High doses of olive leaf extract induce liver changes in mice

Arantes‐Rodrigues, Regina; Henriques, Andreia; Pires, Maria dos Anjos; Colaço, Bruno; Calado, Ana; Rema, Paulo; Colaço, Aura; Fernandes, Telma; Cruz, P Luis Felipe. De La; Lopes, Carlos; Gonçalves, Lio; Vilela, Sérgio M. F.; Pedrosa, Tatiana do Nascimento; Peixoto, Francisco; Oliveira, Paula A.

doi:10.1016/j.fct.2011.05.010

Cited by 24 publications

(17 citation statements)

References 19 publications

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“…These results are interesting, as some of the signs of toxicity commonly associated with natural compounds are mortality and behavioral changes. 37,39 So, laurel extract administration at this dose did not cause toxicity. The body mass of HPV +/− animals treated with laurel extract was significantly lower than the remaining groups.…”

Section: Discussionmentioning

confidence: 66%

Laurus nobilis(laurel) aqueous leaf extract's toxicological and anti-tumor activities in HPV16-transgenic mice

et al. 2018

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Cancers induced by human papillomavirus (HPV) infection remain a significant public health threat, fueling the study of new therapies. Laurel (Laurus nobilis) compounds and extracts recently showed in vitro activity against HPV-transformed cell lines. This work aims to evaluate the in vivo efficacy and hepatic toxicity of a laurel extract in a transgenic mouse model of HPV16-induced cancer. The extract was administered in drinking water (20 mg per animal per day) for three consecutive weeks, using four experimental groups (n = 10) (group I: HPV16-/- without treatment, group II: treated HPV16-/-, group III: HPV16+/- without treatment and group IV: treated HPV16+/-). Following the treatment period, animals were sacrificed and skin samples were used to classify skin lesions histologically. Toxicological parameters included hematological and biochemical blood markers, splenic and hepatic histology and hepatic oxidative stress. The extract did not prevent the progression of HPV16-induced cutaneous lesions in this model. The treated wild-type animals showed mild hepatitis, while transgenic animals suffered weight loss. However, there were no changes concerning hematological, biochemical and hepatic oxidative stress markers.

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Section: Discussionmentioning

confidence: 66%

Laurus nobilis(laurel) aqueous leaf extract's toxicological and anti-tumor activities in HPV16-transgenic mice

et al. 2018

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“…In addition, the 800 μg/ml concentration may be considered the lowest observed adverse effect level (LOAEL) for female rats. Biochemically defined, cholestase consists of altered serum constituents that are hyperbilirubinemia, bile acidemia, and elevated enzymes activity such as ALP and γ-GT [26,27]. In the present study, although there was a rise in the serum bilirubin level for the males, no other parameter was altered, while the females did not suffer alteration of any of these three parameters, excluding the presence of cholestasis.…”

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confidence: 46%

Preclinical Toxicity Evaluation of Quassia amara Extract with Potential Antimycobacterial Activity

Emerick¹

2012

JBABM

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This study aimed to evaluate the oral acute and sub chronic toxicity of Quassia amara (Q. amara) extract. For the acute toxicity study, six male and six female rats received 2000 and 5000 mg/kg Q. amara extract. Rats were observed for 14 days; after sacrifice, liver, kidneys, lung, spleen and heart were submitted to macroscopic analysis. No death occurred and just the liver presented significant difference compared to the control group. For subchronic toxicity, eight groups of 6 male and 6 female rats received 200, 400 and 800 μg/mL of extract of Q. amara. Prothrombin time, bilirubin, urea, creatinine and gamma glutamyl transpeptidase (γ-GT), aspartate transaminase (AST), alanine transaminase (ALT) and alkaline phosphatase (ALP) were used as markers of renal and hepatobiliary function, respectively. The male showed a slight rise in serum bilirubin and creatinine and the female slight rise of AST, ALT and ALP activities. The urinalyses (pH, density, glucose and leukocytes) showed no change relative to the control. The NOAEL of 35 mg/kg and RfD 0.35 mg/kg/day encourage us to isolate the active principles in order to discover which molecules of this extract showed better antimycobacterial activity.

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“…12 Furthermore, olive leaf contains triterpenes (oleanolic, ursolic and maslinic acid) with confirmed antihypertensive, anti-atherosclerotic and antioxidant activity on a Dahl salt-sensitive, insulin-resistant genetic model of hypertension. 19 It was also shown that feeding doses of 0.2-0.9 % of OLE to Wistar rats for a period of 6 weeks may induce hematological, biochemical as well as hepatocellular and renal abnormalities of experimental animals. 14,15 The beneficial properties of olive leaf are further enhanced by the good absorption of its phenolic constituents and their bioavailability, which is a necessary pre-condition for its bioactivity.…”

Section: Introductionmentioning

confidence: 99%

Effects of high dose olive leaf extract on haemodynamic and oxidative stress parameters in normotensive and spontaneously hypertensive rats

Dekanski¹,

Mihailović-Stanojević

Milanovic

et al. 2014

JSCS

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The antihypertensive activity of olive leaf extract (OLE), a natural antioxidant is recognized, but its influence on the cardiovascular system when administered in a high dose has not yet been investigated. The aim of the present study was to determine the acute effects of excessive intake of standardized OLE on blood pressure, heart rate and oxidative status in both spontaneously hypertensive rats and normotensive Wistar rats. The systolic arterial pressure and heart rate were measured using a tail-cuff and pneumatic pulse detector before and 60 and 120 min after intragastric OLE administration. The activities of catalase, glutathione peroxidase, superoxide dismutase (SOD) and glutathione reductase in erythrocytes, as well as lipid peroxidation in plasma (pTBARS) were measured spectrophotometrically at the same time points. A high-dose of OLE did not influence blood pressure, heart rate or pTBARS in normotensive rats, while the SOD, catalase and glutathione reductase activities were significantly increased. The same dose significantly decreased blood pressure in hypertensive rats, but increased the pTBARS and SOD activity. Excessive oral intake of OLE induced moderate hypotensive effects only in spontaneously hypertensive rats, suggesting the absence of harmful hemodynamic effects after an oral overdose in both rat strains. However, its pro-oxidative role when given in a high dose in hypertensive organisms should not be neglected.

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High doses of olive leaf extract induce liver changes in mice

Cited by 24 publications

References 19 publications

Laurus nobilis(laurel) aqueous leaf extract's toxicological and anti-tumor activities in HPV16-transgenic mice

Laurus nobilis(laurel) aqueous leaf extract's toxicological and anti-tumor activities in HPV16-transgenic mice

Preclinical Toxicity Evaluation of Quassia amara Extract with Potential Antimycobacterial Activity

Effects of high dose olive leaf extract on haemodynamic and oxidative stress parameters in normotensive and spontaneously hypertensive rats

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