OWL2: a molecular beacon-based nanostructure for highly selective detection of single-nucleotide variations in folded nucleic acids

Mueller, Brittany L.; Liberman, Mark J.; Kolpashchikov, Dmitry M.

doi:10.1039/d2nr05590b

Cited by 1 publication

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“…Recently, to address these issues, Mueller et al. developed a multicomponent hybridization probe, which employed two target binding arms to tightly bind and unwind a folded target sequence, and two sequence-specific strands to bind the target and stem-loop molecular beacon (MB) probe to open it to generate fluorescence signals, enabling it to differentiate SNVs in folded analytes in the temperature range of 5–38°C [ 72 ]. Further, through investigation of the thermodynamic basis of the narrow temperature intervals for the traditional linear ssDNA hybridization probes and MB probes, Guo et al.…”

Section: Homogeneous Snv Assays In the Test Tubementioning

confidence: 99%

Recent advances in enzyme-free and enzyme-mediated single-nucleotide variation assay in vitro

Xiong,

Liu,

Deng

et al. 2024

National Science Review

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Single-nucleotide variants (SNVs) are the most common type variation of sequence alterations at a specific location in the genome, thus involving significant clinical and biological information. The assay of SNVs has engaged great awareness, because many genome-wide association studies demonstrated that SNVs are highly associated with serious human diseases. Moreover, the investigation of SNV expression level in single cells are capable of visualizing genetic information and revealing the complexity and heterogeneity of single-nucleotide mutations-related diseases. Thus, developing SNV assay approaches in vitro, particularly in single cells, is in increasing demand. In this review, we summarized recent progress in the enzyme-free and enzyme-mediated strategies enabling SNV assay transition from sensing interface to the test tube and single cells, which will potentially delve deeper into the knowledge of SNV functions and disease associations, as well as discover new pathways to diagnose and treat diseases based on individual genetic profiles. The leap of SNV assay achievements will motivate observation and measurement genetic variations in single cells, even within living organisms, delve into the knowledge of SNV functions and disease associations, as well as open up entirely new avenues to diagnose and treat diseases based on individual genetic profiles.

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Section: Homogeneous Snv Assays In the Test Tubementioning

confidence: 99%

Recent advances in enzyme-free and enzyme-mediated single-nucleotide variation assay in vitro

Xiong,

Liu,

Deng

et al. 2024

National Science Review

View full text Add to dashboard Cite

show abstract

OWL2: a molecular beacon-based nanostructure for highly selective detection of single-nucleotide variations in folded nucleic acids

Abstract: Hybridization probes have been used in the detection of specific nucleic acids for the last 50 years. Desipte the great significance in diagnistics and extensive efforts in developing, the challenges...

Cited by 1 publication

References 44 publications

Recent advances in enzyme-free and enzyme-mediated single-nucleotide variation assay in vitro

Recent advances in enzyme-free and enzyme-mediated single-nucleotide variation assay in vitro

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