High‐fat diet induced obesity and age influence the telomere shelterin complex and telomerase gene expression in mouse adipose tissue

Bloom, Samuel I.; Tuluca, Andrei; Ives, Stephen J.; Reynolds, Thomas H.

doi:10.14814/phy2.14461

Cited by 11 publications

(9 citation statements)

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“…Telomere shortening is associated with cardiovascular events, stroke, type 2 diabetes, and a higher mortality rate ( 10 ). Several lifestyle-related factors are associated with telomere shortening like smoking ( 11 – 13 ), alcohol intake even in minor amounts ( 14 , 15 ), low physical activity ( 16 , 17 ), and high-fat diets ( 18 , 19 ) that trigger telomere shortening. Obesity is a well-known risk factor for many age-related comorbidities and directly increases the risk of oxidative stress and inflammation ( 20 , 21 ).…”

Section: Introductionmentioning

confidence: 99%

RETRACTED: Obesity Accelerates Leukocyte Telomere Length Shortening in Apparently Healthy Adults: A Meta-Analysis

et al. 2022

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BackgroundShorter telomere length is associated with numerous comorbidities. Several studies have investigated the role of obesity in telomere shortening. In the current systematic review and meta-analysis, we summarized the results of studies that evaluated the association between obesity and telomere length.MethodsA systematic search from Scopus, PubMed, Embase, and ProQuest electronic databases up to 19 March 2021 without language restriction was performed and after data extraction and screening, 19 manuscripts were eligible to be included in the final meta-synthesis.ResultsThe highest category of telomere length was associated with an approximate 0.75 kg/m2 reduction in body mass index (BMI; WMD = −0.75 kg/m2; CI = −1.19, −0.31; p < 0.001; I2 = 99.4%). Moreover, overweight/obese individuals had 0.036 kbp shorter telomere length compared with non-overweight/obese adults (WMD = −0.036; CI = −0.05, −0.02; p = 0.030; I2 = 100%). According to the results of subgroupings, continent, age, and sample size could be possible sources of heterogeneity.ConclusionFrom the results, it was clear that obesity was associated with shorter telomere length. Because of the observational design of included studies, the causality inference of results should be done with caution; thus, further longitudinal studies are warranted for better inference of causal association.

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Section: Introductionmentioning

confidence: 99%

RETRACTED: Obesity Accelerates Leukocyte Telomere Length Shortening in Apparently Healthy Adults: A Meta-Analysis

et al. 2022

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“…Animals were housed at the Skidmore College animal facility on a 12:12 light:dark cycle and had access to food and water ad libitum. After initial acclimatization (1 week), the mice were randomized into either a high‐fat diet (HFD; 60% kcal from fat) or a low‐fat diet (LFD; 10% kcal from fat) group and consumed their respective diet for 4.5 months (Test Diets; Bloom et al., 2020; Reynolds et al., 2019; Shortreed et al., 2009; Trajcevski et al., 2013). Using a randomized controlled design, during the final 8 weeks of the diet intervention, the groups were subsequently randomized to receive either vehicle (VEH, drinking water) or succinic acid (SA; 0.75 mg/ml in drinking water; Ferro, Carbone, Marzouk, et al., 2017; Ferro, Carbone, Zhang, et al., 2017; Figure 1).…”

Section: Methodsmentioning

confidence: 99%

“…Upon the completion of the in vivo testing, mice were anesthetized with a 1:1:1 mixture of PromAce, ketamine hydrochloride, and xylazine by intraperitoneal injection (1.5 ml/ kg; Bloom et al, 2020 ). The soleus muscle, an oxidative skeletal muscle, was dissected and removed for subsequent determination of in vitro mitochondrial respiratory capacity.…”

Section: Tissue Harvestmentioning

confidence: 99%

The effect of succinic acid on the metabolic profile in high‐fat diet‐induced obesity and insulin resistance

et al. 2020

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Obesity, insulin resistance, and poor metabolic profile are hallmarks of a high‐fat diet (HFD), highlighting the need to understand underlying mechanisms. Therefore, we sought to determine the effect of succinic acid (SA) on metabolism in high‐fat diet (HFD)‐induced obesity. Animals were randomly assigned to either low‐fat diet (LFD) or a high‐fat diet (HFD). Mice consumed their respective diets for 4.5 months and then assigned to the following groups: (LFD)+vehicle, LFD + SA (0.75 mg/ml), HFD + vehicle, or HFD + SA. Body weight (BW), food, and water intake, were tracked weekly. After 6 weeks, insulin, glucose, and pyruvate tolerance tests were completed, and spontaneous physical activity was assessed. Epididymal white adipose tissue (EWAT) mass and in vitro measurements of oxidative skeletal muscle (soleus) respiration were obtained. Expectedly, the HFD increased BW and EWAT mass, and reduced glucose and insulin tolerance. SA significantly reduced EWAT mass, more so in HFD ( p < .05), but had no effect on any in vivo measurements (BW, insulin, glucose, or pyruvate tolerance, nor physical activity, all p > .05). A significant ( p < .05) interaction was observed between mitochondrial respiration and treatment, where SA increased respiration, likely owed to greater mitochondrial content, as assessed by complex IV activity in both LFD and HFD. In HFD‐induced obesity, coupled with insulin desensitization, we found no favorable effect of succinic acid on glucose regulation, though adiposity was attenuated. In oxidative skeletal muscle, there was a tendency for increased respiratory capacity, likely owed to greater mitochondrial content, suggestive of a succinic acid‐induced mitochondrial biogenesis.

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“…Similarly, it has been observed that adiposity is associated with high plasma levels of lipoperoxidation due to an increase in 8-epi-prostaglandin F2α (8-epi-PGF2α), which leads to the appearance of complications, such as insulin resistance [ 220 ]. At the transcriptional level, it also has a negative impact, since it influences the gene expression of the shelterin complex, thereby altering the function of telomeres and triggering the inflammatory process and predisposition to chronic diseases [ 221 ].…”

Section: Components Of the Mets And Its Relationship With Oxidative Stress And Telomeric Lengthmentioning

confidence: 99%

“…In aged animals, TERT gene expression is negatively correlated with weight, while TNF-α and MCP-1 have a positive correlation, suggesting that age and weight are factors that impact telomerase expression. This can generate an alteration in the function of telomeres in adipose tissue, thereby predisposing to an inflammatory state and age-related chronic diseases [ 221 ].…”

Section: Telomere Length and Inflammation In The Metsmentioning

confidence: 99%

Telomere Length and Oxidative Stress and Its Relation with Metabolic Syndrome Components in the Aging

Gavia-García

Rosado-Pérez

Arista-Ugalde

et al. 2021

Biology

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A great amount of scientific evidence supports that Oxidative Stress (OxS) can contribute to telomeric attrition and also plays an important role in the development of certain age-related diseases, among them the metabolic syndrome (MetS), which is characterised by clinical and biochemical alterations such as obesity, dyslipidaemia, arterial hypertension, hyperglycaemia, and insulin resistance, all of which are considered as risk factors for type 2 diabetes mellitus (T2DM) and cardiovascular diseases, which are associated in turn with an increase of OxS. In this sense, we review scientific evidence that supports the association between OxS with telomere length (TL) dynamics and the relationship with MetS components in aging. It was analysed whether each MetS component affects the telomere length separately or if they all affect it together. Likewise, this review provides a summary of the structure and function of telomeres and telomerase, the mechanisms of telomeric DNA repair, how telomere length may influence the fate of cells or be linked to inflammation and the development of age-related diseases, and finally, how the lifestyles can affect telomere length.

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High‐fat diet induced obesity and age influence the telomere shelterin complex and telomerase gene expression in mouse adipose tissue

Cited by 11 publications

References 59 publications

RETRACTED: Obesity Accelerates Leukocyte Telomere Length Shortening in Apparently Healthy Adults: A Meta-Analysis

RETRACTED: Obesity Accelerates Leukocyte Telomere Length Shortening in Apparently Healthy Adults: A Meta-Analysis

The effect of succinic acid on the metabolic profile in high‐fat diet‐induced obesity and insulin resistance

Telomere Length and Oxidative Stress and Its Relation with Metabolic Syndrome Components in the Aging

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