

et al. 2009

Plant Cell Tiss Organ Cult

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Levels of ethylene and polyamines (PAs) were measured during organogenesis of hypocotyl explants of two species of passion fruit (Passiflora cincinnata Masters and Passiflora edulis Sims f. flavicarpa Degener 'FB-100') to better understand the relationships of these regulators and their influence on cell differentiation and morphogenesis. Moreover, histological investigation of shoot ontogenesis was conducted to characterize the different events involved in cell redifferentiation and regulation of PA and ethylene levels. A delay was observed in morphogenic responses of P. edulis f. flavicarpa as compared to P. cincinnata, and these changes coincided with production of elevated levels of polyamine and ethylene levels. During differentiation, cells showed high rates of expansion and elongation, and high ethylene levels were associated with high PA levels, suggesting that the two biosynthesis pathways were highly regulated. Moreover, their interaction might be an important factor for determining cell differentiation. The addition of PAs to the culture medium did not promote organogenesis; however, the incorporation of the PA inhibitor methylglyoxal bisguanylhydrazone in the culture medium reduced shoot bud differentiation, suggesting the need to maintaining a minimum level of PAs for morphogenic events to take place.

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High frequency shoot formation of yellow passion fruit (Passiflora edulis F. flavicarpa) via thin Cell Layer (TCL) Technology

Cited by 19 publications

References 19 publications

A novel regeneration system for a wild passion fruit species (Passiflora cincinnata Mast.) based on somatic embryogenesis from mature zygotic embryos

A novel regeneration system for a wild passion fruit species (Passiflora cincinnata Mast.) based on somatic embryogenesis from mature zygotic embryos

In vitro shoot proliferation of Passiflora caerulea L. via cotyledonary node and shoot tip explants

Ethylene and polyamine production patterns during in vitro shoot organogenesis of two passion fruit species as affected by polyamines and their inhibitor

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