In vitro shoot proliferation of Passiflora caerulea L. via cotyledonary node and shoot tip explants

Abstract

Passiflora caerulea L. is a herbaceous climber that belongs to the Passifloraceae family. One of the most important techniques used in plant biotechnology is tissue culture, which allows for the mass production of pathogenfree plants. Cotyledonary nodes have a great potential for shoot proliferation; however, to the best of our knowledge there are no reports regarding plant regeneration from cotyledonary nodes of P. caerulea. Therefore, this study aimed to evaluate the potential of two different types of explants (shoot tips and cotyledonary nodes) to obtain shoot multiplication of P. caerulea. Various concentrations of 6-benzylaminopurine (BAP) (0.5, 1, and 1.5 mg/l), 6-furfurylaminopurine (kinetin, KIN) (1 and 2 mg/l), and thidiazuron (TDZ) (0.25, 0.5, and 1 mg/l) in combination with indole butyric acid (IBA) were used in a completely randomized design, in three replications. The results showed that the highest percentage of regeneration frequency (90%) and a maximum number of shoots (8.86) in cotyledonary node explants were obtained on MS medium supplemented with 1.5 mg/l BAP along with 0.15 mg/l IBA. Furthermore, in the shoot tip explants, the percentage of regeneration rate (96.66%) and the highest number of shoots (9.86) were obtained in the above-mentioned medium. In rooting experiments, the maximum rooting percentage (90%) was obtained on MS medium containing 1 mg/l IBA. In vitro-raised plantlets were placed in pots and were stored in soil under room temperature for 20 to 30 days before planting, and it showed more than 90% survival rate. Based on our results, the protocol described in this study has a high potential to be used in the micropropagation of this valuable plant.

In Vitro Adventitious Shoot Regeneration through Direct and Indirect Organogenesis from Seedling-derived Hypocotyl Segments of Ficus religiosa L.: An Important Medicinal Plant

“…The effect of different PGRs on callus induction. In vitro plant regeneration from different parts of in vitro-grown seedlings has received notable attention, and many researchers have used various parts of in vitrogrown seedling as explants (Jafari et al, 2017;Mali and Chavan, 2016;Niazian et al, 2017;Phulwaria et al, 2013;Singh et al, 2016) to improve the micropropagation method for many plant species. This study demonstrated the candidature of hypocotyl segments, which are obtained from axenic seedling, as a source of explant for in vitro regeneration of F. religiosa for the first time ( Fig.…”

“…There was much evidence which elucidated that determination of a suitable concentration and type of PGRs had a massive impact on the rate of successful regeneration of F. religiosa. Many studies proved the positive influence of cytokinin on cell division and shoot regeneration (Arab et al, 2014;Jafari et al, 2017;Gill, 2011, 2014). Hesami et al (2017a) and Siwach and Gill (2014) reported that TDZ is more effective than BAP on the number of shoot regeneration, whereas various studies take a radical point of view and alleged that BAP is more effective than TDZ (Deshpande et al, 1998;Hassan et al, 2009;.…”

Effects of thidiazuron on in vitro shoot regeneration of Morus alba

“…Rooting is a crucial step in the success of micropropagation (Jafari et al, 2017). Without an effective root system, plant acclimatization would be difficult, and the rate of plant propagation might be severely affected.…”

“…Kollmeier et al (2000) reported that callus formation, at the end of the microshoots, can impair vascular connections between roots and shoots and can also prevent the absorption of water and nutrients. To sustain plant's life and increase its efficacy in various environmental conditions, it is necessary to gradually change rhizosphere to enable plants to withstand stress (Gaspar et al, 1996;Jafari et al, 2017). After 3 weeks, the plantlets were subcultured in the hor-mone-free medium for 2 weeks in the presence of the complete concentration of mineral elements.…”

“…Tissue and organ cultures have been widely used for the mass production of various plants (Hesami and Daneshvar, 2016a;Hesami et al, 2019b). Regarding the application of tissue culture techniques for the propagation of ornamental and medicinal plants, most studies have focused on two goals: mass production of plants and production of disease-free plants Jafari et al, 2017;Jafari et al, 2016). Indirect shoot organogenesis, as one of the methods of plant tissue culture, has some disadvantages: it is a time-consuming process and creates wide somaclonal varieties with morphological abnormalities .…”