High-efficiency and high-fidelity ssDNA circularisation via the pairing of five 3′-terminal bases to assist LR-LAMP for the genotyping of single-nucleotide polymorphisms

Abstract

The poor fidelity of T4 DNA ligase has always limited the simple detection of single-nucleotide polymorphisms (SNPs) and is only applicable to several special SNP types. This study developed a...

Host Range and Loop-Mediated Isothermal Amplification Detection of Globisporangium sylvaticum from Guizhou, China

“…Since the development of the LAMP method in 2000, it has been widely applied for the detection of various pathogens and has proven to be a rapid, simple, and efficient method with excellent practical value [22][23][24]. The ingenious design of the dumbbell-shaped DNA does not require thermal denaturation during the reaction and enables rapid, continuous, and specific amplification at a low cost.…”

OWL2: a molecular beacon-based nanostructure for highly selective detection of single-nucleotide variations in folded nucleic acids

“…10 PCR has an astounding range of applications from probe-based real-time PCR to post-amplification product analysis but relies on expensive instrumentation with precise temperature control for SNV differentiation. [11][12][13][14] Hybridization assays utilizing peptide nucleic acid and locked nucleic acid probes, 15,16 cycling probe technology, 17 TaqMan, 18 and Molecular Beacon (MB) 19 probes all suffer from the affinity/selectivity dilemma, which declares that tight binding of a probe to an analyte is associated with low selectivity. 20,21 Recent advances in SNV detection include ratio sensing via depletion of wild-type (WT) target, 22 programmable DNAzymes, 23 the use of CRISPR/Cas systems in conjunction with hybridization chain reactions, 24 and detection via lateral flow dipsticks after recombinase polymerase amplification with altered primers.…”

A fully integrated nucleic acid analysis system for multiplex detection of genetic polymorphisms related to folic acid metabolism

“…Among these methods, LAMP shows high sensitivity and specificity, but is rarely used for detecting SNPs due to the complex primer design. Some studies have helped in improving LAMP amplification to achieve SNP detection, 25 but still require sophisticated optical instruments to detect the fluorescent signals. At present, no LAMP-amplification technology is used to detect SNPs associated with folic acid metabolism.…”