High-fat diet-induced obesity Rat model: a comparison between Wistar and Sprague-Dawley Rat

Abstract

In the past decades, obesity and associated metabolic complications have reached epidemic proportions. For the study of these pathologies, a number of animal models have been developed. However, a direct comparison between Wistar and Sprague-Dawley (SD) Rat as models of high-fat (HF) diet-induced obesity has not been adequately evaluated so far. Wistar and SD rats were assigned for 2 experimental groups for 17 weeks: standard (St) and high-fat (HF) diet groups. To assess some of the features of the metabolic syndrome, oral glucose tolerance tests, systolic blood pressure measurements and blood biochemical analysis were performed throughout the study. The gut microbiota composition of the animals of each group was evaluated at the end of the study by real-time PCR. HF diet increased weight gain, body fat mass, mesenteric adipocyte's size, adiponectin and leptin plasma levels and decreased oral glucose tolerance in both Wistar and SD rats. However, the majority of these effects were more pronounced or earlier detected in Wistar rats. The gut microbiota of SD rats was less abundant in Bacteroides and Prevotella but richer in Bifidobacterium and Lactobacillus comparatively to the gut microbiota of Wistar rats. Nevertheless, the modulation of the gut microbiota by HF diet was similar in both strains, except for Clostridium leptum that was only reduced in Wistar rats fed with HF diet. In conclusion, both Wistar and SD Rat can be used as models of HF diet-induced obesity although the metabolic effects caused by HF diet seemed to be more pronounced in Wistar Rat. Differences in the gut microbial ecology may account for the worsened metabolic scenario observed in Wistar Rat.

Colonic luminal microbiota and bacterial metabolite composition in pregnant Huanjiang mini-pigs: effects of food composition at different times of pregnancy

“…Another study showed that long-term consumption of diets rich in carbohydrates can increase the abundance of Prevotella 43. In addition, a study showed that Prevotella decreased in rats fed a high fat diet44.…”

PEMBERIAN MINYAK IKAN LELE (Clarias gariepinus) TERHADAP BOBOT BADAN DAN PERUBAHAN HISTOPATOLOGI HATI, GINJAL DAN OTAK TIKUS GALUR Sprague dawley YANG DIBERI PAKAN HIPERKOLESTEROLEMIA

“…Hal ini sesuai dengan penelitian yang dilakukan Nikasa et al (2016) yang menunjukkan bahwa diet tinggi kolesterol secara signifikan meningkatkan bobot badan tikus. Beberapa penelitian serupa menunjukkan bahwa tikus yang diberikan pakan tinggi lemak bobot badannya akan meningkat secara signifikan (Davidson et al 2010;Chien et al 2016;Marques et al 2016). Pada minggu ketiga dan keempat mulai diberikan intervensi berdasarkan kelompok perlakuan.…”

Therapeutic Impact of Berries (Morus Alba and Morus Rubra) Fruit Extract in the Regression of High-Fat Diet-Induced Cardiac Dysfunction in Rats

“…However, obesity may produce a fail on adiponectin signaling (resistance of diponectin). However, Marques et al [16] showed that adiponectin plays a role in the decrease of insulin sensitivity happened by HFD and illustrated that hyperglycemia initiated by the HFD was associated with linked by a decrease in gene expression of adiponectin in the adipose tissue while did not cause a reduction in the serum levels of adiponectin, speculating that there is a compensative influence of the other depots of fat on serum levels of adiponectin. Previously, it El-Baz et al…”