High-efficiency and low-cost production of cadaverine from a permeabilized-cell bioconversion by a Lysine-induced engineered Escherichia coli

Rui, Jinqiu; You, Shengping; Zheng, Yunxin; Wang, Chengyu; Gao, Yingtong; Zhang, Wei; Qi, Wei; Su, Rongxin; He, Zhimin

doi:10.1016/j.biortech.2020.122844

Cited by 30 publications

(6 citation statements)

References 35 publications

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“… 50 The non-ionic detergent Brij 56 was also confirmed to increase the yield of cadaverine by Hafnia alvei . 104 Rui et al 105 have studied the permeabilized cells of engineered strain E. coli BL21 (Pcad–CadA) by 35% (v/v) ethanol, which can produce 205 g L −1 cadaverine after 20 h by using the industrial materials in 2 L fermenter ( Fig. 7B ).…”

Section: Process Intensification For Cadaverine Productionmentioning

confidence: 99%

Green chemical and biological synthesis of cadaverine: recent development and challenges

Huang

Zhan-ling

et al. 2021

RSC Adv.

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Section: Process Intensification For Cadaverine Productionmentioning

confidence: 99%

Green chemical and biological synthesis of cadaverine: recent development and challenges

Huang

Zhan-ling

et al. 2021

RSC Adv.

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“…Engineered strain BL21-P cad -CadA (E. coli BL21 harboring plasmid with cad promoter and CadA) was treated with ethanol to solve mass-transfer limitation, and high cadaverine concentration of 205 g/L with yield of 92% was achieved. 71 The costs of industrial materials (yeast power, tryptone, sodium chloride, seed fermentation broth, inducer, antibiotic, ethanol, buffer, coenzyme instead of chemical reagents) were only $2.78, 11 times less than lab reagents $30.88 by liter-scale integrated strategy, which is hopefully to be a promising and efficient candidate for industrial scale production of cadaverine. Tween 40 also could be an additive to increase fluxes as raising membrane permeability.…”

Section: Bacteriamentioning

confidence: 99%

Cellular Engineering and Biocatalysis Strategies toward Sustainable Cadaverine Production: State of the Art and Perspectives

Liu

et al. 2021

ACS Sustainable Chem. Eng.

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Cadaverine, a diamine monomer, broadly exists in living organisms participating in metabolism processes. As a promising substitute of 1,6-diaminohexane, cadaverine can polymerize with dicarboxylic acids and yield biobased polyamides, polyamide (PA) 5×, showing ecofriendly and excellent mechanical properties in the fields of electronics, automobile, material, storage, and others. Due to the increasing attentions on environment problems, biopolyamide is expected to be an ideal and green material to substitute conventional chemistry polyamide. This review summarizes the properties, potential applications, and production strategies about cadaverine and mainly focuses on the recent developments by cellular engineering Escherichia coli and Corynebacterium glutamicum as main workhorses. In addition, the key enzyme lysine decarboxylase decorated by means of immobilization and mutation to efficiently catalyze lysine and its catalysis mechanisms are also discussed. In order to achieve industrial applications, the indispensable steps of separation and purification are described as well and perspectives for cadaverine manufacturing from renewable resources are further provided.

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“…Cadaverine has been produced via fermentation or whole cell bioconversion processes by engineered Corynebacterium glutamicum or Escherichia coli [ 9 , 10 ] . For example, by expressing lysine decarboxylases (LDCs) originated from different organism sources in E. coli , whole-cell biocatalysts for the efficient production of cadaverine from lysine have been obtained, and the highest level of cadaverine (205 g/L) was achieved from 400 g/L lysine with a molar yield of 92.1% [ 11 ]. For the fermentative production of cadaverine from renewable carbon sources, such as glucose, multiple metabolic engineering strategies have been studied and applied to engineer C. glutamicum or E. coli to improve production, such as overexpressing or engineering LDC [ 12 , 13 ]; enhancing metabolic flux towards the precursor lysine by overexpressing key genes or deleting an undesired competing pathway; eliminating the pathway of byproduct accumulation; transporter engineering to improve cadaverine export or increase the intracellular lysine level; and improving cofactor supply [ 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Ameliorating end-product inhibition to improve cadaverine production in engineered Escherichia coli and its application in the synthesis of bio-based diisocyanates

Wang

Guo

Wang

et al. 2021

Synthetic and Systems Biotechnology

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Cadaverine is an important C5 platform chemical with a wide range of industrial applications. However, the cadaverine inhibition on the fermenting strain limited its industrial efficiency of the strain. In this study, we report an engineered Escherichia coli strain with high cadaverine productivity that was generated by developing a robust host coupled with metabolic engineering to mitigate cadaverine inhibition. First, a lysine producing E. coli was treated with a combination of radiation (ultraviolet and visible spectrum) and ARTP (atmospheric and room temperature plasma) mutagenesis to obtain a robust host with high cadaverine tolerance. Three mutant targets including HokD, PhnI and PuuR are identified for improved cadaverine tolerance. Further transcriptome analysis suggested that cadaverine suppressed the synthesis of ATP and lysine precursor. Accordingly, the related genes involved in glycolysis and lysine precursor, as well as cadaverine exporter was engineered to release the cadaverine inhibition. The final engineered strain was fed-batch cultured and a titer of 58.7 g/L cadaverine was achieved with a yield of 0.396 g/g, both of which were the highest level reported to date in E. coli . The bio-based cadaverine was purified to >99.6% purity, and successfully used for the synthesis of polyurethane precursor 1,5-pentamethylene diisocyanate (PDI) through the approach of carbamate decomposition.

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High-efficiency and low-cost production of cadaverine from a permeabilized-cell bioconversion by a Lysine-induced engineered Escherichia coli

Cited by 30 publications

References 35 publications

Green chemical and biological synthesis of cadaverine: recent development and challenges

Green chemical and biological synthesis of cadaverine: recent development and challenges

Cellular Engineering and Biocatalysis Strategies toward Sustainable Cadaverine Production: State of the Art and Perspectives

Ameliorating end-product inhibition to improve cadaverine production in engineered Escherichia coli and its application in the synthesis of bio-based diisocyanates

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