Laurus nobilis(laurel) aqueous leaf extract's toxicological and anti-tumor activities in HPV16-transgenic mice

Abstract

Cancers induced by human papillomavirus (HPV) infection remain a significant public health threat, fueling the study of new therapies. Laurel (Laurus nobilis) compounds and extracts recently showed in vitro activity against HPV-transformed cell lines. This work aims to evaluate the in vivo efficacy and hepatic toxicity of a laurel extract in a transgenic mouse model of HPV16-induced cancer. The extract was administered in drinking water (20 mg per animal per day) for three consecutive weeks, using four experimental groups (n = 10) (group I: HPV16-/- without treatment, group II: treated HPV16-/-, group III: HPV16+/- without treatment and group IV: treated HPV16+/-). Following the treatment period, animals were sacrificed and skin samples were used to classify skin lesions histologically. Toxicological parameters included hematological and biochemical blood markers, splenic and hepatic histology and hepatic oxidative stress. The extract did not prevent the progression of HPV16-induced cutaneous lesions in this model. The treated wild-type animals showed mild hepatitis, while transgenic animals suffered weight loss. However, there were no changes concerning hematological, biochemical and hepatic oxidative stress markers.

Dietary Supplementation with the Red Seaweed Porphyra umbilicalis Protects against DNA Damage and Pre-Malignant Dysplastic Skin Lesions in HPV-Transgenic Mice

“…In consequence, this animal model develops multi-step lesions of the skin [21]. Our group and others have characterized these lesions and shown that their development may be blocked pharmacologically, using investigational and commercial drugs [20,22,28]. HPV16 causes cancers in mucosal surfaces such as the cervix rather than the skin, but skin lesions in this model closely follow the same histological and molecular pattern of multi-step carcinogenesis observed in cervical cancer [29,30].…”

“…The present study addresses, for the first time, the potential of P. umbilicalis as a functional food to block the development of HPV-induced pre-malignant dysplastic lesions through its incorporation into the diet of HPV16-transgenic (K14HPV16) mice [19]. These animals develop multi-step cutaneous lesions induced by the HPV16 oncogenes, from hyperplastic foci through dysplastic patches to invasive squamous cell carcinomas, and may be used to test chemopreventive strategies [20,21]. K14HPV16 mice also show a debilitating syndrome, characterized by systemic inflammation, stunted growth, and chronic hepatitis, which progresses to overt cachexia with age [22].…”

Component composition of essential oil shoots and leaves of Laurus nobilis L. Ukrainian origian

“…In the world scientific literature there is much evidence of a wide range of pharmacological activity of the essential oil Laurus nobilis L. Scientists from different countries have confirmed antifun-gal and bactericidal activity [12], a beneficial effect on the digestive system [13]. In the literature there is information about the study of essential oil of Laurus nobilis L. leaves as a remedy against nematodes [14], there is information about cytotoxic [15] and antioxidant effects [4,16,17], extracts from raw Laurus nobilis L. showed in vitro antidiabetic activity [3]. Raw materials are also widely used in the manufacture of medicines, perfumes and cosmetics as a flavouring and as an insecticide [6,18,19].…”

Marine-Derived Penicillium purpurogenum Reduces Tumor Size and Ameliorates Inflammation in an Erlich Mice Model

“…New drugs are being developed from secondary metabolites to discover less toxic and more effective compounds compared to traditional cancer therapies [13]. Some natural products have also become important nutraceuticals with cancer chemopreventive properties [14,15].…”